Objective:To observe dynamic changes of levels of monocyte chemotactic protein-1(MCP-1),tumor necrosis factor-α(TNF-α) and interleukin-8(IL-8) in patients with acute pancreatitis and to investigate its evaluation va...Objective:To observe dynamic changes of levels of monocyte chemotactic protein-1(MCP-1),tumor necrosis factor-α(TNF-α) and interleukin-8(IL-8) in patients with acute pancreatitis and to investigate its evaluation value on the severity of acute pancreatitis.Methods:A total of 109 patients with acute pancreatitis admitted were divided into mild acute pancreatitis group(MAP group,42 cases),moderately severe acute pancreatitis(MSAP group,35 cases)and severe acute pancreatitis(SAP group,32 cases).ELISA was used to detect the serum levels of MCP-1,TNF-α and IL-8 of patients at day 1,day 4 and day 7 of admission to hospital.Results:The serum levels of MCP-1,TNF-α and IL-8 from MAP group,MSAP group and SAP group at day 1 of admission to hospital all significantly increased.There was a significant difference between MAP group and control group,MSAP group and MAP group,SAP group and MSAP group(P<0.05).The serum concentrations of IL-8 from MASP group and SAP group obviously increased at day 1,and there was significant difference between MASP group and MAP group,SAP group and MSAP group(P<0.05),while the difference between MAP group and control group was not obvious(P>0.05);The serum concentrations of MCP-1,TNF-α and IL-8 from MAP group all reached the highest level at day 4,which were significantly higher than the detection levels at day 1.In MSAP group and SAP group,the serum concentrations of MCP-1,TNF-α and IL-8 were the highest at day 1,which were significantly higher than the detection levels at day 4 and 7.At each detecting timing,the serum concentrations of MCP-1,TNF-α and IL-8 from MSAP group and SAP group were all higher than those of MAP group and MSAP group,respectively.Conclusions:The dynamic changes of serum levels of MCP-1,TNF-α and IL-8 in patients with acute pancreatitis have their rules,and the change rule of MAP group was different with that of MSAP and SAP group,which showed the reference value for the diagnosis and illness severity evaluation of acute pancreatitis.展开更多
BACKGROUND: Chemokines and their receptors play key roles in the pathogenesis of acute pancreatitis. This study aimed to establish a rat model of severe acute pancreatitis (SAP) for investigating monocyte chemotactic ...BACKGROUND: Chemokines and their receptors play key roles in the pathogenesis of acute pancreatitis. This study aimed to establish a rat model of severe acute pancreatitis (SAP) for investigating monocyte chemotactic protein-1 (MCP-1) expression in the pathogenesis of the disease. We assessed the effects of the inhibitor of MCP-1, Bindarit, on SAP and explored the mechanisms underlying SAP. METHODS: Seventy-two Sprague-Dawley rats were randomly divided into a saline control group (group S), an SAP group (group P), and a Bindarit group (group T). The SAP model was induced by retrograde infusion of 4% sodium taurocholate into the bilio-pancreatic