Nonalcoholic fatty liver disease(NAFLD) is well described as a common cause of chronic liver disease, mostly in the obese population. It refers to a spectrum of chronic liver disease that starts with simple steatosis ...Nonalcoholic fatty liver disease(NAFLD) is well described as a common cause of chronic liver disease, mostly in the obese population. It refers to a spectrum of chronic liver disease that starts with simple steatosis than progresses to nonalcoholic steatohepatitis and cirrhosis in patients without significant alcohol consumption. NAFLD in the non-obese population has been increasingly reported and studied recently. The pathogenesis of nonobese NAFLD is poorly understood and is related to genetic predisposition, most notably patatin-like phospholipase domain-containing 33 G allele polymorphism that leads to intrahepatic triglyceride accumulation and insulin resistance. Nonobese NAFLD is associated with components of metabolic syndrome and, especially, visceral obesity which seems to be an important etiological factor in this group. Dietary factors and, specifically, a high fructose diet seem to play a role. Cardiovascular events remain the main cause of mortality and morbidity in NAFLD, including in the nonobese population. There is not enough data regarding treatment in non-obese NAFLD patients, but similar to NAFLD in obese subjects, lifestyle changes that include dietary modification, physical activity, and weight loss remain the mainstay of treatment.展开更多
BACKGROUND Nonalcoholic fatty liver disease(NAFLD)has become one of the most common chronic liver diseases in the world.In our early clinical data and questionnaire analysis of NAFLD,it was found that the body mass in...BACKGROUND Nonalcoholic fatty liver disease(NAFLD)has become one of the most common chronic liver diseases in the world.In our early clinical data and questionnaire analysis of NAFLD,it was found that the body mass index of some patients did not meet the diagnostic criteria for overweight or obesity.The consumption of high-temperature-processed foods such as fried food,hot pot and barbecue is closely related to the occurrence of nonobese NAFLD.Reducing the intake of this kind of food can reduce disease severity and improve prognosis.AIM To explore the untargeted metabolomics characteristics of nonobese nonalcoholic fatty liver disease in Sprague-Dawley rats induced by high-temperatureprocessed feed.METHODS Fifty-four male Sprague-Dawley rats were divided into three groups:The control group received a standard diet;the nonfried soybeans(NDFS)group received 60%NDFS and 40%basic feed and the dry-fried soybeans(DFS)group received 60%DFS and 40%basic feed.Six rats were sacrificed at week 4,8,and 12 in each group.The food intake,body weight,Lee’s index,liver index,serological index and hepatic histopathology were assessed.Untargeted metabolomics characteristics were used to analyze the changes in liver metabolites of rats at week 12.Correlations between metabolites and pathology scores between the DFS and control groups and between the DFS and NDFS groups were analyzed.We selected some of the metabolites,both within the pathway and outside of the pathway,to explain preliminarily the difference in liver pathology in the three groups of rats.RESULTS There were no statistically significant differences in the food intake,body weight,Lee's index or serological index between the DFS group and the control group(P>0.05).At week 8 and week 12,the steatosis scores in the DFS group were significantly higher than those in the other two groups(P<0.05).At week 12,the liver index of the DFS group was the lowest(NDFS group vs DFS group,P<0.05).The fibrosis score in the DFS group was significantly higher than those in the other two g展开更多
