SOCS3（suppressors of cytokine signaling 3）可以调节信号转导过程并影响造血干细胞的分化.利用生物信息学方法,发现SOCS3蛋白含有脯氨酸富集区,采用重组PCR技术缺失SOCS3蛋白中的129-161位的氨基酸（SOCS3Δ129-161）,并将其克隆到p...SOCS3（suppressors of cytokine signaling 3）可以调节信号转导过程并影响造血干细胞的分化.利用生物信息学方法,发现SOCS3蛋白含有脯氨酸富集区,采用重组PCR技术缺失SOCS3蛋白中的129-161位的氨基酸（SOCS3Δ129-161）,并将其克隆到pcDNA31/V5质粒载体中.转染细胞,然后通过流式细胞技术,分析预测的功能区对SOCS3蛋白功能的影响.结果表明,此脯氨酸富集区对SOCS3蛋白的三级结构和功能有重要的意义.建模结果显示,SOCS3Δ129-161蛋白结构发生显著变化.脯氨酸富集区的缺失也影响小鼠骨髓细胞向CD8^＋T细胞分化.研究结果显示,SOCS3蛋白中129-161位的脯氨酸富集区在其结构和功能上有重要的作用.展开更多
A simple recombinant PCR method was used to delete the dszB gene responsible for the slowest step of the Dsz pathway and allow the accumulation of hydroxyphenyl benzene sulfinate (HPBS) in the recombinant E. coli. Usi...A simple recombinant PCR method was used to delete the dszB gene responsible for the slowest step of the Dsz pathway and allow the accumulation of hydroxyphenyl benzene sulfinate (HPBS) in the recombinant E. coli. Using GC/MS, HPBS accumulation was confirmed. The recombinant E. coli could also desulfurize Cx-DBT to corresponding Cx-HPBS. The result gave a new insight to BDS process and explored a new method to obtain valuable surfactants from cheap raw materials.展开更多
文摘SOCS3（suppressors of cytokine signaling 3）可以调节信号转导过程并影响造血干细胞的分化.利用生物信息学方法,发现SOCS3蛋白含有脯氨酸富集区,采用重组PCR技术缺失SOCS3蛋白中的129-161位的氨基酸（SOCS3Δ129-161）,并将其克隆到pcDNA31/V5质粒载体中.转染细胞,然后通过流式细胞技术,分析预测的功能区对SOCS3蛋白功能的影响.结果表明,此脯氨酸富集区对SOCS3蛋白的三级结构和功能有重要的意义.建模结果显示,SOCS3Δ129-161蛋白结构发生显著变化.脯氨酸富集区的缺失也影响小鼠骨髓细胞向CD8^＋T细胞分化.研究结果显示,SOCS3蛋白中129-161位的脯氨酸富集区在其结构和功能上有重要的作用.
文摘A simple recombinant PCR method was used to delete the dszB gene responsible for the slowest step of the Dsz pathway and allow the accumulation of hydroxyphenyl benzene sulfinate (HPBS) in the recombinant E. coli. Using GC/MS, HPBS accumulation was confirmed. The recombinant E. coli could also desulfurize Cx-DBT to corresponding Cx-HPBS. The result gave a new insight to BDS process and explored a new method to obtain valuable surfactants from cheap raw materials.
