In order to effectively protect the activity of Epigallocatechin gallate（EGCG）, we explored the protection mechanism of Konjac glucomannan（KGM） for EGCG by experiments and theory analyses. We synthesized KGM/EGCG ...In order to effectively protect the activity of Epigallocatechin gallate（EGCG）, we explored the protection mechanism of Konjac glucomannan（KGM） for EGCG by experiments and theory analyses. We synthesized KGM/EGCG nanofibers by using electrostatic spinning method. The microstructure of nanofibers was characterized by SEM, FTIR, TGA, XRD and Raman spectroscopic. The formation mechanism and the protection effects of KGM/EGCG nanofibers were also discussed. The results showed that the EGCG activity was protected due to the hydrogen bonds between-OH of EGCG and KGM, and EGCG was embedded in KGM nanofiber with bead style. The reducing force and DPPH scavenging ability data indicated that KGM/EGCG nanofibers have stronger antioxidant activity than the EGCG solution under the same condition. Hence, the mosaic topological structure of KGM can effectively extend the EGCG activity.展开更多
Cucumber green mottle mosaic virus (CGMMV), a member of the Tobamovirus genus, causes a severe disease of cucurbits. In the Moscow region of Russian Federation, the incidence of infection on cucumber plants in greenho...Cucumber green mottle mosaic virus (CGMMV), a member of the Tobamovirus genus, causes a severe disease of cucurbits. In the Moscow region of Russian Federation, the incidence of infection on cucumber plants in greenhouses is high;however, the virus is poorly studied. In this work, the full-length genomes of two pathogenic MC-1 and MC-2 strains of CGMMV isolated from cucumber plants grown in greenhouses in the Moscow region and the attenuated VIROG-43M strain were sequenced. Comparison of VIROG-43M nucleotide sequence with those of the pathogenic strains revealed three missense mutations. Their role in attenuation is discussed. For the first time, in a number of trials conducted under laboratory conditions and in commercial greenhouses, the efficiency of the attenuated VIROG-43M strain as a biocontrol agent for cucumber plant protection resulting in significant yield gain was demonstrated. Phylogenetic analysis with 83 full-length CGMMV coat protein genes isolated in 16 different countries showed that Russian strains are related to isolates from Spain, Greece, USA and Israel.展开更多
基金supported by the National Natural Science Foundation of China(31471704,31772045)
文摘In order to effectively protect the activity of Epigallocatechin gallate（EGCG）, we explored the protection mechanism of Konjac glucomannan（KGM） for EGCG by experiments and theory analyses. We synthesized KGM/EGCG nanofibers by using electrostatic spinning method. The microstructure of nanofibers was characterized by SEM, FTIR, TGA, XRD and Raman spectroscopic. The formation mechanism and the protection effects of KGM/EGCG nanofibers were also discussed. The results showed that the EGCG activity was protected due to the hydrogen bonds between-OH of EGCG and KGM, and EGCG was embedded in KGM nanofiber with bead style. The reducing force and DPPH scavenging ability data indicated that KGM/EGCG nanofibers have stronger antioxidant activity than the EGCG solution under the same condition. Hence, the mosaic topological structure of KGM can effectively extend the EGCG activity.
文摘Cucumber green mottle mosaic virus (CGMMV), a member of the Tobamovirus genus, causes a severe disease of cucurbits. In the Moscow region of Russian Federation, the incidence of infection on cucumber plants in greenhouses is high;however, the virus is poorly studied. In this work, the full-length genomes of two pathogenic MC-1 and MC-2 strains of CGMMV isolated from cucumber plants grown in greenhouses in the Moscow region and the attenuated VIROG-43M strain were sequenced. Comparison of VIROG-43M nucleotide sequence with those of the pathogenic strains revealed three missense mutations. Their role in attenuation is discussed. For the first time, in a number of trials conducted under laboratory conditions and in commercial greenhouses, the efficiency of the attenuated VIROG-43M strain as a biocontrol agent for cucumber plant protection resulting in significant yield gain was demonstrated. Phylogenetic analysis with 83 full-length CGMMV coat protein genes isolated in 16 different countries showed that Russian strains are related to isolates from Spain, Greece, USA and Israel.