Nonalcoholic fatty liver disease(NAFLD)is the most common liver disease worldwide,with an estimated prevalence of 25% globally.NAFLD is closely associated with metabolic syndrome,which are both becoming increasingly m...Nonalcoholic fatty liver disease(NAFLD)is the most common liver disease worldwide,with an estimated prevalence of 25% globally.NAFLD is closely associated with metabolic syndrome,which are both becoming increasingly more common with increasing rates of insulin resistance,dyslipidemia,and hypertension.Although NAFLD is strongly associated with obesity,lean or nonobese NAFLD is a relatively new phenotype and occurs in patients without increased waist circumference and with or without visceral fat.Currently,there is limited literature comparing and illustrating the differences between lean/nonobese and obese NAFLD patients with regard to risk factors,pathophysiology,and clinical outcomes.In this review,we aim to define and further delineate different phenotypes of NAFLD and present a comprehensive review on the prevalence,incidence,risk factors,genetic predisposition,and pathophysiology.Furthermore,we discuss and compare the clinical outcomes,such as insulin resistance,dyslipidemia,hypertension,coronary artery disease,mortality,and progression to nonalcoholic steatohepatitis,among lean/nonobese and obese NAFLD patients.Finally,we summarize the most up to date current management of NAFLD,including lifestyle interventions,pharmacologic therapies,and surgical options.展开更多
Background Sjǒgren syndrome （SS） is a systematic autoimmune disease, on which traditional therapeutic agents show limited effect. More effective agents with longer-lasting and fewer side effects are needed in the c...Background Sjǒgren syndrome （SS） is a systematic autoimmune disease, on which traditional therapeutic agents show limited effect. More effective agents with longer-lasting and fewer side effects are needed in the clinic. The aim of this study was to investigate the effects of Ganoderma lucindum spores （GLS） on sialoadenitis of nonobese diabetic （NOD） mice. Methods Thirty-two female NOD mice were assigned randomly into 4 groups： low-dose GLS-treated （L-GLS） group and high-dose GLS-treated （H-GLS） group, a dexamethasone group, and a normal saline （NS） control group. Stimulated total saliva flow rate （STFR）, area of lymphocytic infiltration in submandibular glands and ratios of CD4^＋ and CD8^＋ T lymphocytes and B lymphocytes in peripheral blood as well as apoptosis of these subsets and serum IgG level were tested after 10 weeks of treatments. Differences among the groups were analyzed by one-way analysis of variance （ANOVA）, Student-Newman-Keuls Test （SNK） was used between each two groups and a P 〈0.05 was considered statistically significant. Results STFR of the high-dose GLS group increased significantly after a 10-week treatment compared with those of the NS control group （P 〈0.05）. The incidence of sialoadenitis in GLS-treated NOD mice groups showed no significant difference compared with the control group （P 〉0.05）, but the area of lymphocytic foci in both the H-GLS and L-GLS groups decreased significantly to 50% of the NS control group （P 〈0.05）; the ratio of CD4^＋/CD8^＋ T lymphocytes and apoptosis of B lymphocytes of NOD mice with sialoadenitis were less and apoptosis of CD4^＋ and CD8^＋ T lymphocytes were significantly increased compared with the control group （P 〈0.05）. After pretreatment with H-GLS before sialoadenitis onset, the ratio of CD4^＋/CD8^＋ T lymphocyte and the serum IgG levels of NOD mice decreased significantly （P 〈0.05）. Conclusions Pretreatment with H-GLS can relieve symptoms of sialoadenitis in NOD mice. GL展开更多
