Fowl adenovirus serotype 4(FAdV-4)strain SD1511 was isolated from chickens with severe inclusion body hepatitis and hydropericardium syndrome in Shandong Province,China.The isolate was cultured in primary chicken embr...Fowl adenovirus serotype 4(FAdV-4)strain SD1511 was isolated from chickens with severe inclusion body hepatitis and hydropericardium syndrome in Shandong Province,China.The isolate was cultured in primary chicken embryo kidney cells.A study of pathogenicity indicated that SD1511 readily infected 7–35-d-old chickens by intramuscular injection and intranasal and oral routes,causing 50%–100%mortality.The 35-d-old chickens suffered more severe infection than 7-and 21-d-old chickens with mortality highest in the intramuscular injection group.The serum from surviving chickens showed potent viral neutralizing capability.The complete genome of SD1511 was sequenced and analyzed.The strain was found to belong to the FAdV-4 cluster with more than 99%identity with the virulent FAdV-4 strains isolated in China in recent years except for some distinct variations,including deletions of open reading frame 27(ORF27),ORF48,and part of ORF19.Our findings suggest that SD1511 might be used as a prototype strain for the study of pathogenesis and vaccine development.展开更多
Objective:To validate traditional claims of usefulness of the Indian plants in management of poisonous snakebite and evaluate the antivenom properties displayed bv the alcoholic extracts of Andrographis paniculata（A....Objective:To validate traditional claims of usefulness of the Indian plants in management of poisonous snakebite and evaluate the antivenom properties displayed bv the alcoholic extracts of Andrographis paniculata（A.paniculata）,Crutera magna（C.magna）.Gloriosa superba （G.superba） and Hydrocotyle javanica（H.javanica）.Methods:These plants were collected, identified and the extracts were prepared bv using conventional Soxhlet ethanol extraction technique.The venom neutralization activity was accessed in mice（20-25g） and number of mortalities was observed against clinically important snake（Naja nigricollis） venom.Present study also deals with in vitro membrane stabilizing activity of these plants against hyposaline induced human red blood corpuscles（HRBC）.Results:Extracts of H.javanica and G.superba gave 80%and 90%protection to mice treated with minimum lethal dose of venom(LD<sub>99</sub>).These two plants showed significant neutralization effect against the venoms of Naja nigricollis venom.H. javanica and G.superba（25-100 mg/ml.） produced significant changes of membrane stabilization of human red blood cells（HRBC） exposed to hyposaline-induced haemolysis.Conclusions:We conclude that probably due to presence of various phytochemicals plays an important role in the anti-venom potential of these Indian medicinal plants against Naja nigricollis venom.The above observations confirmed that.4.paniculata,G,magna.G.superba and H.javanica plant extracts possess potent snake venom neutralizing capacity and could potentially be used as an adjuvants for antivenin therapy in case of snakebite envenomation.especially against the local effects of cobra venoms.展开更多
Antibodies are ideal for controlling the influenza A virus,but their effect on newly emerging strains is unclear.Here,we assessed the neutralization activity of the humanized monoclonal antibodies(mAbs)F10,H98 and H40...Antibodies are ideal for controlling the influenza A virus,but their effect on newly emerging strains is unclear.Here,we assessed the neutralization activity of the humanized monoclonal antibodies(mAbs)F10,H98 and H40 against circulating influenza viruses(H5N1,H1N1,H3N2 and H7N7 and new subtypes viruses H5N6 and H7N9).The results showed that all the three humanized mAbs(F10,H98 and H40)displayed different degrees of virus neutralization activities when encountered with different subtypes of influenza viruses.Remarkably,the humanized monoclonal antibody F10 produced higher and broader neutralization titers(range 25–1.56μg/ml)than those of the other two humanized mAbs(H98(range 50–3.12μg/ml),H40(range 50–5.56μg/ml))to against the viruses H5N1,H1N1,H3N2,H7N7,H5N6 and H7N9.This mAb may represent a new class of heterosubtypic neutralizing humanized mAb that could replace vaccines and chemical drugs.展开更多
Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV...Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV-1 infected peoples is still undetermined. In this paper, we compared the protective function of the neutralizing antibody response in the plasma from LTNP and TP against clade B and clade C pseudoviruses. No difference in the neutralizing activities between the plasma from LTNP and TP was found, which was consistent with the most recent reports. In addition, no correlations between the titer or breadth and CD4+ or viral load in HIV-1 infected individuals were found. The protective roles played by neutralizing antibodies in controlling disease progression of HIV-1 infected people need to be considered in a new viewpoint.展开更多
基金the National Key Technology Research and Development Program of China(No.2015BAD12B01)the China Agriculture Research System(No.CARS-40-K13)
文摘Fowl adenovirus serotype 4(FAdV-4)strain SD1511 was isolated from chickens with severe inclusion body hepatitis and hydropericardium syndrome in Shandong Province,China.The isolate was cultured in primary chicken embryo kidney cells.A study of pathogenicity indicated that SD1511 readily infected 7–35-d-old chickens by intramuscular injection and intranasal and oral routes,causing 50%–100%mortality.The 35-d-old chickens suffered more severe infection than 7-and 21-d-old chickens with mortality highest in the intramuscular injection group.The serum from surviving chickens showed potent viral neutralizing capability.The complete genome of SD1511 was sequenced and analyzed.The strain was found to belong to the FAdV-4 cluster with more than 99%identity with the virulent FAdV-4 strains isolated in China in recent years except for some distinct variations,including deletions of open reading frame 27(ORF27),ORF48,and part of ORF19.Our findings suggest that SD1511 might be used as a prototype strain for the study of pathogenesis and vaccine development.
