Salvianolic acid G,a caffeic acid dimer with a novel tetracyclic skeleton was isolated from the roots of Salvia miltiorrhiza.Its structure was elucidated by chemical and spectral analysis,especially by 2D NMR analysis.
A new depside named salvianolic acid H was isolated from the aqueous extract of Salvia cavaleriei vat.simplicifolia,along with salvianolic acid A,B,C,isosalvianolic acid C,lithospermic acid, rosmarinic acid,R-(+)-β-(...A new depside named salvianolic acid H was isolated from the aqueous extract of Salvia cavaleriei vat.simplicifolia,along with salvianolic acid A,B,C,isosalvianolic acid C,lithospermic acid, rosmarinic acid,R-(+)-β-(3,4-dihydroxyphenyl)lacticacid and protocatechualdehyde.Rosmarinic acid was obtained as the major component.展开更多
目的探讨骨髓中自然杀伤T细胞(nature killer T cell,NKT)在再生障碍性贫血免疫介导发病机制中的作用。方法选取14例确诊儿童再生障碍性贫血为再生障碍性贫血组,同年龄正常儿童10例为对照组。流式细胞术检测两组儿童骨髓中NKT含量;利用...目的探讨骨髓中自然杀伤T细胞(nature killer T cell,NKT)在再生障碍性贫血免疫介导发病机制中的作用。方法选取14例确诊儿童再生障碍性贫血为再生障碍性贫血组,同年龄正常儿童10例为对照组。流式细胞术检测两组儿童骨髓中NKT含量;利用免疫磁珠法分离纯化NKT细胞后,分别在4种不同体系下进行扩增培养。A:α-Galcer+rhIL-2;B:α-Galcer+rhIL-2+rhG-CSF;C:OCH+rhIL-2;D:OCH+rhIL-2+rhG-CSF。测定NKT细胞在不同培养条件下的扩增倍数;利用酶联免疫斑点技术(Elispot)测定NKT细胞扩增活化后表达IFN-γ、IL-4的斑点形成细胞数(SFCs)。结果再生障碍性贫血组骨髓中NKT百分率(0.827%±0.022%)明显低于对照组(1.033%±0.073%,Z=-3.810,P=0.000)。在各培养体系中,再生障碍性贫血组和对照组骨髓NKT均可明显扩增。在含有α-Galcer培养体系A和B中,再生障碍性贫血组及对照组NKT细胞的扩增能力均高于OCH体系C和D,差异有统计学意义(P<0.01或P<0.05)。在培养体系中加入rhG-CSF后,再生障碍性贫血组骨髓NKT细胞的扩增能力下降(P<0.01),但表达IFN-γ的SFCs明显减少(P均<0.01);而表达IL-4的SFCs明显升高(P均<0.01)。结论再生障碍性贫血患儿骨髓NKT的数量和功能均明显低于正常儿童。但再生障碍性贫血骨髓在接受细胞配体(OCH或α-Galcer)+rhIL-2+rhG-CSF作用后,在获得NKT细胞一定程度扩增的同时,提高NKT的IL-4表达,降低NKT的IFN-γ表达,抑制T细胞向Th1分化,而促进T细胞向Th2分化,逆转再生障碍性贫血Th1/Th2异常失衡的免疫介导致病机制,可能成为获得性再生障碍性贫血的有效治疗途径。展开更多
(CH_3OCH_2CH_2C_5H_4)_2LnI(Ln=La,Nd)complexes have been synthesized by the reaction between NaI and[(CH_3OCH_2CH_2C_5H_4)_2LnCl]2 in THF;A single crystal X-ray study has shown that the lanthanum complex is an unsolvat...(CH_3OCH_2CH_2C_5H_4)_2LnI(Ln=La,Nd)complexes have been synthesized by the reaction between NaI and[(CH_3OCH_2CH_2C_5H_4)_2LnCl]2 in THF;A single crystal X-ray study has shown that the lanthanum complex is an unsolvated monomer with lanthanum(Ⅲ)in a trigonal bipyramidal coordination geometry if the cyclopentadienyl ring is regarded as occupying a single polyhedral vertex.展开更多
Kadsurenin M (7s, 8s- 3, 4, 3'- trimethoxy- 7'- o\o- nor-8', 9'-7. O, 4' - 8, 5'-neolingan)(1), a benzofuranoid neolignan isolated tyom the aerial part of Piper Kadsura (Choisy) Ohwi,was synthe...Kadsurenin M (7s, 8s- 3, 4, 3'- trimethoxy- 7'- o\o- nor-8', 9'-7. O, 4' - 8, 5'-neolingan)(1), a benzofuranoid neolignan isolated tyom the aerial part of Piper Kadsura (Choisy) Ohwi,was synthesized in 6 steps from vanillin via a key intermediate 3,4- dimethoxycinnamyl (2'-methoxy- 4'- formyl)phenyl ether (7). The overall yield is 15%.展开更多
The first synthesis of squarnosamide,a new compound isolated from Annona squamosa, was achieved in seven steps.The over all yield was 14%.The compound was identified by^1 H,^(13)C NMR and MS.
