目的:分析腹腔镜辅助下胆总管探查术及胆囊切除术治疗胆囊结石合并胆总管结石患者的疗效。方法:2015年1月—2017年8月,122例胆囊结石合并胆总管结石患者随机分为两组,对照组(61例)实施腹腔镜辅助下胆囊切除术(LC)联合内镜括约肌切开术(E...目的:分析腹腔镜辅助下胆总管探查术及胆囊切除术治疗胆囊结石合并胆总管结石患者的疗效。方法:2015年1月—2017年8月,122例胆囊结石合并胆总管结石患者随机分为两组,对照组(61例)实施腹腔镜辅助下胆囊切除术(LC)联合内镜括约肌切开术(EST),观察组(61例)实施腹腔镜辅助下胆总管探查术(LCBDE)及胆囊切除术(LC)察组患者根据术毕时刻腹腔内气体处理方法不同分为氧气置换(OR)患者与未置换(NOR)患者对比两组结石清除率、手术时间、术中出血量、肛门排气时间、住院时间、住院费用、淀粉酶(AMY)、C反应蛋白(CRP)水平、并发症发生情况及术后1年复发率,并对比OR患者与NOR患者术后24 h、72 h白细胞计数(WBC)、降钙素原(PCT)、中性粒细胞百分比(GRA)。结果:观察组结石清除率(98.37%)与对照组(95.08%)差异无统计学意义(P>0.05);观察组手术时间、肛门排气时间、住院时间短于对照组,术中出血量、住院费用低于对照组(P<0.05);观察组术后24 h、72 h AMY、CRP低于对照组(P<0.05);OR患者术后24 h、72 h WBC、PCT、GRA水平低于NOR患者(P<0.05);观察组并发症发生率(3.28%)低于对照组(13.11%,P<0.05);观察组复发率(1.64%)与对照组(3.28%)无显著差异(P>0.05)。结论:腹腔镜辅助下胆总管探查术及胆囊切除术治疗胆囊结石合并胆总管结石具有较高结石清除率,能缩短手术时间,减少术中出血量,减轻对胆道与胰腺损伤,加快患者恢复,减轻机体应激,降低并发症发生率且术毕时刻应用氧气置换腹腔镜手术后残留二氧化碳能创造抑菌环境,有助于改善术后感染情况。展开更多
Mitochondria are essential cellular organelles critical for generating adenosine triphosphate for cellular homeostasis, as well as various mechanisms that can lead to both necrosis and apoptosis. The field of ＂mi- to...Mitochondria are essential cellular organelles critical for generating adenosine triphosphate for cellular homeostasis, as well as various mechanisms that can lead to both necrosis and apoptosis. The field of ＂mi- tochondrial medicine＂ is emerging in which injury/disease states are targeted therapeutically at the level of the mitochondrion, including specific antioxidants, bioenergetic substrate additions, and membrane uncoupling agents. Consequently, novel mitochondrial transplantation strategies represent a potentially multifactorial therapy leading to increased adenosine triphosphate production, decreased oxidative stress, mitochondrial DNA replacement, improved bioenergetics and tissue sparing. Herein, we describe briefly the history of mitochondrial transplantation and the various techniques used for both in vitro and in vivo delivery, the benefits associated with successful transference into both peripheral and central nervous system tissues, along with caveats and pitfalls that hinder the advancements of this novel therapeutic.展开更多
目的研究心脏瓣膜置换术后早期认知功能障碍(P0CD)的发生与术中局部脑组织氧饱和度变化的关系。方法选取择期行心脏瓣膜置换术的患者82例,入手术室后监测脑氧饱和度(rSO2),记录基线值(rSO2base)、最小值(rSO2min)、相比基线降低的最大...目的研究心脏瓣膜置换术后早期认知功能障碍(P0CD)的发生与术中局部脑组织氧饱和度变化的关系。方法选取择期行心脏瓣膜置换术的患者82例,入手术室后监测脑氧饱和度(rSO2),记录基线值(rSO2base)、最小值(rSO2min)、相比基线降低的最大百分数(rSO2%max)和低于基线值20%的阈值下面积(AUT2O%)。分别于术前1d和术后7d采用神经心理学测试的方法评估患者的认知功能,根据评估结果分为POCD组和non-POCD组,比较2组rS02的差异。结果POCD组34例,non-POCD组48例,P0 C D发生率41.5%。POCD组rSO2%max、AUT20%均高于non-P0CD组,差异有统计学意义(P<0.05)。结论心脏瓣膜置换术后早期认知功能障碍的发生与术中局部脑组织氧饱和度的降低有关,术中尽量减少rS02低于基线值20%以下的暴露时间可能有助于减少POCD的发生。展开更多