Background Sjogren syndrome （SS） is an autoimmune disorder characterized by chronic lymphocytic infiltration and decreased secretion in salivary glands. Apoptosis is one of the possible mechanisms involved in acinar...Background Sjogren syndrome （SS） is an autoimmune disorder characterized by chronic lymphocytic infiltration and decreased secretion in salivary glands. Apoptosis is one of the possible mechanisms involved in acinar epithelial destruction in SS. The role of apoptosis in the initiation and effect phase of sialoadenitis is still controversial. The aim of this study was to observe the roles of apoptosis-associated proteins and serum IgG levels in sialoadenitis progression in nonobese diabetic （NOD） mice. Methods 2-, 5-, 10-, 15-, 20-week female NOD and matched BALB/c control mice were selected. Saliva and tear flow rate were measured. Serum IgG level was tested by enzyme-linked immunosorbent assay （ELISA）. Number of lymphocyte foci （NLF） in submandibular glands （SMGs） was counted under routine hematoxylin/eosin-stained sections. Expression of Fas, Bcl-2 and procaspase3 proteins as well as apoptotic cells in the SMGs were detected by immunohistochemical staining and by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling （TUNEL） assay respectively. Results Decreased stimulated total flow rate （STFR） and lymphocyte foci in SMGs were first observed in the 10-week NOD group. STFR was negatively correlated with NLF （P〈0.05）. Serum IgG in NOD mice was significantly higher than that of the control group （P〈0.05） and showed a positive correlation with NLF （P〈0.05）. Fas expression in SMGs acinar cells in NOD mice increased with age and was significantly higher compared with that in the control group. Bcl-2 expression and procaspase3 expression in SMG acinar cells in each NOD group were lower compared with those of the age-matched control mice. Conclusion Abnormal expression of Fas and Bcl-2 in the SMGs and higher level of serum IgG may contribute to the initiation of sialoadenitis and cause the glandular destruction in NOD mice.展开更多
The granin glycoprotein family consists of nine acidic proteins;chromogranin A(CgA),chromogranin B(CgB),and secretogranin II–VIII.They are produced by a wide range of neuronal,neuroendocrine,and endocrine cells throu...The granin glycoprotein family consists of nine acidic proteins;chromogranin A(CgA),chromogranin B(CgB),and secretogranin II–VIII.They are produced by a wide range of neuronal,neuroendocrine,and endocrine cells throughout the human body.Their major intracellular function is to sort peptides and proteins into secretory granules,but their cleavage products also take part in the extracellular regulation of diverse biological processes.The contribution of granins to carbohydrate metabolism and diabetes mellitus is a recent research area.CgA is associated with glucose homeostasis and the progression of type 1 diabetes.WE-14,CgA10-19,and CgA43-52 are peptide derivates of CgA,and act as CD4+or CD8+autoantigens in type 1 diabetes,whereas pancreastatin(PST)and catestatin have regulatory effects in carbohydrate metabolism.Furthermore,PST is related to gestational and type 2 diabetes.CgB has a crucial role in physiological insulin secretion.Secretogranins II and III have angiogenic activity in diabetic retinopathy(DR),and are novel targets in recent DR studies.Ongoing studies are beginning to investigate the potential use of granin derivatives as drugs to treat diabetes based on the divergent relationships between granins and different types of diabetes.展开更多
Prehypertension and hypertension have been considered significant public health issues worldwide[1].Disproportionate body fat distribution(excess fat stored in the liver and visceral compartment)is a well-established ...Prehypertension and hypertension have been considered significant public health issues worldwide[1].Disproportionate body fat distribution(excess fat stored in the liver and visceral compartment)is a well-established risk factor for prehypertension/hypertension[2].However,the most commonly used anthropometric indicators,such as body mass index(BMI)and waist circumference(WC)。展开更多