Five new zwitterionic surfactants with long chain alkyl betaine structure incorporated with hydroxylpropyl group have been synthesized. Their structures were identified by elemental analysis, IR (HNMR)-H-1, and (CNMR)...Five new zwitterionic surfactants with long chain alkyl betaine structure incorporated with hydroxylpropyl group have been synthesized. Their structures were identified by elemental analysis, IR (HNMR)-H-1, and (CNMR)-C-13. Surface tension experiments showed that these surfactants have higher surface activity than those without hydroxypropyl group. The values of CMC and gamma(CMC) of these surfactants have been determined.展开更多
Nine compounds were isolated from the acetone soluble fraction of ethanolic extract of the roots of Belamcanda chinensis (L.)DC. The structure of new compound I,named shegansu A,has been established as irigenin-5-O-(6...Nine compounds were isolated from the acetone soluble fraction of ethanolic extract of the roots of Belamcanda chinensis (L.)DC. The structure of new compound I,named shegansu A,has been established as irigenin-5-O-(6'-O-vanillin acid)-β-D-glucoside(Ⅰ) by the use of spectroscopic evidence (UV, IR. FAB-MS.1HNMR, 13CNMR,1H-13CNMR and NOE difference spectrum) and chemical methods.The well known compounds were identified as isorhapotigenin (Ⅲ),resveratrol(Ⅳ), P-hydroxy benzoic acid (Ⅴ), iridin (Ⅵ), tectoridin (Ⅶ),tectorigenin(Ⅷ) and daucosterol(Ⅸ). Ⅲ-Ⅸ were isolated from the plant for the first time.展开更多
文摘Salvianolic acid G,a caffeic acid dimer with a novel tetracyclic skeleton was isolated from the roots of Salvia miltiorrhiza.Its structure was elucidated by chemical and spectral analysis,especially by 2D NMR analysis.
文摘A new depside named salvianolic acid H was isolated from the aqueous extract of Salvia cavaleriei vat.simplicifolia,along with salvianolic acid A,B,C,isosalvianolic acid C,lithospermic acid, rosmarinic acid,R-(+)-β-(3,4-dihydroxyphenyl)lacticacid and protocatechualdehyde.Rosmarinic acid was obtained as the major component.
