目的探讨RhoA及Rho激酶（ROCKI）在子宫腺肌病患者子宫内膜一肌层交界区平滑肌细胞中的表达及其与痛经程度的关系。方法选择2012年3至12月在首都医科大学附属北京妇产医院妇科微创中心因子宫腺肌病行子宫全切除术的32例患者为子宫腺肌...目的探讨RhoA及Rho激酶（ROCKI）在子宫腺肌病患者子宫内膜一肌层交界区平滑肌细胞中的表达及其与痛经程度的关系。方法选择2012年3至12月在首都医科大学附属北京妇产医院妇科微创中心因子宫腺肌病行子宫全切除术的32例患者为子宫腺肌病组（其中增殖期内膜18例，分泌期内膜14例）；同期因宫颈病变或卵巢肿瘤而行子宫切除的患者29例为对照组（其中增殖期内膜12例，分泌期内膜17例）。对两组患者子宫内膜一肌层交界区平滑肌细胞进行分离及原代培养。应用实时荧光定量PCR技术和蛋白印迹法，检测两组及子宫腺肌病组不同程度痛经患者子宫内膜一肌层交界区平滑肌细胞中RhoA和ROCK I mRNA及其蛋白的表达水平。结果（1）子宫腺肌病组子宫内膜一肌层交界区平滑肌细胞中RhoA和ROCKImRNA表达水平无明显周期性变化，增殖期表达水平（分别为1．41±0．16、1．05±0．15）稍高于分泌期（分别为1．17±0．25、0．98±0．10），分别比较，差异均无统计学意义（P〉0．05）；对照组RhoA和ROCKImRNA表达水平存在周期性变化，增殖期表达水平（分别为0．93±0．10、1．00±0．18）高于分泌期（0．48±0．03、0．55±0．05），分别比较，差异均有统计学意义（P〈0．05）；子宫腺肌病组增殖期和分泌期RhoA和ROCKImRNA的表达水平均高于对照组，分别比较，差异均有统计学意义（P〈0．01）。（2）子宫腺肌病组与对照组RhoA及ROCKImRNA、蛋白表达水平均呈正相关关系（r=0．67，P〈0．01；r=0．48，P〈0．01）。（3）RhoA和ROCKImRNA、蛋白表达水平在子宫腺肌病组不同程度痛经患者中依次表现为：重度痛经（1．66±0．19、1．32±0．11）〉中度痛经（1．28±0．12、1．09±0．08）〉轻度痛经（0．93±0．09、0．81±0．06），两两分别比较，差异均有统计学意义（P〈0．05）。结论子宫腺肌病患者子展开更多
目的探讨子宫腺肌症(adenomyosis,AM)患者子宫肌层平滑肌组织中缩宫素受体(oxytocin receptor,OTR)的表达水平与痛经程度的关联性。方法收集2013年9月-2014年8月在本院妇科因AM行全子宫切除手术的腺肌症患者50例为腺肌症组,同期因子宫...目的探讨子宫腺肌症(adenomyosis,AM)患者子宫肌层平滑肌组织中缩宫素受体(oxytocin receptor,OTR)的表达水平与痛经程度的关联性。方法收集2013年9月-2014年8月在本院妇科因AM行全子宫切除手术的腺肌症患者50例为腺肌症组,同期因子宫肌瘤行全子宫切除的患者20例为对照组。应用免疫组化染色法(S-P法)检测20例正常子宫肌层、50例子宫腺肌症肌层平滑肌组织中的OTR表达情况。采用光学显微镜及Image Plus Pro 6.0图像分析系统测定OTR水平,并对其差异进行分析。结果 AM组子宫肌层平滑肌组织中OTR的表达高于对照组(62 370±34 758 vs 16 082±11 125,P<0.01),且表达强度随着痛经程度的加重而增加,差异均有统计学意义。结论 OTR在AM患者子宫肌层平滑肌组织中的表达与痛经程度呈正相关,推测患者痛经形式可能为宫缩痛,表明宫缩强度的增加参与了AM疼痛的发病机制。展开更多
Background: The myometrium at the location of the CS (caesarean section) scars, also known as residual myometrium thickness (RMT), is larger after a double-layer uterine closure procedure than following a single-layer...Background: The myometrium at the location of the CS (caesarean section) scars, also known as residual myometrium thickness (RMT), is larger after a double-layer uterine closure procedure than following a single-layer one. It may lessen the formation of a niche that is the myometrium’s disruption at the location of the scar of the uterus. Gynecological manifestations, obstetric problems in a future pregnancy and birth, and maybe subfertility are linked to thin RMT and a niche. Objective: To ascertain if double-layer unlocked closure of the uterus is better than single-layer one in terms of post-menstrual spotting and niche development following a first CS. Patients and Methods: In this randomized clinical study, 287 patients were evaluated for qualifying. Of all eligible individuals, 57 patients were excluded from the study based on the inclusion criteria. Results: The variation in ages, gestational age, body mass index (BMI), and cesarean section indications between the two assigned groups is statistically insignificant. However, postmenstrual spotting was statistically significantly more common in single-layer group compared to in double-group. The current study revealed ultrasound findings suggestive of niche formation was statistically significantly more common in single-layer group compared to in double-layer group. Conclusion: As evident from the current study, it demonstrates the advantages of double-layer unlocked closure of the uterus over single-layer one in terms of post-menstrual spotting and niche development following first-time cs. Thus, we deduced that fewer niches are formed, and fewer menstrual spotting occurs in the presence of double unlocked layers closure. To ascertain the impact of uterus closure method on post-operative niche development and the risk of obstetrics and gynaecological problems, further prospective trials with extended follow-up periods are required.展开更多
