目的·分析线粒体核糖体蛋白L12(mitochondrial ribosomal protein L12,MRPL12)在肺腺癌(lung adenocarcinoma,LUAD)和正常组织中的表达差异和对患者预后的影响,以及预测其生物学功能。方法·利用肿瘤浸润性免疫细胞分析数据库T...目的·分析线粒体核糖体蛋白L12(mitochondrial ribosomal protein L12,MRPL12)在肺腺癌(lung adenocarcinoma,LUAD)和正常组织中的表达差异和对患者预后的影响,以及预测其生物学功能。方法·利用肿瘤浸润性免疫细胞分析数据库TIMER和GEPIA(gene expression profiling and interactive analyses)交互式网站服务器分析癌症基因组图谱(The Cancer Genome Atlas,TCGA)中MRPL12在不同癌组织中的表达情况的影响。利用Sangerbox生物信息分析平台对MRPL12差异表达的癌症类型进行Cox回归和Kaplan-Meier生存分析,评估风险比(hazard ratio,HR)、95%置信区间(confidence interval,CI)和Log-rank P值。采用UALCAN数据分析平台评估MRPL12蛋白表达与临床病理参数的关系。使用LinkedOmics数据库筛选与MRPL12表达相关的基因。采用DAVID数据库进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。定量聚合酶链反应验证MRPL12在细胞中的转录水平。结果·MRPL12在多种癌组织中显著高表达,但只有在LUAD中MRPL12的高表达与患者较差的总体生存期(overall suivival,OS)和疾病特异性生存期(disease-specific survival,DSS)相关;肿瘤分级程度越高的LUAD患者MRPL12蛋白表达水平越高;在LUAD基因表达数据集中与MRPL12负相关的基因富集到癌症通路(P=0.000)、癌症蛋白聚糖(P=0.000)、非小细胞肺癌(P=0.000)等癌症相关的通路。MRPL12在LUAD细胞(H1395、H1975和HCC827)中的mRNA表达水平高于正常细胞(Beas-2B),差异有统计学意义(P<0.05)。结论·MRPL12高表达的LUAD患者可能预后较差;MRPL12可以作为LUAD预后不良的潜在生物标志物和潜在的治疗新靶点。展开更多
BACKGROUND Gastric carcinoma(GC)is a digestive system disease with high morbidity and mortality.However,early clinical detection is difficult,and the therapeutic effect for advanced disease is not satisfactory.Thus,fi...BACKGROUND Gastric carcinoma(GC)is a digestive system disease with high morbidity and mortality.However,early clinical detection is difficult,and the therapeutic effect for advanced disease is not satisfactory.Thus,finding new tumor markers and therapeutic targets conducive to the treatment of GC is imperative.MRPL35 is a member of the large subunit family of mitochondrial ribosomal protein.MRPL35 shows the characteristic of oncogene in colorectal cancer and esophageal cancer,which promotes the exploration of the correlation between MRPL35 and GC.We proposed that the expression of MRPL35 might be critical in GC.AIM To study the effect of MRPL35 knockdown on GC cell proliferation.METHODS The expression of MRPL35 in GC was evaluated based on data from the publictumor database UALCAN(www.ualcan.path.uab.edu).The effect of theexpression of MRPL35 on the prognosis was evaluated with KMplot(www.kmplot.com).The expression of MRPL35 was assessed on the tissuemicroarray by immunohistochemistry and the level of MRPL35 mRNA in 25 pairsof clinical GC tissues and matched adjacent tissues was detected by quantitativereverse transcription-polymerase chain reaction.Celigo cell count assay,colonyformation assay,and flow cytometry were used to assess the role of MRPL35 inGC cell proliferation and apoptosis in vitro.Additionally,tumor formationexperiment in BALB/c nude mice was utilized to determine the effect of MRPL35on GC cell proliferation.After knockdown of MRPL35,related proteins wereidentified by isobaric tags for relative and absolute quantification analysis,andthe expression of related proteins was detected by Western blot.RESULTSThe expression of MRPL35 was up-regulated in GC(P=1.77×10^(-4)).The Kaplan-Meier plots of the overall survival indicated that high expression of MRPL35 wasassociated with a poor survival in GC.Compared with adjacent tissues,theexpression of MRPL35 in GC tissues was increased,which was related to age(P=0.03),lymph node metastasis(P=0.007),and pathological tumor-node-metastasisstage(P=0.024).Knockd展开更多