文摘目的探讨骨髓中自然杀伤T细胞(nature killer T cell,NKT)在再生障碍性贫血免疫介导发病机制中的作用。方法选取14例确诊儿童再生障碍性贫血为再生障碍性贫血组,同年龄正常儿童10例为对照组。流式细胞术检测两组儿童骨髓中NKT含量;利用免疫磁珠法分离纯化NKT细胞后,分别在4种不同体系下进行扩增培养。A:α-Galcer+rhIL-2;B:α-Galcer+rhIL-2+rhG-CSF;C:OCH+rhIL-2;D:OCH+rhIL-2+rhG-CSF。测定NKT细胞在不同培养条件下的扩增倍数;利用酶联免疫斑点技术(Elispot)测定NKT细胞扩增活化后表达IFN-γ、IL-4的斑点形成细胞数(SFCs)。结果再生障碍性贫血组骨髓中NKT百分率(0.827%±0.022%)明显低于对照组(1.033%±0.073%,Z=-3.810,P=0.000)。在各培养体系中,再生障碍性贫血组和对照组骨髓NKT均可明显扩增。在含有α-Galcer培养体系A和B中,再生障碍性贫血组及对照组NKT细胞的扩增能力均高于OCH体系C和D,差异有统计学意义(P<0.01或P<0.05)。在培养体系中加入rhG-CSF后,再生障碍性贫血组骨髓NKT细胞的扩增能力下降(P<0.01),但表达IFN-γ的SFCs明显减少(P均<0.01);而表达IL-4的SFCs明显升高(P均<0.01)。结论再生障碍性贫血患儿骨髓NKT的数量和功能均明显低于正常儿童。但再生障碍性贫血骨髓在接受细胞配体(OCH或α-Galcer)+rhIL-2+rhG-CSF作用后,在获得NKT细胞一定程度扩增的同时,提高NKT的IL-4表达,降低NKT的IFN-γ表达,抑制T细胞向Th1分化,而促进T细胞向Th2分化,逆转再生障碍性贫血Th1/Th2异常失衡的免疫介导致病机制,可能成为获得性再生障碍性贫血的有效治疗途径。
文摘(CH_3OCH_2CH_2C_5H_4)_2LnI(Ln=La,Nd)complexes have been synthesized by the reaction between NaI and[(CH_3OCH_2CH_2C_5H_4)_2LnCl]2 in THF;A single crystal X-ray study has shown that the lanthanum complex is an unsolvated monomer with lanthanum(Ⅲ)in a trigonal bipyramidal coordination geometry if the cyclopentadienyl ring is regarded as occupying a single polyhedral vertex.
文摘Kadsurenin M (7s, 8s- 3, 4, 3'- trimethoxy- 7'- o\o- nor-8', 9'-7. O, 4' - 8, 5'-neolingan)(1), a benzofuranoid neolignan isolated tyom the aerial part of Piper Kadsura (Choisy) Ohwi,was synthesized in 6 steps from vanillin via a key intermediate 3,4- dimethoxycinnamyl (2'-methoxy- 4'- formyl)phenyl ether (7). The overall yield is 15%.
文摘The first synthesis of squarnosamide,a new compound isolated from Annona squamosa, was achieved in seven steps.The over all yield was 14%.The compound was identified by^1 H,^(13)C NMR and MS.
文摘Five new zwitterionic surfactants with long chain alkyl betaine structure incorporated with hydroxylpropyl group have been synthesized. Their structures were identified by elemental analysis, IR (HNMR)-H-1, and (CNMR)-C-13. Surface tension experiments showed that these surfactants have higher surface activity than those without hydroxypropyl group. The values of CMC and gamma(CMC) of these surfactants have been determined.
文摘Nine compounds were isolated from the acetone soluble fraction of ethanolic extract of the roots of Belamcanda chinensis (L.)DC. The structure of new compound I,named shegansu A,has been established as irigenin-5-O-(6'-O-vanillin acid)-β-D-glucoside(Ⅰ) by the use of spectroscopic evidence (UV, IR. FAB-MS.1HNMR, 13CNMR,1H-13CNMR and NOE difference spectrum) and chemical methods.The well known compounds were identified as isorhapotigenin (Ⅲ),resveratrol(Ⅳ), P-hydroxy benzoic acid (Ⅴ), iridin (Ⅵ), tectoridin (Ⅶ),tectorigenin(Ⅷ) and daucosterol(Ⅸ). Ⅲ-Ⅸ were isolated from the plant for the first time.