duct. Based on the SAP model, Bindarit was injected intraperitoneally in group T, and 0.5% methyl cellulose was injected intraperitoneally in groups S and P. In group S, saline was retrogradely infused into the bilpancreatic duct. Serum amylase levels and the histological changes in the pancreas were assessed at different time-points in each group. Expression of MCP-1 in serum was measured by enzyme-linked immunoadsorbent assay (ELISA). MCP-1 protein and mRNA expression levels were detected by immunohistochemistry, Western blotting, and semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: Serum amylase levels in groups P and T were higher than those in group S. Serum amylase levels were significantly lower in group T than in group P at 6 and 12 hours after operation. The levels of MCP-1 in serum at 6 and 12 hours after operation in group P were significantly higher than in group S, and significantly lower in group T than in group P at 6 and 12 hours after operation. The pathological damage in the pancreas was milder in group T than in group P. MCP-1 protein and mRNA expression levels in the pancreas were higher in groups P and T than in group S. These expression levels were positively correlated with the pathological damage of pancreatic tissues. The activity of MCP-1 in group T was significantly lower than in group P. CONCLUSION: MCP-1 may 展开更多
背景急性缺血性脑卒中(AIS)为临床常见急危重症,且其发病率逐渐升高。近年来AIS的诊治水平明显提高,但对患者预后的评估还缺少准确、安全和简便的方法。因而寻找可早期用于诊断及病情评估的实验室指标对临床治疗方案的制定及患者预后的...背景急性缺血性脑卒中(AIS)为临床常见急危重症,且其发病率逐渐升高。近年来AIS的诊治水平明显提高,但对患者预后的评估还缺少准确、安全和简便的方法。因而寻找可早期用于诊断及病情评估的实验室指标对临床治疗方案的制定及患者预后的改善至关重要。目的探究AIS患者炎性因子水平、血压变异性(BPV)与病情严重程度及预后的相关性。方法选取2017年12月—2019年12月于大冶市人民医院神经内科接受治疗的AIS患者106例为研究对象。收集患者一般资料、炎性因子〔细胞间黏附分子1(ICAM-1)、单核细胞趋化蛋白1(MCP-1)〕水平、BPV指标〔包括白昼收缩压标准差(dSSD)、白昼舒张压标准差(dDSD)、夜间收缩压标准差(nSSD)、夜间舒张压标准差(nDSD)、24 h收缩压标准差(24 h SSD)、24 h舒张压标准差(24 h DSD)〕。根据美国国立卫生研究院卒中量表(NIHSS)评分将患者分为轻型组(<4分,37例)、中型组(4~15分,45例)、重型组(>15分,24例)。根据改良Rankin量表(mRS)评分将患者分为预后良好组(0~2分,71例)及预后不良组(>2分,35例)。采用Spearman秩相关分析探讨AIS患者炎性因子水平、BPV指标与病情严重程度、预后的相关性。结果中型组、重型组患者ICAM-1、MCP-1水平及dSSD、dDSD、nDSD、24 h SSD、24 h DSD高于轻型组(P<0.05);重型组患者ICAM-1、MCP-1水平及24 h SSD高于中型组(P<0.05)。Spearman秩相关分析结果显示,AIS患者ICAM-1、MCP-1水平及24 h SSD与病情严重程度呈正相关(rs值分别为0.657、0.598、0.527,P值均<0.001)。预后不良组患者年龄、有吸烟史者所占比例、高血压发生率高于预后良好组(P<0.05)。预后不良组患者ICAM-1、MCP-1水平及dSSD、dDSD、24 h SSD、24 h DSD高于预后良好组(P<0.05)。Spearman秩相关分析结果显示,AIS患者ICAM-1、MCP-1水平及dSSD、dDSD、24 h SSD、24 h DSD与预后呈正相关(rs值分别为0.601、0.652、0.571、0.458、0.523、0.展开更多
目的探讨慢性阻塞性肺疾病(简称慢阻肺)伴或不伴低氧血症患者血清单核细胞趋化蛋白-1(MCP-1)、血清淀粉样蛋白A(SAA)水平与认知功能的关系,以期为慢阻肺患者筛查预测早期认知功能损伤的血清学指标,同时探讨低氧血症在认知障碍发生中的...目的探讨慢性阻塞性肺疾病(简称慢阻肺)伴或不伴低氧血症患者血清单核细胞趋化蛋白-1(MCP-1)、血清淀粉样蛋白A(SAA)水平与认知功能的关系,以期为慢阻肺患者筛查预测早期认知功能损伤的血清学指标,同时探讨低氧血症在认知障碍发生中的作用。方法纳入2013年1月至2017年1月于华北理工大学附属医院住院的62例慢阻肺患者,依据血气分析将其分为慢阻肺缺氧组(25例)和慢阻肺无缺氧组(37例),同期30例健康体检人群为对照组。采用酶联免疫吸附法测定慢阻肺患者及对照人群血清MCP-1、SAA浓度,逆转录聚合酶链反应检测外周血单核细胞中MCP-1、SAA的m RNA表达水平,应用蒙特利尔认知评估量表(Mo CA量表)进行认知功能测定。分析三组人群在血清MCP-1、SAA水平及外周血MCP-1 m RNA、SAA m RNA水平表达有无差异及与认知功能损伤的关系。结果血清MCP-1、SAA水平及外周血单核细胞MCP-1 m RNA、SAA m RNA水平表达水平趋势一致,慢阻肺患者血清MCP-1、SAA水平及外周血单核细胞MCP-1 m RNA、SAA m RNA水平高于健康对照组,差异有统计学意义(均P<0.05);慢阻肺缺氧组和慢阻肺无缺氧组Mo CA评分总分较对照组低,其中缺氧组Mo CA评分降低更明显,差异有统计学意义(均P<0.05)。血清SAA、MCP-1水平及外周血单核细胞MCP-1 m RNA、SAA m RNA水平与Mo CA总分呈负相关(均P<0.05)。结论慢阻肺患者血清SAA、MCP-1水平及外周血单核细胞MCP-1 m RNA、SAA m RNA水平高低与患者认知障碍程度呈正相关,低氧血症可能是慢阻肺患者发生认知功能受损的因素之一。展开更多
