[ Objective] Effects of sub-chronic intoxication of 1,8-cineole on body weights, routine blood indexes and biochemical indexes of mice were investigated. [Method] One hundred and sixty mice with body weights of 15 -17...[ Objective] Effects of sub-chronic intoxication of 1,8-cineole on body weights, routine blood indexes and biochemical indexes of mice were investigated. [Method] One hundred and sixty mice with body weights of 15 -17 g were randomly divided into four groups （forty mice per group）. Mice were injected to 1, 8 - cineole with doses of 192.45,64. 15 and 21.38 mg/kg body weight （ test groups） and the water solution of tween-80 with a volume fraction of 0.5% （ control group） respectively. Each mouse was administered orally at the dose of 0.2 mL per 10 g body weight once a day consecutively for 90 d. The body weight, routine blood indexes and serum biochemical indexes of mice were determined on the 30^th d, 60^th d, 90^th d and the 30^th d after stopping the administration of 1,8-cineole. [ Result] The effects of 1, 8-cineole on the body weight, routine blood indexes and serum biochemical indexes of mice with the doses of 64.15 and 21.38 mg/kg body weight had no statistically significant difference compared with the control group （P 〉0.05 ）. 1, 8-cineole with the dose of 192.45 mg/kg body weight exhibited different influences on routine blood indexes and serum biochemical indexes of mice after the oral administration of 1,8-cineole for 60 d and 90 d, and statistically significant differences in many blood biochemical indexes were observed （P 〈 0.05 ）. However, the differences in routine blood indexes and serum biochemical indexes were not statistically significant between the test groups and the control group at the 30＇h d after stopping the administration of 1, 8-cineole （ P 〉 0.05）. [Condusion] 1,8-cineole had sub-chronic oral toxicity to mice. The no observed adverse effect level （NOAEL） of 1,8-cineolc was 64.15 mg/kg body weight and the lowest observed adverse effect level （LOAEL） of 1,8-cineole was 192.45 mg/kg body weight. Effects of 1, 8-cineole on blood biochemical indexes of mice were in short term and reversible.展开更多
目的在研究嘧草硫醚原药对大鼠的亚慢性经口毒性基础上,初步探讨其作用的靶器官。方法将80只SPF级SD大鼠随机分成4组,雌雄各半,分别用含0、15、60和240 mg/kg嘧草硫醚原药的饲料连续90 d经口喂饲,观察各组动物实验中的临床表现、食物利...目的在研究嘧草硫醚原药对大鼠的亚慢性经口毒性基础上,初步探讨其作用的靶器官。方法将80只SPF级SD大鼠随机分成4组,雌雄各半,分别用含0、15、60和240 mg/kg嘧草硫醚原药的饲料连续90 d经口喂饲,观察各组动物实验中的临床表现、食物利用率、体重变化情况,实验末期测定相关的血液和尿液学指标、脏器重量及脏器系数、病理组织学变化。结果试验期间,雌雄各剂量组动物活动正常,未见明显中毒体征和异常表现。与相应的对照组相比,中、高剂量组大鼠淋巴细胞百分比(The Ratio of Lymphocyte,LYM%)明显降低,中、高剂量组大鼠的中性粒细胞绝对值百分比(The Absolute Ratio of Neutrophils,GRA%)明显升高。中、高剂量组雄鼠尿素氮(Urinary Nitrogen,BUN)显著升高;中、高剂量组大鼠肌酐(Creatinine,CREA)升高;中、高剂量组雌鼠的白球比(The Albumin and Globulin Ratio,A/G)明显增高;中、高剂量组雌鼠总蛋白(Total Protein,TP)和球蛋白(Globulin,GLB)明显降低。高剂量雌鼠的肝脏重量及肝脏系数明显升高。病理结果显示高剂量组部分动物肾间质有少量炎细胞浸润和肝脏有轻度脂变。结论嘧草硫醚原药对雌、雄SD大鼠亚慢性经口毒性试验的最大无作用剂量(No Observed Adverse Effect Level,NOAEL)分别为11. 8 mg/(kg·bw·d)和11. 2 mg/(kg·bw·d),其长期暴露对动物肝肾具有潜在的毒性作用。展开更多
