Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major...Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.展开更多
We investigate oscillation of certain second order neutral dynamic equations of Emden-Fowler type with positive and negative coefficients. We use some different techniques and apply Riccati transformation to establish...We investigate oscillation of certain second order neutral dynamic equations of Emden-Fowler type with positive and negative coefficients. We use some different techniques and apply Riccati transformation to establish new oscillatory criteria which include two necessary and sufficient conditions. Moreover, we point out that how the power γ plays its role. Some interesting examples are given to illustrate the versatility of our results.展开更多
A splitting positive definite mixed finite element method is proposed for second-order viscoelasticity wave equation. The proposed procedure can be split into three independent symmetric positive definite integro-diff...A splitting positive definite mixed finite element method is proposed for second-order viscoelasticity wave equation. The proposed procedure can be split into three independent symmetric positive definite integro-differential sub-system and does not need to solve a coupled system of equations. Error estimates are derived for both semidiscrete and fully discrete schemes. The existence and uniqueness for semidiscrete scheme are proved. Finally, a numerical example is provided to illustrate the efficiency of the method.展开更多
Space and time traveling is one of the humanity’s most fascinating and challenging topics. The speed limitation makes space traveling highly difficult. Therefore, discovering the warp drive mechanism intrigued humani...Space and time traveling is one of the humanity’s most fascinating and challenging topics. The speed limitation makes space traveling highly difficult. Therefore, discovering the warp drive mechanism intrigued humanity to travel in space and time. Miguel Alcubierre proposed a model for warp drive. However, the energy density driven from the Alcubierre warp drive model turns to be negative everywhere. Erik Lentz proposed a shifting vector field in which we shall show that shifting vector field with appropriate spaceship geometry may provide positive energy density for warp drive. Further, we suggest looking at the spaceship geometry as a mother wavelet function with shifting, scaling, and rotation operations that may provide additional positive energy density. This sort of design requires a flexible fuselage that can be stretched and rotated.展开更多
We will discuss the non-normal Hasemann boundary value problem: we may find these results are coincided with those of normal Hasemann boundary value problem and non-normal Riemann boundary value problem.
At present,there is a problem of false positives caused by the too vast mimic scope in mimic transformation technology.Previous studies have focused on the“compensation”method to deal with this problem,which is expe...At present,there is a problem of false positives caused by the too vast mimic scope in mimic transformation technology.Previous studies have focused on the“compensation”method to deal with this problem,which is expensive and cannot fundamentally solve it.This paper provides new insights into coping with the situation.Firstly,this study summarizes the false-positive problem in the mimic transformation,analyzes its possible harm and the root causes.Secondly,three properties about the mimic scope are proposed.Based on the three properties and security quantification technology,the best mimic component set theory is put forward to solve the false-positive problem.There are two algorithms,the supplemental method and the subtraction method.The best mimic component set obtained by these two algorithms can fundamentally solve the mimic system’s false-positive problem but reduce the cost of mimic transformation.Thus make up for the lack of previous researches.展开更多
基金国家电网公司科技项目(<提高特高压互感器可靠性技术研究>)(合同编号EPRIGYKJ[2012]4939号)Science and Technology Project of the State Grid Corporation of China("Research to Improve the Reliability of UHV Instrument Transformer")(No.EPRIGYKJ[2012]4939)
基金financially supported by grants from the Biogreen 21 Program, RDA, Korea (PJ00810304)the Agricultural Science and Technology Innovation Program (ASTIP) of Chinese Academy of Agricultural Sciences (2014–2015)the Beijing Municipal Education Commission, China (KM200910011001)
文摘Genetic transformation is an important technique for functional genomics study and genetic improvement of plants. Until now, Agrobacterium-mediated transformation methods using cotyledon as explants has been the major approach for cucumber, and its frequency has been up to 23%. For example, significantly enhancement of the transformation efficiency of this plant species was achieved from the cotyledon explants of the cultivar Poinsett 76 infected by Agrobacterium strains EHA105 with efficient positive selection system in lots of experiments. This review is to summarize some key factors influencing cucumber regeneration and genetic transformation, including target genes, selection systems and the ways of transgene introduction, and then to put forward some strategies for the increasing of cucumber transformation efficiency. In the future, it is high possible for cucumber to be potential bioreactor to produce vaccine and biomaterials for human beings.
基金supported by National Natural Science Foundation of China (Grant No. 11271379)Guangzhou Postdoctoral Science Research Foundation Project (Grant No. gdbsh2014003)
文摘We investigate oscillation of certain second order neutral dynamic equations of Emden-Fowler type with positive and negative coefficients. We use some different techniques and apply Riccati transformation to establish new oscillatory criteria which include two necessary and sufficient conditions. Moreover, we point out that how the power γ plays its role. Some interesting examples are given to illustrate the versatility of our results.
基金This work was supported in part by the National Natural Science Foundation of China （Grant No, 11061021）, the Program of Higher-level Talents of Inner Mongolia University （No. Z200901004）, and the Scientific Research Projection of Higher Schools of Inner Mongolia （Nos. N J10006, N J10016, NJZZ12011）.
文摘A splitting positive definite mixed finite element method is proposed for second-order viscoelasticity wave equation. The proposed procedure can be split into three independent symmetric positive definite integro-differential sub-system and does not need to solve a coupled system of equations. Error estimates are derived for both semidiscrete and fully discrete schemes. The existence and uniqueness for semidiscrete scheme are proved. Finally, a numerical example is provided to illustrate the efficiency of the method.
文摘Space and time traveling is one of the humanity’s most fascinating and challenging topics. The speed limitation makes space traveling highly difficult. Therefore, discovering the warp drive mechanism intrigued humanity to travel in space and time. Miguel Alcubierre proposed a model for warp drive. However, the energy density driven from the Alcubierre warp drive model turns to be negative everywhere. Erik Lentz proposed a shifting vector field in which we shall show that shifting vector field with appropriate spaceship geometry may provide positive energy density for warp drive. Further, we suggest looking at the spaceship geometry as a mother wavelet function with shifting, scaling, and rotation operations that may provide additional positive energy density. This sort of design requires a flexible fuselage that can be stretched and rotated.
基金the National Natural Science Foundation of ChinaRF DP of Higher Education and SF of Wuhan Uni-versity(2 0 1990 3 3 6)
文摘We will discuss the non-normal Hasemann boundary value problem: we may find these results are coincided with those of normal Hasemann boundary value problem and non-normal Riemann boundary value problem.
基金This work was supported by National Key Research and Development Program of China(Grant No.2018YF0804001).
文摘At present,there is a problem of false positives caused by the too vast mimic scope in mimic transformation technology.Previous studies have focused on the“compensation”method to deal with this problem,which is expensive and cannot fundamentally solve it.This paper provides new insights into coping with the situation.Firstly,this study summarizes the false-positive problem in the mimic transformation,analyzes its possible harm and the root causes.Secondly,three properties about the mimic scope are proposed.Based on the three properties and security quantification technology,the best mimic component set theory is put forward to solve the false-positive problem.There are two algorithms,the supplemental method and the subtraction method.The best mimic component set obtained by these two algorithms can fundamentally solve the mimic system’s false-positive problem but reduce the cost of mimic transformation.Thus make up for the lack of previous researches.
