AIM To elucidate the role of hepatitis G virus (HGV) infection in chronic non A E hepatitis and sequence the partial NS5 genome of HGV in the serum of a Chinese patient with chronic non A E hepatitis. METHODS T...AIM To elucidate the role of hepatitis G virus (HGV) infection in chronic non A E hepatitis and sequence the partial NS5 genome of HGV in the serum of a Chinese patient with chronic non A E hepatitis. METHODS Total nucleic acids were extracted from the sera of patients with chronic non A E hepatitis and subjected to reverse transcriptase nested polymerase chain reaction (RT nested PCR) using primers from the putative NS5 region of HGV genome. 994bp cDNA was obtained from the positive serum and was directly sequenced using dideoxy mediated chain termination method after purified with electrophoresis of polyacrylamide gels. RESULTS HGV RNA was detected in 1 of 35 patients with chronic non A E hepatitis. Compared with the 2 American HGV isolates (PNF2161 and R10291), the homology of HGV NS5 gene of this Beijing isolate (HG G) was 88 0% and 89 2% respectively, while compared with West African isolate (GBV C), it was 93 5%. This patient had persistent increase of ALT but soon became normal after interferon therapy with persistent positive HGV RNA. CONCLUSION HGV is one of the causes of chronic non A E hepatitis, however, it may not be a very important cause. The nucleotide sequence of partial NS5 gene of HG G is highly homologous with the West Africa isolate.展开更多
According to the published nucleotide sequence of SEN virus genome,specific primers were designed and synthesizedFrom the serum of a Chinese patient with non-A-E hepatitis,two long fragments(totally 3175bp) spanning...According to the published nucleotide sequence of SEN virus genome,specific primers were designed and synthesizedFrom the serum of a Chinese patient with non-A-E hepatitis,two long fragments(totally 3175bp) spanning the complete coding region of SENV-D variant gene were amplified by semi-nested PCRThe amplified fragments were cloned and sequencedThe nucleotide sequence homology of this Chinese strain with SENV-D(AX025730),SENV-D(AB059352) and TTV(AB028668) were 90%,88% and 91% respectivelyThe protein encoded by ORF1 has two putative conserved sequence motifs relating to the replication of the virus and,besides,a conserved ATP/GTP-binding motif and several highly conserved protein kinase phosphorylation展开更多
文摘AIM To elucidate the role of hepatitis G virus (HGV) infection in chronic non A E hepatitis and sequence the partial NS5 genome of HGV in the serum of a Chinese patient with chronic non A E hepatitis. METHODS Total nucleic acids were extracted from the sera of patients with chronic non A E hepatitis and subjected to reverse transcriptase nested polymerase chain reaction (RT nested PCR) using primers from the putative NS5 region of HGV genome. 994bp cDNA was obtained from the positive serum and was directly sequenced using dideoxy mediated chain termination method after purified with electrophoresis of polyacrylamide gels. RESULTS HGV RNA was detected in 1 of 35 patients with chronic non A E hepatitis. Compared with the 2 American HGV isolates (PNF2161 and R10291), the homology of HGV NS5 gene of this Beijing isolate (HG G) was 88 0% and 89 2% respectively, while compared with West African isolate (GBV C), it was 93 5%. This patient had persistent increase of ALT but soon became normal after interferon therapy with persistent positive HGV RNA. CONCLUSION HGV is one of the causes of chronic non A E hepatitis, however, it may not be a very important cause. The nucleotide sequence of partial NS5 gene of HG G is highly homologous with the West Africa isolate.
文摘According to the published nucleotide sequence of SEN virus genome,specific primers were designed and synthesizedFrom the serum of a Chinese patient with non-A-E hepatitis,two long fragments(totally 3175bp) spanning the complete coding region of SENV-D variant gene were amplified by semi-nested PCRThe amplified fragments were cloned and sequencedThe nucleotide sequence homology of this Chinese strain with SENV-D(AX025730),SENV-D(AB059352) and TTV(AB028668) were 90%,88% and 91% respectivelyThe protein encoded by ORF1 has two putative conserved sequence motifs relating to the replication of the virus and,besides,a conserved ATP/GTP-binding motif and several highly conserved protein kinase phosphorylation