基金supported by Health and Family Planning Commission of Hainan Province,China,Scientific Research Project(Grant No.14A210207)
文摘Objective:To observe dynamic changes of levels of monocyte chemotactic protein-1(MCP-1),tumor necrosis factor-α(TNF-α) and interleukin-8(IL-8) in patients with acute pancreatitis and to investigate its evaluation value on the severity of acute pancreatitis.Methods:A total of 109 patients with acute pancreatitis admitted were divided into mild acute pancreatitis group(MAP group,42 cases),moderately severe acute pancreatitis(MSAP group,35 cases)and severe acute pancreatitis(SAP group,32 cases).ELISA was used to detect the serum levels of MCP-1,TNF-α and IL-8 of patients at day 1,day 4 and day 7 of admission to hospital.Results:The serum levels of MCP-1,TNF-α and IL-8 from MAP group,MSAP group and SAP group at day 1 of admission to hospital all significantly increased.There was a significant difference between MAP group and control group,MSAP group and MAP group,SAP group and MSAP group(P<0.05).The serum concentrations of IL-8 from MASP group and SAP group obviously increased at day 1,and there was significant difference between MASP group and MAP group,SAP group and MSAP group(P<0.05),while the difference between MAP group and control group was not obvious(P>0.05);The serum concentrations of MCP-1,TNF-α and IL-8 from MAP group all reached the highest level at day 4,which were significantly higher than the detection levels at day 1.In MSAP group and SAP group,the serum concentrations of MCP-1,TNF-α and IL-8 were the highest at day 1,which were significantly higher than the detection levels at day 4 and 7.At each detecting timing,the serum concentrations of MCP-1,TNF-α and IL-8 from MSAP group and SAP group were all higher than those of MAP group and MSAP group,respectively.Conclusions:The dynamic changes of serum levels of MCP-1,TNF-α and IL-8 in patients with acute pancreatitis have their rules,and the change rule of MAP group was different with that of MSAP and SAP group,which showed the reference value for the diagnosis and illness severity evaluation of acute pancreatitis.
基金supported by grants from the social Burteall Foundation of Nantong(S5054)
文摘BACKGROUND: Chemokines and their receptors play key roles in the pathogenesis of acute pancreatitis. This study aimed to establish a rat model of severe acute pancreatitis (SAP) for investigating monocyte chemotactic protein-1 (MCP-1) expression in the pathogenesis of the disease. We assessed the effects of the inhibitor of MCP-1, Bindarit, on SAP and explored the mechanisms underlying SAP. METHODS: Seventy-two Sprague-Dawley rats were randomly divided into a saline control group (group S), an SAP group (group P), and a Bindarit group (group T). The SAP model was induced by retrograde infusion of 4% sodium taurocholate into the bilio-pancreatic duct. Based