Drugging the p53 pathway has been a goal for both academics and pharmaceutical companies since the designation of p53 as the'guardian of the genome'.Through growing understanding of p53 biology,we can see mult...Drugging the p53 pathway has been a goal for both academics and pharmaceutical companies since the designation of p53 as the'guardian of the genome'.Through growing understanding of p53 biology,we can see multiple routes for activation of both wild-type p53 function and restoration of mutant p53?In this review,we focus on small molecules that activate wild-type p53 and that do so in a nongenotoxit manner.In particular,we will describe potential approaches to targeting proteins that alter p53 stability and function through posttranslational modification,affect p53's subcellular localization,or target RNA synthesis or the synthesis of ribonucleotides.The plethora of pathways for exploitation of p53,as well as the wide-ranging response to p53 activation,makes it an attractive target for anti-cancer therapy.展开更多
Virus is a kind of microorganism and possesses simple structure and contains one nucleic acid,which must be replicated using the host cell system.It causes large-scale infectious diseases and poses serious threats to ...Virus is a kind of microorganism and possesses simple structure and contains one nucleic acid,which must be replicated using the host cell system.It causes large-scale infectious diseases and poses serious threats to the health,social well-being,and economic conditions of millions of people worldwide.Therefore,there is an urgent need to develop novel strategies for accurate diagnosis of virus infection to prevent disease transmission.Quantum dots(QDs)are typical fluorescence nanomaterials with high quantum yield,broad absorbance range,narrow and size-dependent emission,and good stability.QDs-based nanotechnology has been found to be effective method with rapid response,easy operation,high sensitivity,and good specificity,and has been widely applied for the detection of different viruses.However,until now,no systematic and critical review has been published on this important research area.Hence,in this review,we aim to provide a comprehensive coverage of various QDs-based virus detection methods.The fundamental investigations have been reviewed,including information related to the synthesis and biofunctionalization of QDs,QDs-based viral nucleic acid detection strategies,and QDs-based immunoassays.The challenges and perspectives regarding the potential application of QDs for virus detection is also discussed.展开更多
The organ and system-centered integrated curriculum has become a mainstream model of international medical education and an exploring model of Chinese medical education. However,there is no report on whether or how to...The organ and system-centered integrated curriculum has become a mainstream model of international medical education and an exploring model of Chinese medical education. However,there is no report on whether or how to integrate teaching contents of biological disciplines under this model in Chinese medical schools. Shanghai Jiao Tong University School of Medicine offers an integrated course named molecule,cell and tissue,which combines biochemistry,molecular biology,and cell biology with histology and physiology. This course not only reflects the integration of basic medicine and biology,but also focuses on molecular and cellular basis of structures and functions of human organs and systems. The course Molecule,Cell and Tissue breaks the boundaries of disciplines, provides integrated contents by deleting repeated contents,forms integrated key knowledge points,builds a reasonable integrated teaching team,modifies teaching methods,and adopts student-centered teaching models such as group report,co-teaching by teacher and students,and team-based learning. After 5 years of practice,this course has achieved satisfying teaching effect and can provide ideas and reference for the reform of Chinese basic medical education.展开更多
文摘Drugging the p53 pathway has been a goal for both academics and pharmaceutical companies since the designation of p53 as the'guardian of the genome'.Through growing understanding of p53 biology,we can see multiple routes for activation of both wild-type p53 function and restoration of mutant p53?In this review,we focus on small molecules that activate wild-type p53 and that do so in a nongenotoxit manner.In particular,we will describe potential approaches to targeting proteins that alter p53 stability and function through posttranslational modification,affect p53's subcellular localization,or target RNA synthesis or the synthesis of ribonucleotides.The plethora of pathways for exploitation of p53,as well as the wide-ranging response to p53 activation,makes it an attractive target for anti-cancer therapy.
基金supported by National Key Research and Development Program of China(2021YFA0910900)the National Natural Science Foundation of China(32222044,22104147)+5 种基金Shenzhen Municipal Science and Technology Innovation Council(RCYX20210609103823046)Youth Innovation Promotion Association CAS(2021359)Natural Science Foundation of Guangdong(2020A1515111130)Guangdong Provincial Key Laboratory of Synthetic Genomics(2019B030301006)Shenzhen Science and Technology Program(KQTD20180413181837372)Shenzhen Outstanding Talents Training Fund.
文摘Virus is a kind of microorganism and possesses simple structure and contains one nucleic acid,which must be replicated using the host cell system.It causes large-scale infectious diseases and poses serious threats to the health,social well-being,and economic conditions of millions of people worldwide.Therefore,there is an urgent need to develop novel strategies for accurate diagnosis of virus infection to prevent disease transmission.Quantum dots(QDs)are typical fluorescence nanomaterials with high quantum yield,broad absorbance range,narrow and size-dependent emission,and good stability.QDs-based nanotechnology has been found to be effective method with rapid response,easy operation,high sensitivity,and good specificity,and has been widely applied for the detection of different viruses.However,until now,no systematic and critical review has been published on this important research area.Hence,in this review,we aim to provide a comprehensive coverage of various QDs-based virus detection methods.The fundamental investigations have been reviewed,including information related to the synthesis and biofunctionalization of QDs,QDs-based viral nucleic acid detection strategies,and QDs-based immunoassays.The challenges and perspectives regarding the potential application of QDs for virus detection is also discussed.
基金financially supported by Shanghai Municipal Education Commission,Shanghai Key Course Construction Project(2015-37-59)
文摘The organ and system-centered integrated curriculum has become a mainstream model of international medical education and an exploring model of Chinese medical education. However,there is no report on whether or how to integrate teaching contents of biological disciplines under this model in Chinese medical schools. Shanghai Jiao Tong University School of Medicine offers an integrated course named molecule,cell and tissue,which combines biochemistry,molecular biology,and cell biology with histology and physiology. This course not only reflects the integration of basic medicine and biology,but also focuses on molecular and cellular basis of structures and functions of human organs and systems. The course Molecule,Cell and Tissue breaks the boundaries of disciplines, provides integrated contents by deleting repeated contents,forms integrated key knowledge points,builds a reasonable integrated teaching team,modifies teaching methods,and adopts student-centered teaching models such as group report,co-teaching by teacher and students,and team-based learning. After 5 years of practice,this course has achieved satisfying teaching effect and can provide ideas and reference for the reform of Chinese basic medical education.
