The hollow structure has long attracted great attention because of its excellent properties.However,this special structure is usually synthesized through some complex approaches.Herein,we discovered that denatured bov...The hollow structure has long attracted great attention because of its excellent properties.However,this special structure is usually synthesized through some complex approaches.Herein,we discovered that denatured bovine serum albumin(BSA)can trigger unusual biomineralization for the simple,green and shape-controllable synthesis of germanium oxide(GeOx)hollow microsphere(HMS).At high temperature(60℃),BSA was denatured,and a compact BSA layer was formed around the H2 bubbles.The denatured BSA layer was stable and suitable for anchoring and growing GeOx.By simply changing the BSA concentration and temperature,various morphologies of GeOx could be obtained.Due to the denatured protein skeletons and microenvironment-regulated collapse,GeOx HMS showed great potential for intelligently responsive pesticide delivery in the insect gut,showing superiority over traditional delivery systems,which early release pesticides in the mouth and stomach.Inspired by its large specific surface area,excellent biocompatibility,modifiable functional groups,and high electrocatalytic activity,GeOx HMS was also applied to versatile sensors for H_(2)O_(2) assays at physiological pH and rapid coronavirus COVID-19 detection.This work not only provides some evidence for understanding proteins in depth but also paves a new avenue for the biomineralization-inspired synthesis of hollow structures with versatile functions.展开更多
Sensitive and specific imaging of tumor-associated biomarkers offers an effective way for cancer diagnosis.However,most of the reported imaging probes are limited to the targeting of a single biomarker,which may impai...Sensitive and specific imaging of tumor-associated biomarkers offers an effective way for cancer diagnosis.However,most of the reported imaging probes are limited to the targeting of a single biomarker,which may impair imaging precision.Here,we report an aptamer-based,AND-gate DNA nanodevice that relies on the sequential processing of two tumor-associated proteins to produce a specific fluorescent signal for precise tumor imaging.The DNA nanodevice integrates an AS1411 aptamer to bind cell surface nucleolin for tumor targeting and a DNA sensor to respond to intracellular apurinic/apyrimidinic endonuclease 1 for activatable imaging.We evaluated the performance of the system in both living cells and a tumor-bearing mouse model,and demonstrated its capability in tumor cell-specific imaging.We anticipate that this strategy will accelerate the design of multivariate protein-stimulated probes for tumor diagnosis.展开更多
Cancer activated protein-inorganic nanoparticles can be cancer targeting turn-on imaging and therapy agents. Although various techniques were successfully employed for development of hybrid proteininorganic nanopartic...Cancer activated protein-inorganic nanoparticles can be cancer targeting turn-on imaging and therapy agents. Although various techniques were successfully employed for development of hybrid proteininorganic nanoparticles, cancer cell activated protein-inorganic nanoparticles have been challengeable.Herein, a cancer cell responsive nanoparticle（PDAMn-CuS@BSA-FA, NPs） was constructed and characterized. It is found that the quenching interaction of dyes（PDA） and central metal in NPs can be adjusted by CH_3O-PEG-phosphatide or the cancer cells, hence, NPs showed turn-on fluorescence emission with the titration of CH_3O-PEG-phosphatide. In particular, breast cancer cells lysis can switch on the green emission greatly, while normal cells show less effect. Breast cancer cells turn-on fluorescence imaging and mitochondria targeting imaging demonstrate that the NPs can sense breast cancer cells and enter mitochondria. Therefore, NPs can be both breast cancer targeting nanosensor.展开更多
Electronic sensors based on biomaterials can lead to novel green technologies that are low cost,renewable,and eco-friendly.Here we demonstrate bioelectronic ammonia sensors made from protein nanowires harvested from t...Electronic sensors based on biomaterials can lead to novel green technologies that are low cost,renewable,and eco-friendly.Here we demonstrate bioelectronic ammonia sensors made from protein nanowires harvested from the microorganism Geobacter sulfurreducens.The nanowire sensor responds to a broad range of ammonia concentrations(10 to 10^6 ppb),which covers the range relevant for industrial,environmental,and biomedical applications.The sensor also demonstrates high selectivity to ammonia compared to moisture and other common gases found in human breath.These results provide a proof-of-concept demonstration for developing protein nanowire based gas sensors for applications in industry,agriculture,environmental monitoring,and healthcare.展开更多