文摘目的探讨RhoA及Rho激酶（ROCKI）在子宫腺肌病患者子宫内膜一肌层交界区平滑肌细胞中的表达及其与痛经程度的关系。方法选择2012年3至12月在首都医科大学附属北京妇产医院妇科微创中心因子宫腺肌病行子宫全切除术的32例患者为子宫腺肌病组（其中增殖期内膜18例，分泌期内膜14例）；同期因宫颈病变或卵巢肿瘤而行子宫切除的患者29例为对照组（其中增殖期内膜12例，分泌期内膜17例）。对两组患者子宫内膜一肌层交界区平滑肌细胞进行分离及原代培养。应用实时荧光定量PCR技术和蛋白印迹法，检测两组及子宫腺肌病组不同程度痛经患者子宫内膜一肌层交界区平滑肌细胞中RhoA和ROCK I mRNA及其蛋白的表达水平。结果（1）子宫腺肌病组子宫内膜一肌层交界区平滑肌细胞中RhoA和ROCKImRNA表达水平无明显周期性变化，增殖期表达水平（分别为1．41±0．16、1．05±0．15）稍高于分泌期（分别为1．17±0．25、0．98±0．10），分别比较，差异均无统计学意义（P〉0．05）；对照组RhoA和ROCKImRNA表达水平存在周期性变化，增殖期表达水平（分别为0．93±0．10、1．00±0．18）高于分泌期（0．48±0．03、0．55±0．05），分别比较，差异均有统计学意义（P〈0．05）；子宫腺肌病组增殖期和分泌期RhoA和ROCKImRNA的表达水平均高于对照组，分别比较，差异均有统计学意义（P〈0．01）。（2）子宫腺肌病组与对照组RhoA及ROCKImRNA、蛋白表达水平均呈正相关关系（r=0．67，P〈0．01；r=0．48，P〈0．01）。（3）RhoA和ROCKImRNA、蛋白表达水平在子宫腺肌病组不同程度痛经患者中依次表现为：重度痛经（1．66±0．19、1．32±0．11）〉中度痛经（1．28±0．12、1．09±0．08）〉轻度痛经（0．93±0．09、0．81±0．06），两两分别比较，差异均有统计学意义（P〈0．05）。结论子宫腺肌病患者子
文摘目的探讨子宫腺肌症(adenomyosis,AM)患者子宫肌层平滑肌组织中缩宫素受体(oxytocin receptor,OTR)的表达水平与痛经程度的关联性。方法收集2013年9月-2014年8月在本院妇科因AM行全子宫切除手术的腺肌症患者50例为腺肌症组,同期因子宫肌瘤行全子宫切除的患者20例为对照组。应用免疫组化染色法(S-P法)检测20例正常子宫肌层、50例子宫腺肌症肌层平滑肌组织中的OTR表达情况。采用光学显微镜及Image Plus Pro 6.0图像分析系统测定OTR水平,并对其差异进行分析。结果 AM组子宫肌层平滑肌组织中OTR的表达高于对照组(62 370±34 758 vs 16 082±11 125,P<0.01),且表达强度随着痛经程度的加重而增加,差异均有统计学意义。结论 OTR在AM患者子宫肌层平滑肌组织中的表达与痛经程度呈正相关,推测患者痛经形式可能为宫缩痛,表明宫缩强度的增加参与了AM疼痛的发病机制。
文摘Background: The myometrium at the location of the CS (caesarean section) scars, also known as residual myometrium thickness (RMT), is larger after a double-layer uterine closure procedure than following a single-layer one. It may lessen the formation of a niche that is the myometrium’s disruption at the location of the scar of the uterus. Gynecological manifestations, obstetric problems in a future pregnancy and birth, and maybe subfertility are linked to thin RMT and a niche. Objective: To ascertain if double-layer unlocked closure of the uterus is better than single-layer one in terms of post-menstrual spotting and niche development following a first CS. Patients and Methods: In this randomized clinical study, 287 patients were evaluated for qualifying. Of all eligible individuals, 57 patients were excluded from the study based on the inclusion criteria. Results: The variation in ages, gestational age, body mass index (BMI), and cesarean section indications between the two assigned groups is statistically insignificant. However, postmenstrual spotting was statistically significantly more common in single-layer group compared to in double-group. The current study revealed ultrasound findings suggestive of niche formation was statistically significantly more common in single-layer group compared to in double-layer group. Conclusion: As evident from the current study, it demonstrates the advantages of double-layer unlocked closure of the uterus over single-layer one in terms of post-menstrual spotting and niche development following first-time cs. Thus, we deduced that fewer niches are formed, and fewer menstrual spotting occurs in the presence of double unlocked layers closure. To ascertain the impact of uterus closure method on post-operative niche development and the risk of obstetrics and gynaecological problems, further prospective trials with extended follow-up periods are required.
