A tooth is a complex biological organ and consists of multiple tissues including the enamel, dentin, cementum and pulp. Tooth loss is the most common organ failure. Can a tooth be regenerated？ Can adult stem cells be...A tooth is a complex biological organ and consists of multiple tissues including the enamel, dentin, cementum and pulp. Tooth loss is the most common organ failure. Can a tooth be regenerated？ Can adult stem cells be orchestrated to regenerate tooth structures such as the enamel, dentin, cementum and dental pulp, or even an entire tooth？ If not, what are the therapeutically viable sources of stem cells for tooth regeneration？ Do stem cells necessarily need to be taken out of the body, and manipulated ex vivo before they are transplanted for tooth regeneration？ How can regenerated teeth be economically competitive with dental implants？ Would it be possible to make regenerated teeth affordable by a large segment of the population worldwide？ This review article explores existing and visionary approaches that address some of the above-mentioned questions. Tooth regeneration represents a revolution in stomatology as a shift in the paradigm from repair to regeneration： repair is by metal or artificial materials whereas regeneration is by biological restoration. Tooth regeneration is an extension of the concepts in the broad field of regenerative medicine to restore a tissue defect to its original form and function by biological substitutes.展开更多
目的分析年轻恒牙急性牙髓炎再生性牙髓治疗中选择不同支架材料的疗效。方法将我院2017年1月至2018年2月收治的72例(72牙)龋齿、80例(88牙)外伤致年轻恒牙急性牙髓炎患儿,采用随机数字表法分为4个亚组,分别采用三氧化矿物聚合物(MTA)、...目的分析年轻恒牙急性牙髓炎再生性牙髓治疗中选择不同支架材料的疗效。方法将我院2017年1月至2018年2月收治的72例(72牙)龋齿、80例(88牙)外伤致年轻恒牙急性牙髓炎患儿,采用随机数字表法分为4个亚组,分别采用三氧化矿物聚合物(MTA)、生物陶瓷材料(iRoot BP Plus)、MTA+富血小板纤维蛋白(PRF)、MTA+脱细胞真皮基质(ADM)支架材料进行牙髓手术。比较各组手术前后患牙检查指标及术后各亚组间疗效。结果龋齿组与外伤组术后总有效率分别为70.83%(51/72)、65.91%(58/88),组间差异无统计学意义(P>0.05);龋齿组与外伤组的各亚组总有效率比较,均显示PRF+MTA亚组较MTA亚组、iRoot BP Plus亚组总有效率明显提高(P<0.05);龋齿组、外伤组的各亚组根尖根管改善情况比较,均显示PRF+MTA亚组根管长度、根管壁厚度增加明显优于MTA亚组与iRoot BP Plus亚组(P<0.05);患牙恢复情况比较,龋齿组的PRF+MTA亚组根尖孔封闭、牙髓活力正常、牙体色泽正常、萌出高度增加率均明显高于MTA亚组与iRoot BP Plus亚组(P<0.05);外伤组的PRF+MTA亚组根尖孔封闭、牙髓活力正常、牙体色泽正常率均明显高于MTA亚组(P<0.05)。结论年轻恒牙急性牙髓炎采用PRF+MTA支架进行再生性牙髓治疗,能更好地恢复牙髓活力,促进根尖孔封闭。展开更多
背景:现有研究已经证实外泌体可有效促进牙髓再生,而经预处理来源的外泌体其生物学功能和特性会发生显著改变,对细胞的增殖、迁移和成牙分化产生不同的影响。目的:探讨外泌体及其预处理方式在牙髓再生领域的应用现状,归纳和总结影响外...背景:现有研究已经证实外泌体可有效促进牙髓再生,而经预处理来源的外泌体其生物学功能和特性会发生显著改变,对细胞的增殖、迁移和成牙分化产生不同的影响。目的:探讨外泌体及其预处理方式在牙髓再生领域的应用现状,归纳和总结影响外泌体发挥作用的预处理方式,并阐述外泌体及其预处理方式对牙髓再生的作用。方法:检索万方、中国知网、PubMed和Web of Science数据库中2006-2022年发表的相关文献,以“外泌体,牙髓再生,预处理方式”等为中文检索词,以“Exosomes,Pulp regeneration,Preconditioning method”等为英文检索词进行检索,共纳入78篇文献进行综述分析。结果与结论:①外泌体具有良好的生物相容性、低免疫原性和无细胞毒性等优势,可以通过促进干细胞成牙、成神经和成血管化进而诱导牙髓组织的新生。②经预处理衍生的外泌体可以增强对组织的修复和再生能力,并对再生牙髓的质量有显著影响。③目前应用在牙髓再生领域中的预处理方式包括炎症刺激、低氧诱导、条件培养基和三维培养,其分泌的外泌体均能有效改善再生牙髓的质量,但是不同的预处理方式对牙髓再生的具体效果和机制在未来尚需探索。展开更多