In this paper a label-free fluorescent sensor for probing the interaction between heparin and protein was reported.Heparin,the bioactive polyanions,formed supramolecular assemblies with cationic surfactant cetyltrimet...In this paper a label-free fluorescent sensor for probing the interaction between heparin and protein was reported.Heparin,the bioactive polyanions,formed supramolecular assemblies with cationic surfactant cetyltrimethyl ammonium bromide (CTAB).The environment-dependent dye pyrene,encapsulated in hydrophobic interiors of the supramolecular assemblies worked as the fluorescence probe.Once the heparin-binding protein was added,competing interactions of protein with heparin would weaken the interaction between CTAB and heparin.As a result,the noncovalently sequestered pyrene would be released upon disassembly and the fluorescence of the released pyrene was subsequently decreased.The binding events were exemplified by protamine and Tat peptide,these processes were also verified by DLS and TEM.Such a strategy is appealing as organic synthesis was traded off against supramolecular assembly.This label-free fluorescent system is simple,selective,convenient,and can serve as a good complement to other existing methods,also this method has the potential for preprimary drug screening.展开更多
This paper presents a high specific, sensitive electrochemical biosensor for recognition of protein such as thrombin based on aptamers and nano particles. Two different aptamers were chosen to construct a sandwich man...This paper presents a high specific, sensitive electrochemical biosensor for recognition of protein such as thrombin based on aptamers and nano particles. Two different aptamers were chosen to construct a sandwich manner for detecting thrombin. Aptamer I was immobilized on nano magnetic particle for capturing thrombin, and aptamer II labled with nano gold was used for detection. The electrical current generated from gold after the formation of the complex of magnetic particle, thrombin and nano gold, and then an electrochemical cell designed by ourselves was used for separating, gathering, and electrochemical detecting. Through magnetic separation, high specific and sensitive detection of the target protein, thrombin, was achieved. Linear response was observed over the range 5.6×10-12―1.12×10-9 mol/L, with a detection limit of 1.42×10-12 mol/L. The presence of other protein as BSA did not affect the detection, which indicates that high selective recognition of thrombin can be achieved in complex biological samples such as human plasma.展开更多
The multi-step phosphorelay （MSP） system defines a key signal transduction pathway in plants and many eukaryotes. In this system, external stimuli first lead to the activation of a histidine kinase, followed by tran...The multi-step phosphorelay （MSP） system defines a key signal transduction pathway in plants and many eukaryotes. In this system, external stimuli first lead to the activation of a histidine kinase, followed by transfer of a phosphoryl group from the receiver domain of the kinase （HKRD） to downstream, cytosolic phosphotransfer proteins （HPs）. In order to establish the determinants of specificity for this signaling relay system, we have solved the first crystal structure of a plant HKRD, AHK5RD, in complex with one of its cognate HPs, AHP1. AHP1 binds AHK5RD via a prominent hydrogen bond docking ridge and a hydrophobic patch. These features are conserved among all AHP proteins, but differ significantly from other structurally characterized prokaryotic and eukaryotic HPs. Surface plasmon resonance experiments show that AHK5RD binds to AHP1-3 with similar, micromolar affinity, consistent with the transient nature of this signaling complex. Our correlation of structural and functional data provide the first insight, at the atomic level as well as with quantitative affinity data, into the molecular recognition events governing the MSP in plants.展开更多
