AFLP analysis was performed between a pair of thermo_sensitive genic male sterile (TGMS) rice allelic mutant lines (5460S and 5460F). The reaction conditions for rice AFLP assay were optimized. The relative efficienci...AFLP analysis was performed between a pair of thermo_sensitive genic male sterile (TGMS) rice allelic mutant lines (5460S and 5460F). The reaction conditions for rice AFLP assay were optimized. The relative efficiencies for polymorphism detection of RFLP, RAPD and AFLP were compared. The results indicated that the efficiency for polymorphism detection in rice was in the order of AFLP>RAPD>RFLP, and also indicated that AFLP was a powerful DNA molecular marker technique for polymorphism detection, especially in the case of extremely low polymorphism, such as isogenic lines and allelic mutant lines. Some of the AFLP products between the TGMS rice allelic mutant lines were cloned. Three of them were used as mixed probes to screen BAC library of rice line 5460S. 12 positive clones were screened out. In addition, the advantages and disadvantages of these three molecular marker systems were discussed.展开更多
分子对接技术属于计算机辅助药物设计(computer-aided drug design,CADD)的一种主要方法,近年来,在筛选中药药效物质、寻找药物作用于疾病的靶点以及探索中药的作用机制被广泛运用并取得了一定进展。本文通过文献综述,介绍了分子对接的...分子对接技术属于计算机辅助药物设计(computer-aided drug design,CADD)的一种主要方法,近年来,在筛选中药药效物质、寻找药物作用于疾病的靶点以及探索中药的作用机制被广泛运用并取得了一定进展。本文通过文献综述,介绍了分子对接的原理、分子对接机制以及部分常用的软件,并着重总结了分子对接技术在中药药效物质筛选及作用机制研究中的应用,此外对分子对接存在的问题进行讨论与展望,以期为中药临床研究及新药研发提供更多的理论依据。展开更多
目的前期研究及文献报道均表明野菊花黄酮类成分有明显抗炎活性,但其药效物质基础和作用机制尚未明确。该研究应用分子对接技术虚拟筛选野菊花黄酮抗炎活性的药效物质。方法搜集现已分离鉴定的34个野菊花黄酮类化合物组成配体数据库,选...目的前期研究及文献报道均表明野菊花黄酮类成分有明显抗炎活性,但其药效物质基础和作用机制尚未明确。该研究应用分子对接技术虚拟筛选野菊花黄酮抗炎活性的药效物质。方法搜集现已分离鉴定的34个野菊花黄酮类化合物组成配体数据库,选择IκB激酶β(IKK-β)、p38、环氧合酶2 (COX-2)、肿瘤坏死因子(TNF)等4个与抗炎活性密切相关的靶点组成受体数据库,应用Discovery Studio 3.0 (DS3.0)软件进行分子对接。结果通过分子对接虚拟筛选,筛选出打分总分高于阈值的黄酮类化合物共11个。结论对比分析了原配体与野菊花黄酮作用于各靶点的主要活性位点,初步推断了野菊花黄酮抗炎活性的作用机制,为研发抗炎制剂类药物提供了一定的参考。展开更多
The possible relationship between diabetes mellitus (DM) and colorectal cancer (CRC), concerning pathophysiological and molecular mechanisms is highlighted in this review. The most recent and complete articles and dev...The possible relationship between diabetes mellitus (DM) and colorectal cancer (CRC), concerning pathophysiological and molecular mechanisms is highlighted in this review. The most recent and complete articles and developments in this particular field were thoroughly reviewed. Common risk factors, such as obesity, sedentary lifestyle, and Western diet between DM and CRC, led to the theory that DM might be a causal agent for CRC development. Various studies have connected type 2 DM and CRC, either proximal or distal, in both sexes. Additionally, chronic insulin treatment has been linked with increased colorectal tumor risk among type 2 diabetic patients. Interestingly, elevated hemoglobin A1c has been proven to be an independent predictor of aggressive clinical behavior in CRC patients. These mechanisms include the insulin-like growth factor-hyperinsulinemia theory and the participation of oncogenic intracellular signaling pathways. Furthermore, it has been proposed that Cox-2 inhibitors might have a role in decreasing the incidence of CRC. Finally, the use of statins to reduce the risk for colon cancer in patients with diabetes has remained controversial. Diabetic patients over 50 should receive counseling regarding their elevated risk for CRC, and screening colonoscopy should be recommended before initiating insulin therapy. However, there are no current guidelines, and this strategy is not yet applicable to some countries, as the corresponding risk would not allow screening colonoscopy to be adopted. There is strong evidence to indicate that DM is a causal agent for CRC development. This conclusion provides new impetus for re-evaluating CRC screening worldwide.展开更多