基金supported by RC2DE020767 from the National Institute of Dental and Craniofacial Research (NIDCR), the National Institutes of Health (NIH)
文摘A tooth is a complex biological organ and consists of multiple tissues including the enamel, dentin, cementum and pulp. Tooth loss is the most common organ failure. Can a tooth be regenerated？ Can adult stem cells be orchestrated to regenerate tooth structures such as the enamel, dentin, cementum and dental pulp, or even an entire tooth？ If not, what are the therapeutically viable sources of stem cells for tooth regeneration？ Do stem cells necessarily need to be taken out of the body, and manipulated ex vivo before they are transplanted for tooth regeneration？ How can regenerated teeth be economically competitive with dental implants？ Would it be possible to make regenerated teeth affordable by a large segment of the population worldwide？ This review article explores existing and visionary approaches that address some of the above-mentioned questions. Tooth regeneration represents a revolution in stomatology as a shift in the paradigm from repair to regeneration： repair is by metal or artificial materials whereas regeneration is by biological restoration. Tooth regeneration is an extension of the concepts in the broad field of regenerative medicine to restore a tissue defect to its original form and function by biological substitutes.
文摘目的分析年轻恒牙急性牙髓炎再生性牙髓治疗中选择不同支架材料的疗效。方法将我院2017年1月至2018年2月收治的72例(72牙)龋齿、80例(88牙)外伤致年轻恒牙急性牙髓炎患儿,采用随机数字表法分为4个亚组,分别采用三氧化矿物聚合物(MTA)、生物陶瓷材料(iRoot BP Plus)、MTA+富血小板纤维蛋白(PRF)、MTA+脱细胞真皮基质(ADM)支架材料进行牙髓手术。比较各组手术前后患牙检查指标及术后各亚组间疗效。结果龋齿组与外伤组术后总有效率分别为70.83%(51/72)、65.91%(58/88),组间差异无统计学意义(P>0.05);龋齿组与外伤组的各亚组总有效率比较,均显示PRF+MTA亚组较MTA亚组、iRoot BP Plus亚组总有效率明显提高(P<0.05);龋齿组、外伤组的各亚组根尖根管改善情况比较,均显示PRF+MTA亚组根管长度、根管壁厚度增加明显优于MTA亚组与iRoot BP Plus亚组(P<0.05);患牙恢复情况比较,龋齿组的PRF+MTA亚组根尖孔封闭、牙髓活力正常、牙体色泽正常、萌出高度增加率均明显高于MTA亚组与iRoot BP Plus亚组(P<0.05);外伤组的PRF+MTA亚组根尖孔封闭、牙髓活力正常、牙体色泽正常率均明显高于MTA亚组(P<0.05)。结论年轻恒牙急性牙髓炎采用PRF+MTA支架进行再生性牙髓治疗,能更好地恢复牙髓活力,促进根尖孔封闭。
文摘背景:现有研究已经证实外泌体可有效促进牙髓再生,而经预处理来源的外泌体其生物学功能和特性会发生显著改变,对细胞的增殖、迁移和成牙分化产生不同的影响。目的:探讨外泌体及其预处理方式在牙髓再生领域的应用现状,归纳和总结影响外泌体发挥作用的预处理方式,并阐述外泌体及其预处理方式对牙髓再生的作用。方法:检索万方、中国知网、PubMed和Web of Science数据库中2006-2022年发表的相关文献,以“外泌体,牙髓再生,预处理方式”等为中文检索词,以“Exosomes,Pulp regeneration,Preconditioning method”等为英文检索词进行检索,共纳入78篇文献进行综述分析。结果与结论:①外泌体具有良好的生物相容性、低免疫原性和无细胞毒性等优势,可以通过促进干细胞成牙、成神经和成血管化进而诱导牙髓组织的新生。②经预处理衍生的外泌体可以增强对组织的修复和再生能力,并对再生牙髓的质量有显著影响。③目前应用在牙髓再生领域中的预处理方式包括炎症刺激、低氧诱导、条件培养基和三维培养,其分泌的外泌体均能有效改善再生牙髓的质量,但是不同的预处理方式对牙髓再生的具体效果和机制在未来尚需探索。
