A time-of-arrival(TOA) system based on GPS technology for locating VHF radiation sources from lightning has been developed and used in observation sites in the northern Shandong Province,China.The 3D images of the lig...A time-of-arrival(TOA) system based on GPS technology for locating VHF radiation sources from lightning has been developed and used in observation sites in the northern Shandong Province,China.The 3D images of the lightning progression have been obtained successfully for the first time in China.The 3D-channel evolutions of typical negative CG,positive CG and IC lightning flashes have been discussed together with the data of fast electric field change.It was found that significant differences existed between the negative and positive CG lightning flashes in terms of the initiation and propagation of the radiation sources.The preliminary breakdown of a negative CG lightning flash propagated at a speed about 5.2×104 m/s.The stepped leader of negative CG lightning flashes was trigged by negative initial breakdown.Thereafter,it propagated downward at a speed of 1.3×105 m/s.The initial process of the positive CG lightning flashes was also a propagation process of negative streamer.These streamers propagated dominantly horizontally in the positive charge region and accumulated positive charges at the origin of the lightning,and as a consequence,initiated downward positive streamers.A new type of lightning discharge that was triggered by a narrow bipolar pulse(NBP) is discussed in this study.The NBP was originated at altitude of about 10.5 km in the upper positive charge region.As a distinct difference from normal IC flash,its channels extended horizontally all around and produced a lot of radiation sources.The source power of the NBP could approach 16.7 kW,which is much greater than that of normal lightning discharge ranging between 100 mW and 500 W.The 3D propagation of this new type of lightning discharge was observed and obtained for the first time in China.The possible initiation mechanism of this new type of light-ning is discussed here.展开更多
AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS: The apoptosis of duck embryo fibroblasts (DEF) i...AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS: The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI). RESULTS: By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/ PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non- apoptotic cells decreased.展开更多
基金supported by National Natural Science Foundation of China (Grant No.40775004)Knowledge Innovation Program of the Chinese Academy of Sciences (Grant No.KZCX2-YW-206-2)the R & D Special Fund for Public Welfare Industry (meteorology) (Grant No.GYHY2007622)
文摘A time-of-arrival(TOA) system based on GPS technology for locating VHF radiation sources from lightning has been developed and used in observation sites in the northern Shandong Province,China.The 3D images of the lightning progression have been obtained successfully for the first time in China.The 3D-channel evolutions of typical negative CG,positive CG and IC lightning flashes have been discussed together with the data of fast electric field change.It was found that significant differences existed between the negative and positive CG lightning flashes in terms of the initiation and propagation of the radiation sources.The preliminary breakdown of a negative CG lightning flash propagated at a speed about 5.2×104 m/s.The stepped leader of negative CG lightning flashes was trigged by negative initial breakdown.Thereafter,it propagated downward at a speed of 1.3×105 m/s.The initial process of the positive CG lightning flashes was also a propagation process of negative streamer.These streamers propagated dominantly horizontally in the positive charge region and accumulated positive charges at the origin of the lightning,and as a consequence,initiated downward positive streamers.A new type of lightning discharge that was triggered by a narrow bipolar pulse(NBP) is discussed in this study.The NBP was originated at altitude of about 10.5 km in the upper positive charge region.As a distinct difference from normal IC flash,its channels extended horizontally all around and produced a lot of radiation sources.The source power of the NBP could approach 16.7 kW,which is much greater than that of normal lightning discharge ranging between 100 mW and 500 W.The 3D propagation of this new type of lightning discharge was observed and obtained for the first time in China.The possible initiation mechanism of this new type of light-ning is discussed here.
基金The National Natural Science Foundation of China, No. 39970561The Key Projects in the National Science and Technology Pillar Program, 2007Z06-017+2 种基金Program for New Century Excellent Talents from Universities, Chinese Ministry of Education, No. NCET-04-0906/NCET-06-0818Fund of the Discipline Leaders of Sichuan Province, No. SZD0418Culture Fund for Excellent Doctoral Dissertations of Sichuan Agricultural University, 2008scybpy-1
文摘AIM: To achieve a better understanding of the pathogenesis of new type gosling viral enteritis virus (NGVEV) and the relationship between NGVEV and host cells. METHODS: The apoptosis of duck embryo fibroblasts (DEF) induced by NGVEV was investigated by fluorescence-activated cell sorter (FACS) and fluorescence microscope after the cells were stained with Annexin V-FITC and propidium iodide (PI). RESULTS: By staining cells with a combination of fluorescein annexin V-FITC and PI, it is possible to distinguish and quantitatively analyze non-apoptotic cells (Annexin V-FITC negative/PI negative), early apoptotic cells (Annexin V-FITC positive/PI negative), late apoptotic/necrotic cells (Annexin V-FITC positive/ PI positive) and dead cells (Annexin V-FITC negative/PI positive) through flow cytometry and fluorescence microscope. The percentage of apoptotic cells increased with the incubation time and reached a maximum at 120 h after infection, while the percentage of non- apoptotic cells decreased.
