Blast,a disease caused by Magnaporthe oryzae,is a major constraint for rice production worldwide.Introgression of durable blast resistance genes into high-yielding rice cultivars has been considered a priority to cont...Blast,a disease caused by Magnaporthe oryzae,is a major constraint for rice production worldwide.Introgression of durable blast resistance genes into high-yielding rice cultivars has been considered a priority to control the disease.The blast resistance Pik locus,located on chromosome 11,contains at least six important resistance genes,but these genes have not been widely employed in resistance breeding since existing markers hardly satisfy current breeding needs due to their limited scope of application.In this study,two PCR-based markers,Pikp-Del and Pi1-In,were developed to target the specific In Del(insertion/deletion)of the Pik-p and Pi-1 genes,respectively.The two markers precisely distinguished Pik-p,Pi-1,and the K-type alleles at the Pik locus,which is a necessary element for functional genes from rice varieties.Results also revealed that only several old varieties contain the two genes,of which nearly half carry the K-type alleles.Therefore,these identified varieties can serve as new gene sources for developing blast resistant rice.The two newly developed markers will be highly useful for the use of Pik-p,Pi-1 and other resistance genes at the Pik locus in markerassisted selection(MAS)breeding programs.展开更多
Within the framework of the U<SUB>sdpf</SUB>(16) interacting boson model (IBM), the effects of strong correlations of the dipole (p<SUP>?</SUP>-boson) and the octupole (f<SUP>?</SUP>...Within the framework of the U<SUB>sdpf</SUB>(16) interacting boson model (IBM), the effects of strong correlations of the dipole (p<SUP>?</SUP>-boson) and the octupole (f<SUP>?</SUP>-boson) degree of freedom on the positive-parity states of even-even nuclei in SU(3) limit are discussed. It is shown that configurations of an even number of many p- and f-bosons can not only be incorporated into the usual low-lying collective rotational bands, such as the ground state band, β- and γ-vibrational bands, but also naturally form the rotational bands, etc. These results are similar to that of U<SUB>sdg</SUB>(15)-IBM and in good agreement with the experimental data of the nucleus. Besides, several intraband E2 transition probabilities are given, which are consistent with that of U<SUB>sd</SUB>(6)-IBM.展开更多
Fungi blast is one of the most serious diseases of rice worldwide. Breeding resistant varieties have been proved to be the most effective and economical means to control the disease. This paper describes the molecular...Fungi blast is one of the most serious diseases of rice worldwide. Breeding resistant varieties have been proved to be the most effective and economical means to control the disease. This paper describes the molecular marker-assisted selection (MAS) procedure for a broad-spectrum blast resistant gene Pi1 integrated into an elite hybrid maintainer line, Zhenshan 97. A simple sequence repeat (SSR) based on molecular marker-aided selection system for Pi1 segment was established. Using a backcross population and a blast isolate F1829, Pi1 gene was mapped on the top of chromosome 11 between markers RZ536 and RM144, with a distance of 9.7 cM and 6.8 cM, respectively. Seventeen families derived from the recurrent parent Zhenshan 97 were obtained with homozygous Pi1 gene. The background of the 17 families was identified with inter simple sequence repeat (ISSR) amplification, the highest recovery of the Zhenshan 97 genetic background was 97.01% after the assay of 167 polymorphic bands.展开更多
基金the National Natural Science Foundation of China(31640006)the China Postdocotral Science Foundation(2019M662219)+1 种基金the Youngth Program of Fujian Academy of Agricultural Sciences(YC2019004)the Projection of Public Welfare of Fujian Province(2017R1019-10)。
文摘Blast,a disease caused by Magnaporthe oryzae,is a major constraint for rice production worldwide.Introgression of durable blast resistance genes into high-yielding rice cultivars has been considered a priority to control the disease.The blast resistance Pik locus,located on chromosome 11,contains at least six important resistance genes,but these genes have not been widely employed in resistance breeding since existing markers hardly satisfy current breeding needs due to their limited scope of application.In this study,two PCR-based markers,Pikp-Del and Pi1-In,were developed to target the specific In Del(insertion/deletion)of the Pik-p and Pi-1 genes,respectively.The two markers precisely distinguished Pik-p,Pi-1,and the K-type alleles at the Pik locus,which is a necessary element for functional genes from rice varieties.Results also revealed that only several old varieties contain the two genes,of which nearly half carry the K-type alleles.Therefore,these identified varieties can serve as new gene sources for developing blast resistant rice.The two newly developed markers will be highly useful for the use of Pik-p,Pi-1 and other resistance genes at the Pik locus in markerassisted selection(MAS)breeding programs.
文摘Within the framework of the U<SUB>sdpf</SUB>(16) interacting boson model (IBM), the effects of strong correlations of the dipole (p<SUP>?</SUP>-boson) and the octupole (f<SUP>?</SUP>-boson) degree of freedom on the positive-parity states of even-even nuclei in SU(3) limit are discussed. It is shown that configurations of an even number of many p- and f-bosons can not only be incorporated into the usual low-lying collective rotational bands, such as the ground state band, β- and γ-vibrational bands, but also naturally form the rotational bands, etc. These results are similar to that of U<SUB>sdg</SUB>(15)-IBM and in good agreement with the experimental data of the nucleus. Besides, several intraband E2 transition probabilities are given, which are consistent with that of U<SUB>sd</SUB>(6)-IBM.
文摘Fungi blast is one of the most serious diseases of rice worldwide. Breeding resistant varieties have been proved to be the most effective and economical means to control the disease. This paper describes the molecular marker-assisted selection (MAS) procedure for a broad-spectrum blast resistant gene Pi1 integrated into an elite hybrid maintainer line, Zhenshan 97. A simple sequence repeat (SSR) based on molecular marker-aided selection system for Pi1 segment was established. Using a backcross population and a blast isolate F1829, Pi1 gene was mapped on the top of chromosome 11 between markers RZ536 and RM144, with a distance of 9.7 cM and 6.8 cM, respectively. Seventeen families derived from the recurrent parent Zhenshan 97 were obtained with homozygous Pi1 gene. The background of the 17 families was identified with inter simple sequence repeat (ISSR) amplification, the highest recovery of the Zhenshan 97 genetic background was 97.01% after the assay of 167 polymorphic bands.
