Variations in the trnK region of chloroplast DNA were investigated in the present study using polymerase chain reactionrestriction fragment length polymorphism to detect the genetic structure and to infer the possible...Variations in the trnK region of chloroplast DNA were investigated in the present study using polymerase chain reactionrestriction fragment length polymorphism to detect the genetic structure and to infer the possible glacial refugia of Ginkgo biloba L. in China. In total, 220 individuals from 12 populations in China and three populations outside China were analyzed, representing the largest number of populations studied by molecular markers to date. Nineteen haplotypes were produced and haplotype A was found in all populations. Populations in south-western China, including WC, JF, PX, and SP, contained 14 of the 19 haplotypes and their genetic diversity ranged from 0.771 4 to 0.867 6. The TM population from China also showed a high genetic diversity （H = 0.848 5）. Most of the genetic variation existed within populations and the differentiation among populations was low （GsT = 0.2）. According to haplotype distribution and the historical record, we suggest that populations of G. biloba have been subjected to extensive human impact, which has compounded our attempt to infer glacial refugia for Ginkgo. Nevertheless, the present results suggest that the center of genetic diversity of Ginkgo is mainly in south-western China and in situ conservation is needed to protect and preserve the genetic resources.展开更多
目的探讨山东地区亲代亚甲基四氢叶酸还原酶（methylenetetrahydrofolate reductase，MTHFR）基因677C／T多态性与子代发生非综合征性唇腭裂（nonsyndromic cleft lip with or without cleft palate，NSCL／P）的关联。方法应用聚合酶...目的探讨山东地区亲代亚甲基四氢叶酸还原酶（methylenetetrahydrofolate reductase，MTHFR）基因677C／T多态性与子代发生非综合征性唇腭裂（nonsyndromic cleft lip with or without cleft palate，NSCL／P）的关联。方法应用聚合酶链反应一限制性片段长度多态性分析技术（polymerasechainreaction—restrictionfragmentlengthpolymorphism，PCR-RFLP）对2006年8月至2008年8月在齐鲁医院治疗的89对NSCL／P患者亲代和64对健康查体儿童亲代的MTHFR基因677C／T多态性进行检测。结果患者母亲与正常儿童母亲的T等位基因频率分别为65．73％和46．09％，C等位基因频率分别为34．27％和53．91％，其构成比差异有统计学意义（x2=13．663，P〈O．01）；携带T等位基因的母亲子代患NSCL／P的风险为未携带T等位基因的母亲子代的2．243倍（95％CI：1．408~3．572）。患者的父亲与正常儿童父亲的T等位基因频率分别为62．92％和55．47％；C等位基因频率分别为37．08％和44．53％，其构成比差异无统计学意义（Y。=2．222，P〉0．05）；病例组和对照组后代可能为纯合突变胎儿的机率分别为43％和29％（P〉0．05）。结论山东地区母亲的MTHFR基因677C／T突变对后代NSCL／P的发生有重要的影响；父亲的MTHFR基因677C／T突变则可能不是子代患NSCL／P的风险因素。展开更多
基金the National Basic Research Program of China (973 Program2007CB411605)the National Natural Science Foundation of China(30670139, 30300020).
文摘Variations in the trnK region of chloroplast DNA were investigated in the present study using polymerase chain reactionrestriction fragment length polymorphism to detect the genetic structure and to infer the possible glacial refugia of Ginkgo biloba L. in China. In total, 220 individuals from 12 populations in China and three populations outside China were analyzed, representing the largest number of populations studied by molecular markers to date. Nineteen haplotypes were produced and haplotype A was found in all populations. Populations in south-western China, including WC, JF, PX, and SP, contained 14 of the 19 haplotypes and their genetic diversity ranged from 0.771 4 to 0.867 6. The TM population from China also showed a high genetic diversity （H = 0.848 5）. Most of the genetic variation existed within populations and the differentiation among populations was low （GsT = 0.2）. According to haplotype distribution and the historical record, we suggest that populations of G. biloba have been subjected to extensive human impact, which has compounded our attempt to infer glacial refugia for Ginkgo. Nevertheless, the present results suggest that the center of genetic diversity of Ginkgo is mainly in south-western China and in situ conservation is needed to protect and preserve the genetic resources.
文摘目的探讨山东地区亲代亚甲基四氢叶酸还原酶（methylenetetrahydrofolate reductase，MTHFR）基因677C／T多态性与子代发生非综合征性唇腭裂（nonsyndromic cleft lip with or without cleft palate，NSCL／P）的关联。方法应用聚合酶链反应一限制性片段长度多态性分析技术（polymerasechainreaction—restrictionfragmentlengthpolymorphism，PCR-RFLP）对2006年8月至2008年8月在齐鲁医院治疗的89对NSCL／P患者亲代和64对健康查体儿童亲代的MTHFR基因677C／T多态性进行检测。结果患者母亲与正常儿童母亲的T等位基因频率分别为65．73％和46．09％，C等位基因频率分别为34．27％和53．91％，其构成比差异有统计学意义（x2=13．663，P〈O．01）；携带T等位基因的母亲子代患NSCL／P的风险为未携带T等位基因的母亲子代的2．243倍（95％CI：1．408~3．572）。患者的父亲与正常儿童父亲的T等位基因频率分别为62．92％和55．47％；C等位基因频率分别为37．08％和44．53％，其构成比差异无统计学意义（Y。=2．222，P〉0．05）；病例组和对照组后代可能为纯合突变胎儿的机率分别为43％和29％（P〉0．05）。结论山东地区母亲的MTHFR基因677C／T突变对后代NSCL／P的发生有重要的影响；父亲的MTHFR基因677C／T突变则可能不是子代患NSCL／P的风险因素。
