The ATP in roots and xylem sap of two woody plant species, Platycladus orientalis and Acacia auriculiformis, subjected to rapid and slow soil drying has been determined employing firefly luciferase ATP assay method (s...The ATP in roots and xylem sap of two woody plant species, Platycladus orientalis and Acacia auriculiformis, subjected to rapid and slow soil drying has been determined employing firefly luciferase ATP assay method (sensitivity is at 10<sup>-12</sup> mol ATP L<sup>-1</sup>). The ATP levels in the two species were 1.6 nmol. g<sub>DW</sub><sup>-1</sup> and 0.6 nmol. g<sub>DW</sub><sup>-1</sup> in roots, and 5.6 μmol·m<sup>-3</sup> and 8 μmol ·m<sup>-3</sup> in xylem sap, respectively. When plants of P. orientalis and A. auriculiformis were subjected to rapid soil drying, respectively, as soil water content (SWC) decreased from the normal level ( 0.2.5 g·g<sub>DW</sub><sup>-1</sup>) to 0.02 and 0.06 g·g<sub>DW</sub><sup>-1</sup>, separately, plant water potential ( ψ )dropped to - 4 and - 3.2 MPa, differently, the ATP in roots decreased 99.7% and 42%, respectively. When the rapidly dried soil was watered for up to 6 d, SWC and ψ, were found to recover to their normal levels, but ATP content in roots of P. orientalis and A. auriculiformis recovered by 10% and 23%, respectively. When plants展开更多
文摘The ATP in roots and xylem sap of two woody plant species, Platycladus orientalis and Acacia auriculiformis, subjected to rapid and slow soil drying has been determined employing firefly luciferase ATP assay method (sensitivity is at 10<sup>-12</sup> mol ATP L<sup>-1</sup>). The ATP levels in the two species were 1.6 nmol. g<sub>DW</sub><sup>-1</sup> and 0.6 nmol. g<sub>DW</sub><sup>-1</sup> in roots, and 5.6 μmol·m<sup>-3</sup> and 8 μmol ·m<sup>-3</sup> in xylem sap, respectively. When plants of P. orientalis and A. auriculiformis were subjected to rapid soil drying, respectively, as soil water content (SWC) decreased from the normal level ( 0.2.5 g·g<sub>DW</sub><sup>-1</sup>) to 0.02 and 0.06 g·g<sub>DW</sub><sup>-1</sup>, separately, plant water potential ( ψ )dropped to - 4 and - 3.2 MPa, differently, the ATP in roots decreased 99.7% and 42%, respectively. When the rapidly dried soil was watered for up to 6 d, SWC and ψ, were found to recover to their normal levels, but ATP content in roots of P. orientalis and A. auriculiformis recovered by 10% and 23%, respectively. When plants