基金Supported by the Incubation Program for Science and Technology Innovative Research Team in Sichuan Province of China(2011-JTD0035)Program of Department of Education in Sichuan Province of China(10ZB050)the Program of Department of Education in Sichuan Province of China(11ZZ022)
文摘[ Objective] Effects of sub-chronic intoxication of 1,8-cineole on body weights, routine blood indexes and biochemical indexes of mice were investigated. [Method] One hundred and sixty mice with body weights of 15 -17 g were randomly divided into four groups （forty mice per group）. Mice were injected to 1, 8 - cineole with doses of 192.45,64. 15 and 21.38 mg/kg body weight （ test groups） and the water solution of tween-80 with a volume fraction of 0.5% （ control group） respectively. Each mouse was administered orally at the dose of 0.2 mL per 10 g body weight once a day consecutively for 90 d. The body weight, routine blood indexes and serum biochemical indexes of mice were determined on the 30^th d, 60^th d, 90^th d and the 30^th d after stopping the administration of 1,8-cineole. [ Result] The effects of 1, 8-cineole on the body weight, routine blood indexes and serum biochemical indexes of mice with the doses of 64.15 and 21.38 mg/kg body weight had no statistically significant difference compared with the control group （P 〉0.05 ）. 1, 8-cineole with the dose of 192.45 mg/kg body weight exhibited different influences on routine blood indexes and serum biochemical indexes of mice after the oral administration of 1,8-cineole for 60 d and 90 d, and statistically significant differences in many blood biochemical indexes were observed （P 〈 0.05 ）. However, the differences in routine blood indexes and serum biochemical indexes were not statistically significant between the test groups and the control group at the 30＇h d after stopping the administration of 1, 8-cineole （ P 〉 0.05）. [Condusion] 1,8-cineole had sub-chronic oral toxicity to mice. The no observed adverse effect level （NOAEL） of 1,8-cineolc was 64.15 mg/kg body weight and the lowest observed adverse effect level （LOAEL） of 1,8-cineole was 192.45 mg/kg body weight. Effects of 1, 8-cineole on blood biochemical indexes of mice were in short term and reversible.
文摘目的在研究嘧草硫醚原药对大鼠的亚慢性经口毒性基础上,初步探讨其作用的靶器官。方法将80只SPF级SD大鼠随机分成4组,雌雄各半,分别用含0、15、60和240 mg/kg嘧草硫醚原药的饲料连续90 d经口喂饲,观察各组动物实验中的临床表现、食物利用率、体重变化情况,实验末期测定相关的血液和尿液学指标、脏器重量及脏器系数、病理组织学变化。结果试验期间,雌雄各剂量组动物活动正常,未见明显中毒体征和异常表现。与相应的对照组相比,中、高剂量组大鼠淋巴细胞百分比(The Ratio of Lymphocyte,LYM%)明显降低,中、高剂量组大鼠的中性粒细胞绝对值百分比(The Absolute Ratio of Neutrophils,GRA%)明显升高。中、高剂量组雄鼠尿素氮(Urinary Nitrogen,BUN)显著升高;中、高剂量组大鼠肌酐(Creatinine,CREA)升高;中、高剂量组雌鼠的白球比(The Albumin and Globulin Ratio,A/G)明显增高;中、高剂量组雌鼠总蛋白(Total Protein,TP)和球蛋白(Globulin,GLB)明显降低。高剂量雌鼠的肝脏重量及肝脏系数明显升高。病理结果显示高剂量组部分动物肾间质有少量炎细胞浸润和肝脏有轻度脂变。结论嘧草硫醚原药对雌、雄SD大鼠亚慢性经口毒性试验的最大无作用剂量(No Observed Adverse Effect Level,NOAEL)分别为11. 8 mg/(kg·bw·d)和11. 2 mg/(kg·bw·d),其长期暴露对动物肝肾具有潜在的毒性作用。