基金National Natural Science Foundation of China,Grant/Award Number:21705087Natural Science Foundation of Shandong Province,Grant/Award Number:ZR2021MB018+1 种基金Research Foundation for Distinguished Scholars of Qingdao Agricultural University,Grant/Award Number:663-1117015Introducing and Breeding Plan of Shandong Province Higher Educational Youth Innovation Talents(Shandong Provincial Department of Education)。
文摘The hollow structure has long attracted great attention because of its excellent properties.However,this special structure is usually synthesized through some complex approaches.Herein,we discovered that denatured bovine serum albumin(BSA)can trigger unusual biomineralization for the simple,green and shape-controllable synthesis of germanium oxide(GeOx)hollow microsphere(HMS).At high temperature(60℃),BSA was denatured,and a compact BSA layer was formed around the H2 bubbles.The denatured BSA layer was stable and suitable for anchoring and growing GeOx.By simply changing the BSA concentration and temperature,various morphologies of GeOx could be obtained.Due to the denatured protein skeletons and microenvironment-regulated collapse,GeOx HMS showed great potential for intelligently responsive pesticide delivery in the insect gut,showing superiority over traditional delivery systems,which early release pesticides in the mouth and stomach.Inspired by its large specific surface area,excellent biocompatibility,modifiable functional groups,and high electrocatalytic activity,GeOx HMS was also applied to versatile sensors for H_(2)O_(2) assays at physiological pH and rapid coronavirus COVID-19 detection.This work not only provides some evidence for understanding proteins in depth but also paves a new avenue for the biomineralization-inspired synthesis of hollow structures with versatile functions.
基金financially supported by the Beijing Natural Science Foundation(JQ20005).
文摘Sensitive and specific imaging of tumor-associated biomarkers offers an effective way for cancer diagnosis.However,most of the reported imaging probes are limited to the targeting of a single biomarker,which may impair imaging precision.Here,we report an aptamer-based,AND-gate DNA nanodevice that relies on the sequential processing of two tumor-associated proteins to produce a specific fluorescent signal for precise tumor imaging.The DNA nanodevice integrates an AS1411 aptamer to bind cell surface nucleolin for tumor targeting and a DNA sensor to respond to intracellular apurinic/apyrimidinic endonuclease 1 for activatable imaging.We evaluated the performance of the system in both living cells and a tumor-bearing mouse model,and demonstrated its capability in tumor cell-specific imaging.We anticipate that this strategy will accelerate the design of multivariate protein-stimulated probes for tumor diagnosis.
基金financially supported by the National Natural Science Foundation of China (No. 21571085)
文摘Cancer activated protein-inorganic nanoparticles can be cancer targeting turn-on imaging and therapy agents. Although various techniques were successfully employed for development of hybrid proteininorganic nanoparticles, cancer cell activated protein-inorganic nanoparticles have been challengeable.Herein, a cancer cell responsive nanoparticle（PDAMn-CuS@BSA-FA, NPs） was constructed and characterized. It is found that the quenching interaction of dyes（PDA） and central metal in NPs can be adjusted by CH_3O-PEG-phosphatide or the cancer cells, hence, NPs showed turn-on fluorescence emission with the titration of CH_3O-PEG-phosphatide. In particular, breast cancer cells lysis can switch on the green emission greatly, while normal cells show less effect. Breast cancer cells turn-on fluorescence imaging and mitochondria targeting imaging demonstrate that the NPs can sense breast cancer cells and enter mitochondria. Therefore, NPs can be both breast cancer targeting nanosensor.
基金J.Y.and D.R.L.acknowledge support from a seed fund through the Office of Technology Commercialization and Ventures at the University of Massachusetts,Amherst.J.Y.acknowledges the support from a National Science Foundation(NSF)Award ECCS-1917630.J.M.J.acknowledges support from a NSF grants CAREER CMMI184230&A.F.S.acknowledges the support from a NSF Graduate Research Fellowship(No.S12100000000937).Part of the device fabrication work was conducted in the clean room of the Center for Hierarchical Manufacturing(CHM),an NSF Nanoscale Science and Engineering Center(NSEC)located at the University of Massachusetts,Amherst.
