Background The induction of immune tolerance and suppression of allograft rejection has become the focus in the study of liver transplantation. The effect of immune therapy with anti-CD40L mAb alone or in combination ...Background The induction of immune tolerance and suppression of allograft rejection has become the focus in the study of liver transplantation. The effect of immune therapy with anti-CD40L mAb alone or in combination with cyclosporine A （CsA） on the recipient survival and Th1/Th2 cytokine profile was studied to elucidate its immunological mechanism and role in rat orthotopic liver transplantation.Methods The model of rat orthotopic liver transplantation was established by modified Kamada＇s technique. Recipients were divided into group A （control group）： SD→SD; group B （group of rejection）： SD→Wistar without any treatment; group C： SD→Wistar with CsA monotherapy from day 1 to day 5; and group D： SD→Wistar with CsA from day 1 to day 5 and anti-CD40L mAb on day 0 and day 2. The survival of the recipients in all groups was observed and ELISA technique was used to detect the level of cytokines in peripheral blood on post-transplant day 7.Results The survival period of recipients in groups A （〉60 days） and D （〉60 days） was significantly longer than that in group B （13.8±2.4 days）. The serum levels of interleukin 2 （IL-2） and interferon y in group B were significantly higher than those in other groups; the level of tumor necrosis factor a was higher but not statistically significant. In contrast, the serum levels of IL-4 and IL-10 in group D were elevated more significantly than those in group B （P〈0.05）.Conclusions Combined immune therapy can prolong the survival of al log rafts. Increased expression of Th2 cytokines, which is closely related to the induction of tolerance and suppression of rejection, is beneficial to the long-term survival of recipients and allografts.展开更多
目的系统评价和整合肺移植受者居家护理需求的质性研究,为制订以肺移植受者需求为导向的健康教育并开展延续性护理服务提供依据。方法检索PubMed、Embase、Web of Science、Cochrane Library、CINAHL、ProQuest、中国知网、万方数据库...目的系统评价和整合肺移植受者居家护理需求的质性研究,为制订以肺移植受者需求为导向的健康教育并开展延续性护理服务提供依据。方法检索PubMed、Embase、Web of Science、Cochrane Library、CINAHL、ProQuest、中国知网、万方数据库、维普数据库、中国生物医学文献数据库关于肺移植受者居家护理需求的质性研究,检索时限为建库至2020年8月。使用“澳大利亚乔安娜布里格斯研究所循证卫生保健中心质性研究质量评价标准(2016)”评价文献质量,采用Meta整合方法对结果进行整合。结果共纳入11篇研究,提炼出60个研究结果,归纳出11个新类别,最终综合为信息需求、生理需求、心理精神需求和社会需求4个整合结果。结论肺移植受者居家护理需求具有多样化、特殊性的特点。移植团队应高度重视肺移植受者的居家护理需求,根据需求情况为其提供居家教育、监测、肺康复指导和社会支持,进而提高肺移植受者的自我管理能力和生活质量。展开更多
文摘Background The induction of immune tolerance and suppression of allograft rejection has become the focus in the study of liver transplantation. The effect of immune therapy with anti-CD40L mAb alone or in combination with cyclosporine A （CsA） on the recipient survival and Th1/Th2 cytokine profile was studied to elucidate its immunological mechanism and role in rat orthotopic liver transplantation.Methods The model of rat orthotopic liver transplantation was established by modified Kamada＇s technique. Recipients were divided into group A （control group）： SD→SD; group B （group of rejection）： SD→Wistar without any treatment; group C： SD→Wistar with CsA monotherapy from day 1 to day 5; and group D： SD→Wistar with CsA from day 1 to day 5 and anti-CD40L mAb on day 0 and day 2. The survival of the recipients in all groups was observed and ELISA technique was used to detect the level of cytokines in peripheral blood on post-transplant day 7.Results The survival period of recipients in groups A （〉60 days） and D （〉60 days） was significantly longer than that in group B （13.8±2.4 days）. The serum levels of interleukin 2 （IL-2） and interferon y in group B were significantly higher than those in other groups; the level of tumor necrosis factor a was higher but not statistically significant. In contrast, the serum levels of IL-4 and IL-10 in group D were elevated more significantly than those in group B （P〈0.05）.Conclusions Combined immune therapy can prolong the survival of al log rafts. Increased expression of Th2 cytokines, which is closely related to the induction of tolerance and suppression of rejection, is beneficial to the long-term survival of recipients and allografts.
文摘目的系统评价和整合肺移植受者居家护理需求的质性研究,为制订以肺移植受者需求为导向的健康教育并开展延续性护理服务提供依据。方法检索PubMed、Embase、Web of Science、Cochrane Library、CINAHL、ProQuest、中国知网、万方数据库、维普数据库、中国生物医学文献数据库关于肺移植受者居家护理需求的质性研究,检索时限为建库至2020年8月。使用“澳大利亚乔安娜布里格斯研究所循证卫生保健中心质性研究质量评价标准(2016)”评价文献质量,采用Meta整合方法对结果进行整合。结果共纳入11篇研究,提炼出60个研究结果,归纳出11个新类别,最终综合为信息需求、生理需求、心理精神需求和社会需求4个整合结果。结论肺移植受者居家护理需求具有多样化、特殊性的特点。移植团队应高度重视肺移植受者的居家护理需求,根据需求情况为其提供居家教育、监测、肺康复指导和社会支持,进而提高肺移植受者的自我管理能力和生活质量。
