本文用磷酸铅沉淀技术对金鱼草(Antirrhinum majus L.)开花前2天和开花当天胚珠中 ATP 酶的超微细胞化学进行了研究。卵器没有酶活反应;中央细胞的质膜、细胞质和核质中均有酶活反应。胚囊壁与其外方角质层之间有一间隙层,其中含有一些...本文用磷酸铅沉淀技术对金鱼草(Antirrhinum majus L.)开花前2天和开花当天胚珠中 ATP 酶的超微细胞化学进行了研究。卵器没有酶活反应;中央细胞的质膜、细胞质和核质中均有酶活反应。胚囊壁与其外方角质层之间有一间隙层,其中含有一些絮状和小囊泡状物质,该层呈现强烈的酶活反应。合点端承珠盘组织的细胞核、质膜及其特殊的厚壁内有大量的酶,并通过合点端角质层的不连续区段与上述间隙层中的酶连成一片。一般珠被薄壁细胞仅质膜和胞间连丝上有酶活反应,但珠被绒毡层细胞核质中相当显著。根据 ATP 酶在胚珠中的分布特点,着重讨论了珠被绒毡层和承珠盘的功能,并推测胚囊壁外间隙层可能构成一个为胚囊提供养料的质外体运输系统。展开更多
Ovule and seed developments are crucial processes during plant growth, which are affected by different signaling pathways. In this paper, we demonstrate that the brassinosteroid （BR） signal is involved in ovule init...Ovule and seed developments are crucial processes during plant growth, which are affected by different signaling pathways. In this paper, we demonstrate that the brassinosteroid （BR） signal is involved in ovule initiation and development. Ovule and seed numbers are significantly different when comparing BR-related mutants to wild-type con-trols. Detailed observation indicates that BR regulates the expression level of genes related to ovule development, includ-ing HLL, ANT, and AP2, either directly by targeting the promoter sequences or indirectly via regulation by BR-induced transcription factor BZR1. Also, Western blot demonstrates that the dephosphorylation level of BZR1 is consistent with ovule and seed number. The intragenic bzrl-lD suppressors bzs247 and bzs248 have much fewer ovules and seeds than bzrl-lD, which are similar to wild-type, suggesting that the phenotype can be rescued. The molecular and genetic experi-ments confirm that BZR1 and AP2 probably affect Arabidopsis ovule number determination antagonistically.展开更多
文摘本文用磷酸铅沉淀技术对金鱼草(Antirrhinum majus L.)开花前2天和开花当天胚珠中 ATP 酶的超微细胞化学进行了研究。卵器没有酶活反应;中央细胞的质膜、细胞质和核质中均有酶活反应。胚囊壁与其外方角质层之间有一间隙层,其中含有一些絮状和小囊泡状物质,该层呈现强烈的酶活反应。合点端承珠盘组织的细胞核、质膜及其特殊的厚壁内有大量的酶,并通过合点端角质层的不连续区段与上述间隙层中的酶连成一片。一般珠被薄壁细胞仅质膜和胞间连丝上有酶活反应,但珠被绒毡层细胞核质中相当显著。根据 ATP 酶在胚珠中的分布特点,着重讨论了珠被绒毡层和承珠盘的功能,并推测胚囊壁外间隙层可能构成一个为胚囊提供养料的质外体运输系统。
文摘Ovule and seed developments are crucial processes during plant growth, which are affected by different signaling pathways. In this paper, we demonstrate that the brassinosteroid （BR） signal is involved in ovule initiation and development. Ovule and seed numbers are significantly different when comparing BR-related mutants to wild-type con-trols. Detailed observation indicates that BR regulates the expression level of genes related to ovule development, includ-ing HLL, ANT, and AP2, either directly by targeting the promoter sequences or indirectly via regulation by BR-induced transcription factor BZR1. Also, Western blot demonstrates that the dephosphorylation level of BZR1 is consistent with ovule and seed number. The intragenic bzrl-lD suppressors bzs247 and bzs248 have much fewer ovules and seeds than bzrl-lD, which are similar to wild-type, suggesting that the phenotype can be rescued. The molecular and genetic experi-ments confirm that BZR1 and AP2 probably affect Arabidopsis ovule number determination antagonistically.