on the SAP model, Bindarit was injected intraperitoneally in group T, and 0.5% methyl cellulose was injected intraperitoneally in groups S and P. In group S, saline was retrogradely infused into the bilpancreatic duct. Serum amylase levels and the histological changes in the pancreas were assessed at different time-points in each group. Expression of MCP-1 in serum was measured by enzyme-linked immunoadsorbent assay (ELISA). MCP-1 protein and mRNA expression levels were detected by immunohistochemistry, Western blotting, and semi-quantitative reverse transcriptase-polymerase chain reaction (RT-PCR). RESULTS: Serum amylase levels in groups P and T were higher than those in group S. Serum amylase levels were significantly lower in group T than in group P at 6 and 12 hours after operation. The levels of MCP-1 in serum at 6 and 12 hours after operation in group P were significantly higher than in group S, and significantly lower in group T than in group P at 6 and 12 hours after operation. The pathological damage in the pancreas was milder in group T than in group P. MCP-1 protein and mRNA expression levels in the pancreas were higher in groups P and T than in group S. These expression levels were positively correlated with the pathological damage of pancreatic tissues. The activity of MCP-1 in group T was significantly lower than in group P. CONCLUSION: MCP-1 may
文摘背景急性缺血性脑卒中(AIS)为临床常见急危重症,且其发病率逐渐升高。近年来AIS的诊治水平明显提高,但对患者预后的评估还缺少准确、安全和简便的方法。因而寻找可早期用于诊断及病情评估的实验室指标对临床治疗方案的制定及患者预后的改善至关重要。目的探究AIS患者炎性因子水平、血压变异性(BPV)与病情严重程度及预后的相关性。方法选取2017年12月—2019年12月于大冶市人民医院神经内科接受治疗的AIS患者106例为研究对象。收集患者一般资料、炎性因子〔细胞间黏附分子1(ICAM-1)、单核细胞趋化蛋白1(MCP-1)〕水平、BPV指标〔包括白昼收缩压标准差(dSSD)、白昼舒张压标准差(dDSD)、夜间收缩压标准差(nSSD)、夜间舒张压标准差(nDSD)、24 h收缩压标准差(24 h SSD)、24 h舒张压标准差(24 h DSD)〕。根据美国国立卫生研究院卒中量表(NIHSS)评分将患者分为轻型组(<4分,37例)、中型组(4~15分,45例)、重型组(>15分,24例)。根据改良Rankin量表(mRS)评分将患者分为预后良好组(0~2分,71例)及预后不良组(>2分,35例)。采用Spearman秩相关分析探讨AIS患者炎性因子水平、BPV指标与病情严重程度、预后的相关性。结果中型组、重型组患者ICAM-1、MCP-1水平及dSSD、dDSD、nDSD、24 h SSD、24 h DSD高于轻型组(P<0.05);重型组患者ICAM-1、MCP-1水平及24 h SSD高于中型组(P<0.05)。Spearman秩相关分析结果显示,AIS患者ICAM-1、MCP-1水平及24 h SSD与病情严重程度呈正相关(rs值分别为0.657、0.598、0.527,P值均<0.001)。预后不良组患者年龄、有吸烟史者所占比例、高血压发生率高于预后良好组(P<0.05)。预后不良组患者ICAM-1、MCP-1水平及dSSD、dDSD、24 h SSD、24 h DSD高于预后良好组(P<0.05)。Spearman秩相关分析结果显示,AIS患者ICAM-1、MCP-1水平及dSSD、dDSD、24 h SSD、24 h DSD与预后呈正相关(rs值分别为0.601、0.652、0.571、0.458、0.523、0.
文摘目的探讨慢性阻塞性肺疾病(简称慢阻肺)伴或不伴低氧血症患者血清单核细胞趋化蛋白-1(MCP-1)、血清淀粉样蛋白A(SAA)水平与认知功能的关系,以期为慢阻肺患者筛查预测早期认知功能损伤的血清学指标,同时探讨低氧血症在认知障碍发生中的作用。方法纳入2013年1月至2017年1月于华北理工大学附属医院住院的62例慢阻肺患者,依据血气分析将其分为慢阻肺缺氧组(25例)和慢阻肺无缺氧组(37例),同期30例健康体检人群为对照组。采用酶联免疫吸附法测定慢阻肺患者及对照人群血清MCP-1、SAA浓度,逆转录聚合酶链反应检测外周血单核细胞中MCP-1、SAA的m RNA表达水平,应用蒙特利尔认知评估量表(Mo CA量表)进行认知功能测定。分析三组人群在血清MCP-1、SAA水平及外周血MCP-1 m RNA、SAA m RNA水平表达有无差异及与认知功能损伤的关系。结果血清MCP-1、SAA水平及外周血单核细胞MCP-1 m RNA、SAA m RNA水平表达水平趋势一致,慢阻肺患者血清MCP-1、SAA水平及外周血单核细胞MCP-1 m RNA、SAA m RNA水平高于健康对照组,差异有统计学意义(均P<0.05);慢阻肺缺氧组和慢阻肺无缺氧组Mo CA评分总分较对照组低,其中缺氧组Mo CA评分降低更明显,差异有统计学意义(均P<0.05)。血清SAA、MCP-1水平及外周血单核细胞MCP-1 m RNA、SAA m RNA水平与Mo CA总分呈负相关(均P<0.05)。结论慢阻肺患者血清SAA、MCP-1水平及外周血单核细胞MCP-1 m RNA、SAA m RNA水平高低与患者认知障碍程度呈正相关,低氧血症可能是慢阻肺患者发生认知功能受损的因素之一。
