AIM To study the effects of psoralen (PSO) and 8 methoxypsoralen (8 MOP) on the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells. METHODS Inhibitory effects of PSO and 8 MOP on the prolifer...AIM To study the effects of psoralen (PSO) and 8 methoxypsoralen (8 MOP) on the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells. METHODS Inhibitory effects of PSO and 8 MOP on the proliferation and metastatic potential of mucoepidermaid carcinoma Mc3 cells were investigated with MTT assay, cell counting, flow cytometry and tail vein injection of the cells into nude mice (10 6 for each). RESULTS PSO and 8 MOP inhibited Mc3 cell growth in a dose dependent way. The IC 30 (mg·L -1 ) of PSO and 8 MOP were 32.4 and 25.1 and IC 50 (mg·L -1 ) 44.7 and 35.5 respectively. After the cells had been treated with the drugs at IC 30 for 5 d, the doubling time (h) for the control, PSO treated and 8 MOP treated cells were 20.8, 23.3 and 57.8 respectively, the percentage of S phase cells 24.5,17.8 and 5.8, the wild type p53 expression (%) 24.0,99.8 and 99.0, the nm23 H 1 expression (%) 99 9,99.5 and 99.1, the clonogenesity (%) 23.5,16.5 and 0, the number of metastatic foci on lung surface 139±61,114±68 and 36±32, respectively. CONCLUSION Both PSO and 8 MOP at the dosage of IC 30 may inhibit the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells, but 8 MOP is more effective.展开更多
Background： Tracheobronchial mucoepidermoid carcinoma （MEC） is a rare airway tumor in adults for which surgery is considered a first-line treatment. However, some patients already lost the best opportunity of a sur...Background： Tracheobronchial mucoepidermoid carcinoma （MEC） is a rare airway tumor in adults for which surgery is considered a first-line treatment. However, some patients already lost the best opportunity of a surgical intervention when diagnoses are confirmed, and surgery causes considerable trauma resulting in partial loss of pulmonary function. Moreover, the tumor is resistant to radiotherapy and chemotherapy. These factors make the treatment of tracheobronchial MEC challenging. This study aimed to evaluate the safety and et^cacy ofinterventional bronchoscopic therapy in adult patients with tracheobronchial MEC. Methods： We retrospectively analyzed the clinical manifestations, bronchoscopic interventions, complications, and outcomes of 11 adult patients with tracheobronchial MEC. Paired t-test was used to analyze the parameters of the American Thoracic Society Dyspnea Index and the Karnofsky Score before and after the first interventional bronchoscopic therapy. Results： All tumors occurred in the main bronchus and were easily visualized by bronchoscopy. After interventional bronchoscopic therapy, the symptoms of all patients showed significant improvement. The American Thoracic Society Dyspnea Index decreased from 1.91 ± 1.22 to 0.27 ± 0.47 （t = 6.708, P 〈 0.001） and the Kamofsky Score increased from 78.18 ±16.62 to 95.46 ± 8.20 （t =-5.190, P 〈 0.001 ）. Bronchoscopic intervention did not result in serious complications or mortality. During the follow-up period between 3 and 96 months after the first therapy, the following results were noted： （ 1 ） among the eight patients with low-grade tracheobronchial MEC, only one patient had a relapse and agreed to surgical treatment; （2） among the three patients with high-grade tracheobronchial MEC, one patient required repeated bronchoscopic interventions, one patient died of pulmonary infection, and one patient died of systemic failure owing to tumor metastasis. Conclusions： Interventional bronchoscopic therapy, as an alternative t展开更多
文摘AIM To study the effects of psoralen (PSO) and 8 methoxypsoralen (8 MOP) on the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells. METHODS Inhibitory effects of PSO and 8 MOP on the proliferation and metastatic potential of mucoepidermaid carcinoma Mc3 cells were investigated with MTT assay, cell counting, flow cytometry and tail vein injection of the cells into nude mice (10 6 for each). RESULTS PSO and 8 MOP inhibited Mc3 cell growth in a dose dependent way. The IC 30 (mg·L -1 ) of PSO and 8 MOP were 32.4 and 25.1 and IC 50 (mg·L -1 ) 44.7 and 35.5 respectively. After the cells had been treated with the drugs at IC 30 for 5 d, the doubling time (h) for the control, PSO treated and 8 MOP treated cells were 20.8, 23.3 and 57.8 respectively, the percentage of S phase cells 24.5,17.8 and 5.8, the wild type p53 expression (%) 24.0,99.8 and 99.0, the nm23 H 1 expression (%) 99 9,99.5 and 99.1, the clonogenesity (%) 23.5,16.5 and 0, the number of metastatic foci on lung surface 139±61,114±68 and 36±32, respectively. CONCLUSION Both PSO and 8 MOP at the dosage of IC 30 may inhibit the proliferation and metastatic potential of mucoepidermoid carcinoma Mc3 cells, but 8 MOP is more effective.
文摘Background： Tracheobronchial mucoepidermoid carcinoma （MEC） is a rare airway tumor in adults for which surgery is considered a first-line treatment. However, some patients already lost the best opportunity of a surgical intervention when diagnoses are confirmed, and surgery causes considerable trauma resulting in partial loss of pulmonary function. Moreover, the tumor is resistant to radiotherapy and chemotherapy. These factors make the treatment of tracheobronchial MEC challenging. This study aimed to evaluate the safety and et^cacy ofinterventional bronchoscopic therapy in adult patients with tracheobronchial MEC. Methods： We retrospectively analyzed the clinical manifestations, bronchoscopic interventions, complications, and outcomes of 11 adult patients with tracheobronchial MEC. Paired t-test was used to analyze the parameters of the American Thoracic Society Dyspnea Index and the Karnofsky Score before and after the first interventional bronchoscopic therapy. Results： All tumors occurred in the main bronchus and were easily visualized by bronchoscopy. After interventional bronchoscopic therapy, the symptoms of all patients showed significant improvement. The American Thoracic Society Dyspnea Index decreased from 1.91 ± 1.22 to 0.27 ± 0.47 （t = 6.708, P 〈 0.001） and the Kamofsky Score increased from 78.18 ±16.62 to 95.46 ± 8.20 （t =-5.190, P 〈 0.001 ）. Bronchoscopic intervention did not result in serious complications or mortality. During the follow-up period between 3 and 96 months after the first therapy, the following results were noted： （ 1 ） among the eight patients with low-grade tracheobronchial MEC, only one patient had a relapse and agreed to surgical treatment; （2） among the three patients with high-grade tracheobronchial MEC, one patient required repeated bronchoscopic interventions, one patient died of pulmonary infection, and one patient died of systemic failure owing to tumor metastasis. Conclusions： Interventional bronchoscopic therapy, as an alternative t