Objective:The objective of this study was to observe the effect of Bushen Qingre Yuyin Decoction on the spleen index,submandibular gland index,salivary secretion,submandibular gland morphology,and aquaporin 5(AQP5)exp...Objective:The objective of this study was to observe the effect of Bushen Qingre Yuyin Decoction on the spleen index,submandibular gland index,salivary secretion,submandibular gland morphology,and aquaporin 5(AQP5)expression levels in the submandibular gland.Materials and Methods:Fifty 8-week-old nonobese diabetic(NOD)female mice were randomly divided into the following five groups:model;hydroxychloroquine;and high-,medium-,and low-dose Bushen Qingre Yuyin Decoction prescription.The blank group consisted of ten 8-week-old Balb/C rats.Bushen Yuyin Qingre Decoction was administered by gavage at 20,40,and 80 g/kg in the low-,medium-,and high-dose groups,respectively.The hydroxychloroquine group was administered hydroxychloroquine at a dose of 80 mg/kg by gavage.The normal and model groups were administered the same amount of saline.After the different administrations,the amount of salivary secretion in the mice was regularly observed.After 12 weeks,the mice were sacrificed,and their submandibular gland tissues were excised,stained with hematoxylin-eosin,and pathologically scored.AQP5 expression levels in the tissues were detected using real-time polymerase chain reaction.Results:The spleen index of mice in the high-dose group was higher than that in the model group(P<0.05),and the submandibular gland index of the hydroxychloroquine group and all the Bushen Qingre Yuyin Decoction prescription groups was higher than that in the model group(P<0.05).After 6 weeks,the salivary volume of the hydroxychloroquine group and all the Bushen Qingre Yuyin Decoction prescription groups was significantly higher than that of the model group(P<0.05);the amount of lymphocyte infiltration in mice among each administration group was significantly reduced(P<0.05);and the AQP5 expression levels in the submandibular glands of mice had significantly increased in the hydroxychloroquine and high-dose groups(P<0.05),with the high-dose group showing the best effect.Conclusion:Bushen Qingre Yuyin Decoction can effectively increase the spleen and展开更多
目的观察麦冬地芍汤对干燥综合征模型(nonobese diabetic mouse,NOD)小鼠颌下腺的保护作用。方法取雌性NOD小鼠33只,随机分为模型组、羟氯喹组、麦冬地芍汤组,另取ICR(Institute of Cancer Research)小鼠8只作为空白组。适应性喂养一周...目的观察麦冬地芍汤对干燥综合征模型(nonobese diabetic mouse,NOD)小鼠颌下腺的保护作用。方法取雌性NOD小鼠33只,随机分为模型组、羟氯喹组、麦冬地芍汤组,另取ICR(Institute of Cancer Research)小鼠8只作为空白组。适应性喂养一周后,羟氯喹组、麦冬地芍汤组分别灌服羟氯喹溶液(0.1 g/kg,20 ml/kg)及麦冬地芍汤(10 g/kg,20 ml/kg),空白及模型组生理盐水灌胃。观察小鼠10、13、16周龄唾液流率的变化,18周龄时取颌下腺,对颌下腺进行HE染色,免疫组化法测AQP5的表达。结果与模型组相比,麦冬地芍汤组小鼠16周龄时唾液流率明显改善(P<0.01),18周龄时,麦冬地芍汤组组织学评分低于模型组(P<0.05),AQP5的表达较模型组明显上调(P<0.05)。结论麦冬地芍汤对NOD小鼠颌下腺有保护作用,其机制可能与其上调AQP5的表达相关。展开更多
文摘Nonalcoholic fatty liver disease(NAFLD) is well described as a common cause of chronic liver disease, mostly in the obese population. It refers to a spectrum of chronic liver disease that starts with simple steatosis than progresses to nonalcoholic steatohepatitis and cirrhosis in patients without significant alcohol consumption. NAFLD in the non-obese population has been increasingly reported and studied recently. The pathogenesis of nonobese NAFLD is poorly understood and is related to genetic predisposition, most notably patatin-like phospholipase domain-containing 33 G allele polymorphism that leads to intrahepatic triglyceride accumulation and insulin resistance. Nonobese NAFLD is associated with components of metabolic syndrome and, especially, visceral obesity which seems to be an important etiological factor in this group. Dietary factors and, specifically, a high fructose diet seem to play a role. Cardiovascular events remain the main cause of mortality and morbidity in NAFLD, including in the nonobese population. There is not enough data regarding treatment in non-obese NAFLD patients, but similar to NAFLD in obese subjects, lifestyle changes that include dietary modification, physical activity, and weight loss remain the mainstay of treatment.
基金Science and Technology Project Task Book of Beijing,No.Z171100001717008.