文摘AFLP analysis was performed between a pair of thermo_sensitive genic male sterile (TGMS) rice allelic mutant lines (5460S and 5460F). The reaction conditions for rice AFLP assay were optimized. The relative efficiencies for polymorphism detection of RFLP, RAPD and AFLP were compared. The results indicated that the efficiency for polymorphism detection in rice was in the order of AFLP>RAPD>RFLP, and also indicated that AFLP was a powerful DNA molecular marker technique for polymorphism detection, especially in the case of extremely low polymorphism, such as isogenic lines and allelic mutant lines. Some of the AFLP products between the TGMS rice allelic mutant lines were cloned. Three of them were used as mixed probes to screen BAC library of rice line 5460S. 12 positive clones were screened out. In addition, the advantages and disadvantages of these three molecular marker systems were discussed.
文摘分子对接技术属于计算机辅助药物设计(computer-aided drug design,CADD)的一种主要方法,近年来,在筛选中药药效物质、寻找药物作用于疾病的靶点以及探索中药的作用机制被广泛运用并取得了一定进展。本文通过文献综述,介绍了分子对接的原理、分子对接机制以及部分常用的软件,并着重总结了分子对接技术在中药药效物质筛选及作用机制研究中的应用,此外对分子对接存在的问题进行讨论与展望,以期为中药临床研究及新药研发提供更多的理论依据。
文摘目的前期研究及文献报道均表明野菊花黄酮类成分有明显抗炎活性,但其药效物质基础和作用机制尚未明确。该研究应用分子对接技术虚拟筛选野菊花黄酮抗炎活性的药效物质。方法搜集现已分离鉴定的34个野菊花黄酮类化合物组成配体数据库,选择IκB激酶β(IKK-β)、p38、环氧合酶2 (COX-2)、肿瘤坏死因子(TNF)等4个与抗炎活性密切相关的靶点组成受体数据库,应用Discovery Studio 3.0 (DS3.0)软件进行分子对接。结果通过分子对接虚拟筛选,筛选出打分总分高于阈值的黄酮类化合物共11个。结论对比分析了原配体与野菊花黄酮作用于各靶点的主要活性位点,初步推断了野菊花黄酮抗炎活性的作用机制,为研发抗炎制剂类药物提供了一定的参考。
文摘The possible relationship between diabetes mellitus (DM) and colorectal cancer (CRC), concerning pathophysiological and molecular mechanisms is highlighted in this review. The most recent and complete articles and developments in this particular field were thoroughly reviewed. Common risk factors, such as obesity, sedentary lifestyle, and Western diet between DM and CRC, led to the theory that DM might be a causal agent for CRC development. Various studies have connected type 2 DM and CRC, either proximal or distal, in both sexes. Additionally, chronic insulin treatment has been linked with increased colorectal tumor risk among type 2 diabetic patients. Interestingly, elevated hemoglobin A1c has been proven to be an independent predictor of aggressive clinical behavior in CRC patients. These mechanisms include the insulin-like growth factor-hyperinsulinemia theory and the participation of oncogenic intracellular signaling pathways. Furthermore, it has been proposed that Cox-2 inhibitors might have a role in decreasing the incidence of CRC. Finally, the use of statins to reduce the risk for colon cancer in patients with diabetes has remained controversial. Diabetic patients over 50 should receive counseling regarding their elevated risk for CRC, and screening colonoscopy should be recommended before initiating insulin therapy. However, there are no current guidelines, and this strategy is not yet applicable to some countries, as the corresponding risk would not allow screening colonoscopy to be adopted. There is strong evidence to indicate that DM is a causal agent for CRC development. This conclusion provides new impetus for re-evaluating CRC screening worldwide.
