Naphthalene is a common environmental contaminant substance. The toxic effects of naphthalene on Caenorhabditis elegans were investigated at the molecular, biochemical and physiological levels. To assess the molecular...Naphthalene is a common environmental contaminant substance. The toxic effects of naphthalene on Caenorhabditis elegans were investigated at the molecular, biochemical and physiological levels. To assess the molecular-level effect, stress-related gene expression was investigated such as those of hsp-16.1, sod-3, ctl-2, cep-1, cyp35a2, ugt-44, gst-1 and dhs-28. Cell apoptosis was assessed at the biochemical level. Life span, locomotion behaviors and brood size were investigated at the physiological level. The results indicate that naphthalene exposure could not only induce the expression of stress-related genes such as hsp16.1, sod-3, ctl-2 and cep-1 but also reduce the life span of Caenorhabditis elegans. At the same time, naphthalene exposure could result in cell apoptosis and interfere in the locomotion behaviors of Caenorhabditis elegans. These data suggest that naphthalene has multiple toxicity on Caenorhabditis elegans.展开更多
In this study, biostimulation technology was used for bioremediation of nitrobenzene-contaminated groundwater by adding a mixture of lactose and phosphate, peptone, and beef extract. During the process of biostimulati...In this study, biostimulation technology was used for bioremediation of nitrobenzene-contaminated groundwater by adding a mixture of lactose and phosphate, peptone, and beef extract. During the process of biostimulation, the remediation effectiveness, microbial dehydrogenase activities and microbial densities were investigated; the varieties of microbial community structure and composition were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE） technique and the relative abundances of nitrobenzene-degrading gene（nbzA） were determined by fluorescence quantitative real-time PCR（RT-PCR）. Findings show that the removal rate of nitrobenzene in groundwater could reach about 60% by biostimulation with lactose and phosphate, 70% with peptone and 68% with beef extract. The microbial dehydrogenase activities and microbial densities were all improved obviously via biostimulation. The results of PCR-DGGE show that the microbial diversities were improved, and more than ten kinds of dominant microorganisms were detected after biostimulation. RT-PCR results show that the relative abundances of nbzA gene of microbes in groundwater were increased significantly, which indicated that biostimulation actually enhanced the growth of nitrobenzene-degrading bacteria. Therefore, biostimulation is a cost-effective and feasible bioremediation technique for nitrobenzene-contaminated groundwater.展开更多
基金Supported by the National Natural Science Foundation of China(No.20877017)the National High Technology Research and Development Program of China (No.2007AA03Z314)
文摘Naphthalene is a common environmental contaminant substance. The toxic effects of naphthalene on Caenorhabditis elegans were investigated at the molecular, biochemical and physiological levels. To assess the molecular-level effect, stress-related gene expression was investigated such as those of hsp-16.1, sod-3, ctl-2, cep-1, cyp35a2, ugt-44, gst-1 and dhs-28. Cell apoptosis was assessed at the biochemical level. Life span, locomotion behaviors and brood size were investigated at the physiological level. The results indicate that naphthalene exposure could not only induce the expression of stress-related genes such as hsp16.1, sod-3, ctl-2 and cep-1 but also reduce the life span of Caenorhabditis elegans. At the same time, naphthalene exposure could result in cell apoptosis and interfere in the locomotion behaviors of Caenorhabditis elegans. These data suggest that naphthalene has multiple toxicity on Caenorhabditis elegans.
基金Supported by the National Natural Science Foundation of China(No.41072170)the National High Technology Research and Development Program of China(No. 2007AA06A410)the Graduate Innovation Fund of Jilin University, China(No.20111037)
文摘In this study, biostimulation technology was used for bioremediation of nitrobenzene-contaminated groundwater by adding a mixture of lactose and phosphate, peptone, and beef extract. During the process of biostimulation, the remediation effectiveness, microbial dehydrogenase activities and microbial densities were investigated; the varieties of microbial community structure and composition were analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis（PCR-DGGE） technique and the relative abundances of nitrobenzene-degrading gene（nbzA） were determined by fluorescence quantitative real-time PCR（RT-PCR）. Findings show that the removal rate of nitrobenzene in groundwater could reach about 60% by biostimulation with lactose and phosphate, 70% with peptone and 68% with beef extract. The microbial dehydrogenase activities and microbial densities were all improved obviously via biostimulation. The results of PCR-DGGE show that the microbial diversities were improved, and more than ten kinds of dominant microorganisms were detected after biostimulation. RT-PCR results show that the relative abundances of nbzA gene of microbes in groundwater were increased significantly, which indicated that biostimulation actually enhanced the growth of nitrobenzene-degrading bacteria. Therefore, biostimulation is a cost-effective and feasible bioremediation technique for nitrobenzene-contaminated groundwater.