文摘Electronic sensors based on biomaterials can lead to novel green technologies that are low cost,renewable,and eco-friendly.Here we demonstrate bioelectronic ammonia sensors made from protein nanowires harvested from the microorganism Geobacter sulfurreducens.The nanowire sensor responds to a broad range of ammonia concentrations(10 to 10^6 ppb),which covers the range relevant for industrial,environmental,and biomedical applications.The sensor also demonstrates high selectivity to ammonia compared to moisture and other common gases found in human breath.These results provide a proof-of-concept demonstration for developing protein nanowire based gas sensors for applications in industry,agriculture,environmental monitoring,and healthcare.
基金Financial support from the Natural Science Foundation of China (Nos.50830106,21174126),the China National Funds for Distinguished Young Scientists (No.51025312) and the Open Project of State Key Laboratory of Supramolecular Structure and Materials (No.sklssm201316) is gratefully acknowledged.
文摘In this paper a label-free fluorescent sensor for probing the interaction between heparin and protein was reported.Heparin,the bioactive polyanions,formed supramolecular assemblies with cationic surfactant cetyltrimethyl ammonium bromide (CTAB).The environment-dependent dye pyrene,encapsulated in hydrophobic interiors of the supramolecular assemblies worked as the fluorescence probe.Once the heparin-binding protein was added,competing interactions of protein with heparin would weaken the interaction between CTAB and heparin.As a result,the noncovalently sequestered pyrene would be released upon disassembly and the fluorescence of the released pyrene was subsequently decreased.The binding events were exemplified by protamine and Tat peptide,these processes were also verified by DLS and TEM.Such a strategy is appealing as organic synthesis was traded off against supramolecular assembly.This label-free fluorescent system is simple,selective,convenient,and can serve as a good complement to other existing methods,also this method has the potential for preprimary drug screening.
文摘This paper presents a high specific, sensitive electrochemical biosensor for recognition of protein such as thrombin based on aptamers and nano particles. Two different aptamers were chosen to construct a sandwich manner for detecting thrombin. Aptamer I was immobilized on nano magnetic particle for capturing thrombin, and aptamer II labled with nano gold was used for detection. The electrical current generated from gold after the formation of the complex of magnetic particle, thrombin and nano gold, and then an electrochemical cell designed by ourselves was used for separating, gathering, and electrochemical detecting. Through magnetic separation, high specific and sensitive detection of the target protein, thrombin, was achieved. Linear response was observed over the range 5.6×10-12―1.12×10-9 mol/L, with a detection limit of 1.42×10-12 mol/L. The presence of other protein as BSA did not affect the detection, which indicates that high selective recognition of thrombin can be achieved in complex biological samples such as human plasma.
文摘The multi-step phosphorelay （MSP） system defines a key signal transduction pathway in plants and many eukaryotes. In this system, external stimuli first lead to the activation of a histidine kinase, followed by transfer of a phosphoryl group from the receiver domain of the kinase （HKRD） to downstream, cytosolic phosphotransfer proteins （HPs）. In order to establish the determinants of specificity for this signaling relay system, we have solved the first crystal structure of a plant HKRD, AHK5RD, in complex with one of its cognate HPs, AHP1. AHP1 binds AHK5RD via a prominent hydrogen bond docking ridge and a hydrophobic patch. These features are conserved among all AHP proteins, but differ significantly from other structurally characterized prokaryotic and eukaryotic HPs. Surface plasmon resonance experiments show that AHK5RD binds to AHP1-3 with similar, micromolar affinity, consistent with the transient nature of this signaling complex. Our correlation of structural and functional data provide the first insight, at the atomic level as well as with quantitative affinity data, into the molecular recognition events governing the MSP in plants.
