This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase 2, 9 (MMP 2, MMP 9), tissue inhibitor 1 of matrix metalloproteinase (TIMP 1), c...This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase 2, 9 (MMP 2, MMP 9), tissue inhibitor 1 of matrix metalloproteinase (TIMP 1), cell adhesion molecule 44 variant 6 (CD44v6), HER2/neu and p53 was investigated in 154 patients with head and neck squamous cell carcinoma (SCC) by ABC and ImmunoMax immunohistochemical method. Their clinical relevance and correlation were analysed. The expression of MMP 2, MMP 9, TIMP 1, CD44v6, HER2/neu and p53 was found in cancer cells in 87.01%, 85.71%, 68.18%, 98.05%, 55.19% and 50.65% cases respectively. Linear regression and correlation analysis revealed that there was close positive relationship ( P <0.05) between the expression of MMP 2 and MMP 9, TIMP 1 and CD44v6, HER2/neu and MMP 9, MMP 2 and p53. Up regulation of MMP 2 was accompanied by advanced T stage ( P <0.01) . There was also a trend of MMP 2 expression being related with tumor metastasis. Increased expression of HER2/neu was found in patients with tumor recurrence( P <0.05). The expression of TIMP 1 was higher in laryngeal cancer than that in pharyngeal cancer, and higher in keratinizing and non keratinizing SCC than that in basaloid SCC( P <0.05). These findings suggested that MMP 2 and MMP 9, HER2/neu and MMP 9, MMP 2 and p53 had a coordinate function in aggression of tumor; that MMP 2 had a more important function than MMP 9 in tumor invasion and metastasis; and that HER2/neu might serve as a biomarker for poor prognosis in HNSCC.展开更多
E-cadherin is present in the epithelial cells and its aberrant expression is correlated with different kinds of head and neck squamocellular carcinomas. The purpose of the present study was to identify the expression ...E-cadherin is present in the epithelial cells and its aberrant expression is correlated with different kinds of head and neck squamocellular carcinomas. The purpose of the present study was to identify the expression particularities of analyzed E-cadherin in rapport with the localization and the differentiation of various head and neck squamocellular carcinomas. 18 biopsy fragments obtained by squamocellular carcinoma patients (larynx, pharynx, hard palate, tongue, submandibular, lip, gingival sulcus, nasal pyramid, maxillary, zygomatic) were processed by immunohistochemical staining. Immunoreactions for E-cadherin in the tumoral cells were examined according to the score: 0 (0% positive cells of specimen);1 (30% of specimen). The immunohistochemical staining indicated the presence of 12 cases of well-differentiated squamocellular carcinoma (7 cases with score 3, 3 cases with score 2 and 1 case with score 1). Moderately-differentiated carcinomas were observed in the 3 cases (2 with score 2 and 1 with score 1). The poorly-differentiated histopathological type was present in 3 cases (all with score 1). Three types of E-cadherin distribution patterns were found: cytoplasmatic;cytoplasmatic and membranar;membranar. The presence of maximum score (value 3) of E-cadherin was found in well-differentiated squamocellular carcinomas of laryngeal, tongue, lip, nasal pyramid, and zygomatic area origin. A lower value of the score was present in the less differentiated histopathological type.展开更多
文摘This study aimed to explore the molecular mechanism in tumor invasion and metastasis. The expression of matrix metalloproteinase 2, 9 (MMP 2, MMP 9), tissue inhibitor 1 of matrix metalloproteinase (TIMP 1), cell adhesion molecule 44 variant 6 (CD44v6), HER2/neu and p53 was investigated in 154 patients with head and neck squamous cell carcinoma (SCC) by ABC and ImmunoMax immunohistochemical method. Their clinical relevance and correlation were analysed. The expression of MMP 2, MMP 9, TIMP 1, CD44v6, HER2/neu and p53 was found in cancer cells in 87.01%, 85.71%, 68.18%, 98.05%, 55.19% and 50.65% cases respectively. Linear regression and correlation analysis revealed that there was close positive relationship ( P <0.05) between the expression of MMP 2 and MMP 9, TIMP 1 and CD44v6, HER2/neu and MMP 9, MMP 2 and p53. Up regulation of MMP 2 was accompanied by advanced T stage ( P <0.01) . There was also a trend of MMP 2 expression being related with tumor metastasis. Increased expression of HER2/neu was found in patients with tumor recurrence( P <0.05). The expression of TIMP 1 was higher in laryngeal cancer than that in pharyngeal cancer, and higher in keratinizing and non keratinizing SCC than that in basaloid SCC( P <0.05). These findings suggested that MMP 2 and MMP 9, HER2/neu and MMP 9, MMP 2 and p53 had a coordinate function in aggression of tumor; that MMP 2 had a more important function than MMP 9 in tumor invasion and metastasis; and that HER2/neu might serve as a biomarker for poor prognosis in HNSCC.
文摘E-cadherin is present in the epithelial cells and its aberrant expression is correlated with different kinds of head and neck squamocellular carcinomas. The purpose of the present study was to identify the expression particularities of analyzed E-cadherin in rapport with the localization and the differentiation of various head and neck squamocellular carcinomas. 18 biopsy fragments obtained by squamocellular carcinoma patients (larynx, pharynx, hard palate, tongue, submandibular, lip, gingival sulcus, nasal pyramid, maxillary, zygomatic) were processed by immunohistochemical staining. Immunoreactions for E-cadherin in the tumoral cells were examined according to the score: 0 (0% positive cells of specimen);1 (30% of specimen). The immunohistochemical staining indicated the presence of 12 cases of well-differentiated squamocellular carcinoma (7 cases with score 3, 3 cases with score 2 and 1 case with score 1). Moderately-differentiated carcinomas were observed in the 3 cases (2 with score 2 and 1 with score 1). The poorly-differentiated histopathological type was present in 3 cases (all with score 1). Three types of E-cadherin distribution patterns were found: cytoplasmatic;cytoplasmatic and membranar;membranar. The presence of maximum score (value 3) of E-cadherin was found in well-differentiated squamocellular carcinomas of laryngeal, tongue, lip, nasal pyramid, and zygomatic area origin. A lower value of the score was present in the less differentiated histopathological type.