基金Periyar College of Pharmacecutical Sciences Periyar Maniammai University,Thiruchirappalli,Tamilnadu for his constant support and providingg facilities to carry out this work successfullyKing Institute of Preventive Medicine,Guindy,Chennai,is also thanked for the Naja nigricollis venom support
文摘Objective:To validate traditional claims of usefulness of the Indian plants in management of poisonous snakebite and evaluate the antivenom properties displayed bv the alcoholic extracts of Andrographis paniculata（A.paniculata）,Crutera magna（C.magna）.Gloriosa superba （G.superba） and Hydrocotyle javanica（H.javanica）.Methods:These plants were collected, identified and the extracts were prepared bv using conventional Soxhlet ethanol extraction technique.The venom neutralization activity was accessed in mice（20-25g） and number of mortalities was observed against clinically important snake（Naja nigricollis） venom.Present study also deals with in vitro membrane stabilizing activity of these plants against hyposaline induced human red blood corpuscles（HRBC）.Results:Extracts of H.javanica and G.superba gave 80%and 90%protection to mice treated with minimum lethal dose of venom(LD<sub>99</sub>).These two plants showed significant neutralization effect against the venoms of Naja nigricollis venom.H. javanica and G.superba（25-100 mg/ml.） produced significant changes of membrane stabilization of human red blood cells（HRBC） exposed to hyposaline-induced haemolysis.Conclusions:We conclude that probably due to presence of various phytochemicals plays an important role in the anti-venom potential of these Indian medicinal plants against Naja nigricollis venom.The above observations confirmed that.4.paniculata,G,magna.G.superba and H.javanica plant extracts possess potent snake venom neutralizing capacity and could potentially be used as an adjuvants for antivenin therapy in case of snakebite envenomation.especially against the local effects of cobra venoms.
基金This work was supported by the National Key Research and Development Program of China(2017YFD0501705 and 2016YFD0500203).
文摘Antibodies are ideal for controlling the influenza A virus,but their effect on newly emerging strains is unclear.Here,we assessed the neutralization activity of the humanized monoclonal antibodies(mAbs)F10,H98 and H40 against circulating influenza viruses(H5N1,H1N1,H3N2 and H7N7 and new subtypes viruses H5N6 and H7N9).The results showed that all the three humanized mAbs(F10,H98 and H40)displayed different degrees of virus neutralization activities when encountered with different subtypes of influenza viruses.Remarkably,the humanized monoclonal antibody F10 produced higher and broader neutralization titers(range 25–1.56μg/ml)than those of the other two humanized mAbs(H98(range 50–3.12μg/ml),H40(range 50–5.56μg/ml))to against the viruses H5N1,H1N1,H3N2,H7N7,H5N6 and H7N9.This mAb may represent a new class of heterosubtypic neutralizing humanized mAb that could replace vaccines and chemical drugs.
基金supported in part by the National Key S&T Special Projects on Major Infectious Diseases (Grant No. 2008ZX10001-002, 2008ZX10001-012)the National Natural Science Foundation of China (No. 30700706)
文摘Neutralizing antibodies are considered to be an important protective parameter used in HIV-1 vaccine evaluation. However, the exact role that neutralizing antibodies plays in controlling the disease progression of HIV-1 infected peoples is still undetermined. In this paper, we compared the protective function of the neutralizing antibody response in the plasma from LTNP and TP against clade B and clade C pseudoviruses. No difference in the neutralizing activities between the plasma from LTNP and TP was found, which was consistent with the most recent reports. In addition, no correlations between the titer or breadth and CD4+ or viral load in HIV-1 infected individuals were found. The protective roles played by neutralizing antibodies in controlling disease progression of HIV-1 infected people need to be considered in a new viewpoint.