文摘BACKGROUND Nonalcoholic fatty liver disease(NAFLD)has become one of the most common chronic liver diseases in the world.In our early clinical data and questionnaire analysis of NAFLD,it was found that the body mass index of some patients did not meet the diagnostic criteria for overweight or obesity.The consumption of high-temperature-processed foods such as fried food,hot pot and barbecue is closely related to the occurrence of nonobese NAFLD.Reducing the intake of this kind of food can reduce disease severity and improve prognosis.AIM To explore the untargeted metabolomics characteristics of nonobese nonalcoholic fatty liver disease in Sprague-Dawley rats induced by high-temperatureprocessed feed.METHODS Fifty-four male Sprague-Dawley rats were divided into three groups:The control group received a standard diet;the nonfried soybeans(NDFS)group received 60%NDFS and 40%basic feed and the dry-fried soybeans(DFS)group received 60%DFS and 40%basic feed.Six rats were sacrificed at week 4,8,and 12 in each group.The food intake,body weight,Lee’s index,liver index,serological index and hepatic histopathology were assessed.Untargeted metabolomics characteristics were used to analyze the changes in liver metabolites of rats at week 12.Correlations between metabolites and pathology scores between the DFS and control groups and between the DFS and NDFS groups were analyzed.We selected some of the metabolites,both within the pathway and outside of the pathway,to explain preliminarily the difference in liver pathology in the three groups of rats.RESULTS There were no statistically significant differences in the food intake,body weight,Lee's index or serological index between the DFS group and the control group(P>0.05).At week 8 and week 12,the steatosis scores in the DFS group were significantly higher than those in the other two groups(P<0.05).At week 12,the liver index of the DFS group was the lowest(NDFS group vs DFS group,P<0.05).The fibrosis score in the DFS group was significantly higher than those in the other two g
文摘Nonalcoholic fatty liver disease(NAFLD)is the most common liver disease worldwide,with an estimated prevalence of 25% globally.NAFLD is closely associated with metabolic syndrome,which are both becoming increasingly more common with increasing rates of insulin resistance,dyslipidemia,and hypertension.Although NAFLD is strongly associated with obesity,lean or nonobese NAFLD is a relatively new phenotype and occurs in patients without increased waist circumference and with or without visceral fat.Currently,there is limited literature comparing and illustrating the differences between lean/nonobese and obese NAFLD patients with regard to risk factors,pathophysiology,and clinical outcomes.In this review,we aim to define and further delineate different phenotypes of NAFLD and present a comprehensive review on the prevalence,incidence,risk factors,genetic predisposition,and pathophysiology.Furthermore,we discuss and compare the clinical outcomes,such as insulin resistance,dyslipidemia,hypertension,coronary artery disease,mortality,and progression to nonalcoholic steatohepatitis,among lean/nonobese and obese NAFLD patients.Finally,we summarize the most up to date current management of NAFLD,including lifestyle interventions,pharmacologic therapies,and surgical options.
