: Spraying a 1–2 mmol/L solution of NaHSO3 on the leaves of wild-type rice (Oryza sativa L.) Kitaake (WT), phosphoenolpyruvate carboxylase (PEPC) transgenic (PC) rice and PEPC+phosphate dikinase (PPDK) transgenic ric...: Spraying a 1–2 mmol/L solution of NaHSO3 on the leaves of wild-type rice (Oryza sativa L.) Kitaake (WT), phosphoenolpyruvate carboxylase (PEPC) transgenic (PC) rice and PEPC+phosphate dikinase (PPDK) transgenic rice (PC+PK), in which the germplasm was transformed with wild-type Kitaake as the gene receptor, resulted in an enhancement of the net photosynthetic rate by 23.0%, 28.8%, and 34.4%, respectively, for more than 3 d. It was also observed that NaHSO3 application caused an increase in the ATP content in leaves. Spraying PMS (a cofactor catalysing the photophosphorylation cycle) and NaHSO3 separately or together on leaves resulted in an increase in photosynthesis with all treatments. There was no additional effect on photosynthetic rate when the mixture was applied, suggesting that the mechanism by which NaHSO3 promotes photosynthesis is similar to the mechanism by which PMS acts and that both of compounds enhanced the supply of ATP. After spraying a solution of NaHSO3 on leaves, compared with the WT Kitaake rice, a greater enhancement of net photosynthetic rate was observed in PEPC transgenic (PC) and PEPC+PPDK transgenic (PC+PK) rice, with the greatest increase being observed in the latter group. Therefore ATP supply may become the limiting factor that concentrates CO2 in rice leaves transformed with an exogenous PEPC gene and exogenous PEPC+PPDK genes.展开更多
对2 m mol/L(处理Ⅰ),5 m mol/L(处理Ⅱ)和10 m mol/L(处理Ⅲ)NaHSO3处理后的油桐叶片荧光参数、叶绿素、可溶性糖、可溶性蛋白质含量和叶生物量进行测定,结果表明:NaHSO3处理油桐以后能引起叶绿素a,叶绿素b,类胡萝卜素和总叶绿素含量增...对2 m mol/L(处理Ⅰ),5 m mol/L(处理Ⅱ)和10 m mol/L(处理Ⅲ)NaHSO3处理后的油桐叶片荧光参数、叶绿素、可溶性糖、可溶性蛋白质含量和叶生物量进行测定,结果表明:NaHSO3处理油桐以后能引起叶绿素a,叶绿素b,类胡萝卜素和总叶绿素含量增加.饱和脉冲模式下的表观光合电子传递速率和247μmol/m2.s光下表观光合电子传递速率增加,可溶性糖、可溶性蛋白质含量、单位面积鲜重和干重增加,且这种增加作用至少能持续12天.在处理的3种浓度中以2 m mol/L NaHSO3效果最好.展开更多
基金国家自然科学基金，the National Key Project of the International Cooperation for Science and Technology
文摘: Spraying a 1–2 mmol/L solution of NaHSO3 on the leaves of wild-type rice (Oryza sativa L.) Kitaake (WT), phosphoenolpyruvate carboxylase (PEPC) transgenic (PC) rice and PEPC+phosphate dikinase (PPDK) transgenic rice (PC+PK), in which the germplasm was transformed with wild-type Kitaake as the gene receptor, resulted in an enhancement of the net photosynthetic rate by 23.0%, 28.8%, and 34.4%, respectively, for more than 3 d. It was also observed that NaHSO3 application caused an increase in the ATP content in leaves. Spraying PMS (a cofactor catalysing the photophosphorylation cycle) and NaHSO3 separately or together on leaves resulted in an increase in photosynthesis with all treatments. There was no additional effect on photosynthetic rate when the mixture was applied, suggesting that the mechanism by which NaHSO3 promotes photosynthesis is similar to the mechanism by which PMS acts and that both of compounds enhanced the supply of ATP. After spraying a solution of NaHSO3 on leaves, compared with the WT Kitaake rice, a greater enhancement of net photosynthetic rate was observed in PEPC transgenic (PC) and PEPC+PPDK transgenic (PC+PK) rice, with the greatest increase being observed in the latter group. Therefore ATP supply may become the limiting factor that concentrates CO2 in rice leaves transformed with an exogenous PEPC gene and exogenous PEPC+PPDK genes.
文摘对2 m mol/L(处理Ⅰ),5 m mol/L(处理Ⅱ)和10 m mol/L(处理Ⅲ)NaHSO3处理后的油桐叶片荧光参数、叶绿素、可溶性糖、可溶性蛋白质含量和叶生物量进行测定,结果表明:NaHSO3处理油桐以后能引起叶绿素a,叶绿素b,类胡萝卜素和总叶绿素含量增加.饱和脉冲模式下的表观光合电子传递速率和247μmol/m2.s光下表观光合电子传递速率增加,可溶性糖、可溶性蛋白质含量、单位面积鲜重和干重增加,且这种增加作用至少能持续12天.在处理的3种浓度中以2 m mol/L NaHSO3效果最好.
