目的研究普伐他汀配伍参麦注射液对家兔心肌缺血再灌注损伤的保护作用。方法32只日本大耳白兔随机分为假手术组(Sham组)、心肌缺血再灌注未干预组(IR组)、普伐他汀组(P组)、普伐他汀配伍参麦注射液干预组(P+S组),建立心肌缺血再灌注的...目的研究普伐他汀配伍参麦注射液对家兔心肌缺血再灌注损伤的保护作用。方法32只日本大耳白兔随机分为假手术组(Sham组)、心肌缺血再灌注未干预组(IR组)、普伐他汀组(P组)、普伐他汀配伍参麦注射液干预组(P+S组),建立心肌缺血再灌注的模型。实验中持续监测ECGⅡ导联的变化,实验结束后处死动物,用Evans B lue染色法测量心肌梗死面积,取左心室缺血心肌制成组织匀浆,用试剂盒分别检测一氧化氮合酶(NOS)活性,超氧化物歧化酶(SOD)及丙二醛(MDA)的含量。结果IR组、R组和P+S组均造成明显的心电图动态改变。与IR组比较,P+S组心肌梗死面积、MDA含量均显著减小(P<0.01)。P+S组分别与IR组和P组比较,SOD活性增加,MDA含量下降。P组及P+S组总一氧化氮合酶(tNOS)活性均显著高于IR组(P<0.05),IR组诱导型一氧化氮合酶(iNOS)活性与tNOS活性比值显著大于Sham组、P组和P+S组(P<0.05)。结论普伐他汀配伍参麦注射液对心肌缺血再灌注损伤有良好的保护作用,联合用药优于单一用药。展开更多
We have done consecutive studies to investigate the effects of impaired lipid metabolism on the contractile and re-laxation response of cavernous smooth muscles and to elucidate its pathogenesis: 1) incidence of hyper...We have done consecutive studies to investigate the effects of impaired lipid metabolism on the contractile and re-laxation response of cavernous smooth muscles and to elucidate its pathogenesis: 1) incidence of hyperlipidemia in im-potent patients; 2) erection response to intracavemous injection of papaverine in impotent patients with hyperlipidemia;3) relaxation responses of isolated cavemosal smooth muscles to endothelium-independent and endothelium-dependentvasodilators in impotent patients with hypercholesterolemia or hypertriglyceridemia; 4) involvement of superoxide radi-cal in the impaired endothelium-dependent relaxation of cavemous smooth muscle in hypercholesterolemic rabbits; S)effects of isolated lipoproteins and triglyceride, combined oxidized LDL plus triglyceride, and combined oxidized LDLplus HDL on contractile and relaxation response of rabbit cavemous smooth muscles; 6) involvement of e-NOS in theimpaired endothelium-dependent relaxation of cavemous smooth muscle in hypercholesterolemic rabbit. Hypercholes-terolemia may cause impairment of endothelium-dependent relaxation. Oxidized LDL is the major causative cholesterolof the impaired relaxation response. A chain reaction, the production of superoxide radicals and functional impairmentof eNOS may be a major cause of the functional impairment in the early stages of hypercholesterolemia.展开更多
A series of Benzisolselenazolone (BISA) derivatives were synthesized and evaluated for their antibacterial activities againstE coli. by using LKB-2277 bioactivity monitor. Other bioactivities were tested by the method...A series of Benzisolselenazolone (BISA) derivatives were synthesized and evaluated for their antibacterial activities againstE coli. by using LKB-2277 bioactivity monitor. Other bioactivities were tested by the method of High Throughput Screening for pharmaceutical activity compounds (HTP) BISA derivatives 3b, at the concentration of 40 μg/mL, showed 100%antibacterial activity and 62%inhibition rate of aldose reductase (at the concentration of 5μg/mL). These new compound structures have determined by IR,1H NMR and MS spectra.展开更多
文摘目的研究普伐他汀配伍参麦注射液对家兔心肌缺血再灌注损伤的保护作用。方法32只日本大耳白兔随机分为假手术组(Sham组)、心肌缺血再灌注未干预组(IR组)、普伐他汀组(P组)、普伐他汀配伍参麦注射液干预组(P+S组),建立心肌缺血再灌注的模型。实验中持续监测ECGⅡ导联的变化,实验结束后处死动物,用Evans B lue染色法测量心肌梗死面积,取左心室缺血心肌制成组织匀浆,用试剂盒分别检测一氧化氮合酶(NOS)活性,超氧化物歧化酶(SOD)及丙二醛(MDA)的含量。结果IR组、R组和P+S组均造成明显的心电图动态改变。与IR组比较,P+S组心肌梗死面积、MDA含量均显著减小(P<0.01)。P+S组分别与IR组和P组比较,SOD活性增加,MDA含量下降。P组及P+S组总一氧化氮合酶(tNOS)活性均显著高于IR组(P<0.05),IR组诱导型一氧化氮合酶(iNOS)活性与tNOS活性比值显著大于Sham组、P组和P+S组(P<0.05)。结论普伐他汀配伍参麦注射液对心肌缺血再灌注损伤有良好的保护作用,联合用药优于单一用药。
文摘We have done consecutive studies to investigate the effects of impaired lipid metabolism on the contractile and re-laxation response of cavernous smooth muscles and to elucidate its pathogenesis: 1) incidence of hyperlipidemia in im-potent patients; 2) erection response to intracavemous injection of papaverine in impotent patients with hyperlipidemia;3) relaxation responses of isolated cavemosal smooth muscles to endothelium-independent and endothelium-dependentvasodilators in impotent patients with hypercholesterolemia or hypertriglyceridemia; 4) involvement of superoxide radi-cal in the impaired endothelium-dependent relaxation of cavemous smooth muscle in hypercholesterolemic rabbits; S)effects of isolated lipoproteins and triglyceride, combined oxidized LDL plus triglyceride, and combined oxidized LDLplus HDL on contractile and relaxation response of rabbit cavemous smooth muscles; 6) involvement of e-NOS in theimpaired endothelium-dependent relaxation of cavemous smooth muscle in hypercholesterolemic rabbit. Hypercholes-terolemia may cause impairment of endothelium-dependent relaxation. Oxidized LDL is the major causative cholesterolof the impaired relaxation response. A chain reaction, the production of superoxide radicals and functional impairmentof eNOS may be a major cause of the functional impairment in the early stages of hypercholesterolemia.
基金Supported by Hubei Province Natural Science Fund( 99J0 5 6 )
文摘A series of Benzisolselenazolone (BISA) derivatives were synthesized and evaluated for their antibacterial activities againstE coli. by using LKB-2277 bioactivity monitor. Other bioactivities were tested by the method of High Throughput Screening for pharmaceutical activity compounds (HTP) BISA derivatives 3b, at the concentration of 40 μg/mL, showed 100%antibacterial activity and 62%inhibition rate of aldose reductase (at the concentration of 5μg/mL). These new compound structures have determined by IR,1H NMR and MS spectra.