文摘Background Sjǒgren syndrome （SS） is a systematic autoimmune disease, on which traditional therapeutic agents show limited effect. More effective agents with longer-lasting and fewer side effects are needed in the clinic. The aim of this study was to investigate the effects of Ganoderma lucindum spores （GLS） on sialoadenitis of nonobese diabetic （NOD） mice. Methods Thirty-two female NOD mice were assigned randomly into 4 groups： low-dose GLS-treated （L-GLS） group and high-dose GLS-treated （H-GLS） group, a dexamethasone group, and a normal saline （NS） control group. Stimulated total saliva flow rate （STFR）, area of lymphocytic infiltration in submandibular glands and ratios of CD4^＋ and CD8^＋ T lymphocytes and B lymphocytes in peripheral blood as well as apoptosis of these subsets and serum IgG level were tested after 10 weeks of treatments. Differences among the groups were analyzed by one-way analysis of variance （ANOVA）, Student-Newman-Keuls Test （SNK） was used between each two groups and a P 〈0.05 was considered statistically significant. Results STFR of the high-dose GLS group increased significantly after a 10-week treatment compared with those of the NS control group （P 〈0.05）. The incidence of sialoadenitis in GLS-treated NOD mice groups showed no significant difference compared with the control group （P 〉0.05）, but the area of lymphocytic foci in both the H-GLS and L-GLS groups decreased significantly to 50% of the NS control group （P 〈0.05）; the ratio of CD4^＋/CD8^＋ T lymphocytes and apoptosis of B lymphocytes of NOD mice with sialoadenitis were less and apoptosis of CD4^＋ and CD8^＋ T lymphocytes were significantly increased compared with the control group （P 〈0.05）. After pretreatment with H-GLS before sialoadenitis onset, the ratio of CD4^＋/CD8^＋ T lymphocyte and the serum IgG levels of NOD mice decreased significantly （P 〈0.05）. Conclusions Pretreatment with H-GLS can relieve symptoms of sialoadenitis in NOD mice. GL
文摘Background Sjogren syndrome （SS） is an autoimmune disorder characterized by chronic lymphocytic infiltration and decreased secretion in salivary glands. Apoptosis is one of the possible mechanisms involved in acinar epithelial destruction in SS. The role of apoptosis in the initiation and effect phase of sialoadenitis is still controversial. The aim of this study was to observe the roles of apoptosis-associated proteins and serum IgG levels in sialoadenitis progression in nonobese diabetic （NOD） mice. Methods 2-, 5-, 10-, 15-, 20-week female NOD and matched BALB/c control mice were selected. Saliva and tear flow rate were measured. Serum IgG level was tested by enzyme-linked immunosorbent assay （ELISA）. Number of lymphocyte foci （NLF） in submandibular glands （SMGs） was counted under routine hematoxylin/eosin-stained sections. Expression of Fas, Bcl-2 and procaspase3 proteins as well as apoptotic cells in the SMGs were detected by immunohistochemical staining and by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling （TUNEL） assay respectively. Results Decreased stimulated total flow rate （STFR） and lymphocyte foci in SMGs were first observed in the 10-week NOD group. STFR was negatively correlated with NLF （P〈0.05）. Serum IgG in NOD mice was significantly higher than that of the control group （P〈0.05） and showed a positive correlation with NLF （P〈0.05）. Fas expression in SMGs acinar cells in NOD mice increased with age and was significantly higher compared with that in the control group. Bcl-2 expression and procaspase3 expression in SMG acinar cells in each NOD group were lower compared with those of the age-matched control mice. Conclusion Abnormal expression of Fas and Bcl-2 in the SMGs and higher level of serum IgG may contribute to the initiation of sialoadenitis and cause the glandular destruction in NOD mice.
基金Supported by the UNKP-20-4-I New National Excellence Program of the Ministry for Innovation and Technology from the source of the National Research,Development and Innovation Fund.
文摘The granin glycoprotein family consists of nine acidic proteins;chromogranin A(CgA),chromogranin B(CgB),and secretogranin II–VIII.They are produced by a wide range of neuronal,neuroendocrine,and endocrine cells throughout the human body.Their major intracellular function is to sort peptides and proteins into secretory granules,but their cleavage products also take part in the extracellular regulation of diverse biological processes.The contribution of granins to carbohydrate metabolism and diabetes mellitus is a recent research area.CgA is associated with glucose homeostasis and the progression of type 1 diabetes.WE-14,CgA10-19,and CgA43-52 are peptide derivates of CgA,and act as CD4+or CD8+autoantigens in type 1 diabetes,whereas pancreastatin(PST)and catestatin have regulatory effects in carbohydrate metabolism.Furthermore,PST is related to gestational and type 2 diabetes.CgB has a crucial role in physiological insulin secretion.Secretogranins II and III have angiogenic activity in diabetic retinopathy(DR),and are novel targets in recent DR studies.Ongoing studies are beginning to investigate the potential use of granin derivatives as drugs to treat diabetes based on the divergent relationships between granins and different types of diabetes.