文摘目的·分析线粒体核糖体蛋白L12(mitochondrial ribosomal protein L12,MRPL12)在肺腺癌(lung adenocarcinoma,LUAD)和正常组织中的表达差异和对患者预后的影响,以及预测其生物学功能。方法·利用肿瘤浸润性免疫细胞分析数据库TIMER和GEPIA(gene expression profiling and interactive analyses)交互式网站服务器分析癌症基因组图谱(The Cancer Genome Atlas,TCGA)中MRPL12在不同癌组织中的表达情况的影响。利用Sangerbox生物信息分析平台对MRPL12差异表达的癌症类型进行Cox回归和Kaplan-Meier生存分析,评估风险比(hazard ratio,HR)、95%置信区间(confidence interval,CI)和Log-rank P值。采用UALCAN数据分析平台评估MRPL12蛋白表达与临床病理参数的关系。使用LinkedOmics数据库筛选与MRPL12表达相关的基因。采用DAVID数据库进行京都基因与基因组百科全书(Kyoto Encyclopedia of Genes and Genomes,KEGG)通路富集分析。定量聚合酶链反应验证MRPL12在细胞中的转录水平。结果·MRPL12在多种癌组织中显著高表达,但只有在LUAD中MRPL12的高表达与患者较差的总体生存期(overall suivival,OS)和疾病特异性生存期(disease-specific survival,DSS)相关;肿瘤分级程度越高的LUAD患者MRPL12蛋白表达水平越高;在LUAD基因表达数据集中与MRPL12负相关的基因富集到癌症通路(P=0.000)、癌症蛋白聚糖(P=0.000)、非小细胞肺癌(P=0.000)等癌症相关的通路。MRPL12在LUAD细胞(H1395、H1975和HCC827)中的mRNA表达水平高于正常细胞(Beas-2B),差异有统计学意义(P<0.05)。结论·MRPL12高表达的LUAD患者可能预后较差;MRPL12可以作为LUAD预后不良的潜在生物标志物和潜在的治疗新靶点。
文摘BACKGROUND Gastric carcinoma(GC)is a digestive system disease with high morbidity and mortality.However,early clinical detection is difficult,and the therapeutic effect for advanced disease is not satisfactory.Thus,finding new tumor markers and therapeutic targets conducive to the treatment of GC is imperative.MRPL35 is a member of the large subunit family of mitochondrial ribosomal protein.MRPL35 shows the characteristic of oncogene in colorectal cancer and esophageal cancer,which promotes the exploration of the correlation between MRPL35 and GC.We proposed that the expression of MRPL35 might be critical in GC.AIM To study the effect of MRPL35 knockdown on GC cell proliferation.METHODS The expression of MRPL35 in GC was evaluated based on data from the publictumor database UALCAN(www.ualcan.path.uab.edu).The effect of theexpression of MRPL35 on the prognosis was evaluated with KMplot(www.kmplot.com).The expression of MRPL35 was assessed on the tissuemicroarray by immunohistochemistry and the level of MRPL35 mRNA in 25 pairsof clinical GC tissues and matched adjacent tissues was detected by quantitativereverse transcription-polymerase chain reaction.Celigo cell count assay,colonyformation assay,and flow cytometry were used to assess the role of MRPL35 inGC cell proliferation and apoptosis in vitro.Additionally,tumor formationexperiment in BALB/c nude mice was utilized to determine the effect of MRPL35on GC cell proliferation.After knockdown of MRPL35,related proteins wereidentified by isobaric tags for relative and absolute quantification analysis,andthe expression of related proteins was detected by Western blot.RESULTSThe expression of MRPL35 was up-regulated in GC(P=1.77×10^(-4)).The Kaplan-Meier plots of the overall survival indicated that high expression of MRPL35 wasassociated with a poor survival in GC.Compared with adjacent tissues,theexpression of MRPL35 in GC tissues was increased,which was related to age(P=0.03),lymph node metastasis(P=0.007),and pathological tumor-node-metastasisstage(P=0.024).Knockd