文摘目的:分析腹腔镜辅助下胆总管探查术及胆囊切除术治疗胆囊结石合并胆总管结石患者的疗效。方法:2015年1月—2017年8月,122例胆囊结石合并胆总管结石患者随机分为两组,对照组(61例)实施腹腔镜辅助下胆囊切除术(LC)联合内镜括约肌切开术(EST),观察组(61例)实施腹腔镜辅助下胆总管探查术(LCBDE)及胆囊切除术(LC)察组患者根据术毕时刻腹腔内气体处理方法不同分为氧气置换(OR)患者与未置换(NOR)患者对比两组结石清除率、手术时间、术中出血量、肛门排气时间、住院时间、住院费用、淀粉酶(AMY)、C反应蛋白(CRP)水平、并发症发生情况及术后1年复发率,并对比OR患者与NOR患者术后24 h、72 h白细胞计数(WBC)、降钙素原(PCT)、中性粒细胞百分比(GRA)。结果:观察组结石清除率(98.37%)与对照组(95.08%)差异无统计学意义(P>0.05);观察组手术时间、肛门排气时间、住院时间短于对照组,术中出血量、住院费用低于对照组(P<0.05);观察组术后24 h、72 h AMY、CRP低于对照组(P<0.05);OR患者术后24 h、72 h WBC、PCT、GRA水平低于NOR患者(P<0.05);观察组并发症发生率(3.28%)低于对照组(13.11%,P<0.05);观察组复发率(1.64%)与对照组(3.28%)无显著差异(P>0.05)。结论:腹腔镜辅助下胆总管探查术及胆囊切除术治疗胆囊结石合并胆总管结石具有较高结石清除率,能缩短手术时间,减少术中出血量,减轻对胆道与胰腺损伤,加快患者恢复,减轻机体应激,降低并发症发生率且术毕时刻应用氧气置换腹腔镜手术后残留二氧化碳能创造抑菌环境,有助于改善术后感染情况。
基金funded by NIH R21NS096670(AGR)University of Kentucky Spinal Cord and Brain Injury Research Center Chair Endowment(AGR),NIH/NINDS 2P30NS051220
文摘Mitochondria are essential cellular organelles critical for generating adenosine triphosphate for cellular homeostasis, as well as various mechanisms that can lead to both necrosis and apoptosis. The field of ＂mi- tochondrial medicine＂ is emerging in which injury/disease states are targeted therapeutically at the level of the mitochondrion, including specific antioxidants, bioenergetic substrate additions, and membrane uncoupling agents. Consequently, novel mitochondrial transplantation strategies represent a potentially multifactorial therapy leading to increased adenosine triphosphate production, decreased oxidative stress, mitochondrial DNA replacement, improved bioenergetics and tissue sparing. Herein, we describe briefly the history of mitochondrial transplantation and the various techniques used for both in vitro and in vivo delivery, the benefits associated with successful transference into both peripheral and central nervous system tissues, along with caveats and pitfalls that hinder the advancements of this novel therapeutic.
基金supported by the Special Foundation for High-level Radioactive Waste Disposal(2007-840,2012-851)the National Natural Science Foundation of China(U1530112,11475008,11075006,91026010)
文摘目的研究心脏瓣膜置换术后早期认知功能障碍(P0CD)的发生与术中局部脑组织氧饱和度变化的关系。方法选取择期行心脏瓣膜置换术的患者82例,入手术室后监测脑氧饱和度(rSO2),记录基线值(rSO2base)、最小值(rSO2min)、相比基线降低的最大百分数(rSO2%max)和低于基线值20%的阈值下面积(AUT2O%)。分别于术前1d和术后7d采用神经心理学测试的方法评估患者的认知功能,根据评估结果分为POCD组和non-POCD组,比较2组rS02的差异。结果POCD组34例,non-POCD组48例,P0 C D发生率41.5%。POCD组rSO2%max、AUT20%均高于non-P0CD组,差异有统计学意义(P<0.05)。结论心脏瓣膜置换术后早期认知功能障碍的发生与术中局部脑组织氧饱和度的降低有关,术中尽量减少rS02低于基线值20%以下的暴露时间可能有助于减少POCD的发生。