基金supported by the Key Specialty Construction Project of Pudong Health and Family Planning Commission of Shanghai [Grant No.PWZzk2017-29]Outstanding Leaders Training Program of Pudong Health Bureau of Shanghai [Grant No.PWRI2018-02]National Natural Science Foundation of China [81170738,81671595]
文摘Prehypertension and hypertension have been considered significant public health issues worldwide[1].Disproportionate body fat distribution(excess fat stored in the liver and visceral compartment)is a well-established risk factor for prehypertension/hypertension[2].However,the most commonly used anthropometric indicators,such as body mass index(BMI)and waist circumference(WC)。
基金The Famous Doctor Inherits Workstation of“3+3 Inheritance Program”of Beijing Administration of Traditional Chinese Medicine
文摘Objective:The objective of this study was to observe the effect of Bushen Qingre Yuyin Decoction on the spleen index,submandibular gland index,salivary secretion,submandibular gland morphology,and aquaporin 5(AQP5)expression levels in the submandibular gland.Materials and Methods:Fifty 8-week-old nonobese diabetic(NOD)female mice were randomly divided into the following five groups:model;hydroxychloroquine;and high-,medium-,and low-dose Bushen Qingre Yuyin Decoction prescription.The blank group consisted of ten 8-week-old Balb/C rats.Bushen Yuyin Qingre Decoction was administered by gavage at 20,40,and 80 g/kg in the low-,medium-,and high-dose groups,respectively.The hydroxychloroquine group was administered hydroxychloroquine at a dose of 80 mg/kg by gavage.The normal and model groups were administered the same amount of saline.After the different administrations,the amount of salivary secretion in the mice was regularly observed.After 12 weeks,the mice were sacrificed,and their submandibular gland tissues were excised,stained with hematoxylin-eosin,and pathologically scored.AQP5 expression levels in the tissues were detected using real-time polymerase chain reaction.Results:The spleen index of mice in the high-dose group was higher than that in the model group(P<0.05),and the submandibular gland index of the hydroxychloroquine group and all the Bushen Qingre Yuyin Decoction prescription groups was higher than that in the model group(P<0.05).After 6 weeks,the salivary volume of the hydroxychloroquine group and all the Bushen Qingre Yuyin Decoction prescription groups was significantly higher than that of the model group(P<0.05);the amount of lymphocyte infiltration in mice among each administration group was significantly reduced(P<0.05);and the AQP5 expression levels in the submandibular glands of mice had significantly increased in the hydroxychloroquine and high-dose groups(P<0.05),with the high-dose group showing the best effect.Conclusion:Bushen Qingre Yuyin Decoction can effectively increase the spleen and
文摘目的观察麦冬地芍汤对干燥综合征模型(nonobese diabetic mouse,NOD)小鼠颌下腺的保护作用。方法取雌性NOD小鼠33只,随机分为模型组、羟氯喹组、麦冬地芍汤组,另取ICR(Institute of Cancer Research)小鼠8只作为空白组。适应性喂养一周后,羟氯喹组、麦冬地芍汤组分别灌服羟氯喹溶液(0.1 g/kg,20 ml/kg)及麦冬地芍汤(10 g/kg,20 ml/kg),空白及模型组生理盐水灌胃。观察小鼠10、13、16周龄唾液流率的变化,18周龄时取颌下腺,对颌下腺进行HE染色,免疫组化法测AQP5的表达。结果与模型组相比,麦冬地芍汤组小鼠16周龄时唾液流率明显改善(P<0.01),18周龄时,麦冬地芍汤组组织学评分低于模型组(P<0.05),AQP5的表达较模型组明显上调(P<0.05)。结论麦冬地芍汤对NOD小鼠颌下腺有保护作用,其机制可能与其上调AQP5的表达相关。
