Objective: The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells ...Objective: The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells (LECs) is a primary goal in preventing PCO. Here, we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) cells. Methods: Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 20 mg/L Cur in a CO2 incubator for 24 h. Results: We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control group. Furthermore, the A value of the Cur group was significantly lower com- pared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF. Eight different protein spots were obtained in HLE-B3 cells incubated with Cur. There were the common variational protein spots at mass/charge (m/z) ratios of 8 093 and 13 767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group. Conclusions: These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. The protein spots at m/z of 8 093 and 13 767 may be the targets of Cur-induced inhibition of HLE-B3 cell proliferation. Cur may be a reliable and effective drug for pre- vention and treatment of polymerase chain reaction (PCR).展开更多
针对状态空间规模较大的协议,提出了一种基于着色Petri网CPN(colored petri net)、面向属性的协议一致性测试序列生成方法。首先,构建被测协议规范的CPN模型,即协议模型,并将被测协议的若干功能属性采用CPN进行形式描述,形成属性模型。...针对状态空间规模较大的协议,提出了一种基于着色Petri网CPN(colored petri net)、面向属性的协议一致性测试序列生成方法。首先,构建被测协议规范的CPN模型,即协议模型,并将被测协议的若干功能属性采用CPN进行形式描述,形成属性模型。提出了属性模型到协议模型的状态投影算法、模型状态空间的路径匹配算法和搜索算法,基于这些算法给出了一种完整的基于属性的测试序列生成方法。该方法不但可以有效避免协议规模庞大所导致的状态爆炸问题,而且还对测试中的PCO库所进行了特殊标识,完成测试序列中可观察元素的自动提取处理,使得处理后的测试序列可直接用于协议实际测试过程。为有效验证所提测试序列生成方法的可行性,开发了一套实现该方法的软件,并应用于HMIPv6协议的实际测试过程中。实践表明,这套软件可以对较为复杂的协议一致性测试过程提供强有力的支持。展开更多
基金Project(No.wzzb0605) supported by the Traditional Chinese Medicine Research Fund of the Department of Health of Fujian Province,China
文摘Objective: The incidence of after-cataracts [also known as posterior capsular opacification (PCO)] is between 30% and 50% three years following cataract surgery. Suppressing the proliferation of lens epithelial cells (LECs) is a primary goal in preventing PCO. Here, we investigated the proteomic regulation of the inhibitory effects of curcumin (Cur) on the proliferation of human lens epithelial B3 (HLE-B3) cells. Methods: Recombinant human basic fibroblast growth factor (rhbFGF) was used to induce proliferation of HLE-B3 cells, which were incubated with 20 mg/L Cur in a CO2 incubator for 24 h. Results: We found that the absorbance (A) value of rhbFGF group was significantly higher than the A value of the control group. Furthermore, the A value of the Cur group was significantly lower com- pared to the rhbFGF group, with an inhibition of 53.7%. Five different protein spots were obtained from proliferative HLE-B3 cells induced by rhbFGF. Eight different protein spots were obtained in HLE-B3 cells incubated with Cur. There were the common variational protein spots at mass/charge (m/z) ratios of 8 093 and 13 767 between rhbFGF group and control group as well as between the Cur group and rhbFGF group. Conclusions: These results show that Cur effectively inhibited HLE-B3 cell proliferation induced by rhbFGF. The protein spots at m/z of 8 093 and 13 767 may be the targets of Cur-induced inhibition of HLE-B3 cell proliferation. Cur may be a reliable and effective drug for pre- vention and treatment of polymerase chain reaction (PCR).
文摘针对状态空间规模较大的协议,提出了一种基于着色Petri网CPN(colored petri net)、面向属性的协议一致性测试序列生成方法。首先,构建被测协议规范的CPN模型,即协议模型,并将被测协议的若干功能属性采用CPN进行形式描述,形成属性模型。提出了属性模型到协议模型的状态投影算法、模型状态空间的路径匹配算法和搜索算法,基于这些算法给出了一种完整的基于属性的测试序列生成方法。该方法不但可以有效避免协议规模庞大所导致的状态爆炸问题,而且还对测试中的PCO库所进行了特殊标识,完成测试序列中可观察元素的自动提取处理,使得处理后的测试序列可直接用于协议实际测试过程。为有效验证所提测试序列生成方法的可行性,开发了一套实现该方法的软件,并应用于HMIPv6协议的实际测试过程中。实践表明,这套软件可以对较为复杂的协议一致性测试过程提供强有力的支持。
