Objective： To observe the effects of water extract of Zuojin Pill （左金丸, ZJP） on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism. Methods： Effects of ZJP on SGC-7901 ...Objective： To observe the effects of water extract of Zuojin Pill （左金丸, ZJP） on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism. Methods： Effects of ZJP on SGC-7901 cells growth were determined by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide （MTT） assay, cell apoptosis and cell cycle were determined by flow cytornetry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 （Bcl-2）, Bcl-2 associated X protein （Bax） and cleaved caspase-3 and caspase-9. Results： ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation. Conclusions： ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.展开更多
基金Supported by the China Postdoctoral Science Foundation(No.2014M560666)
文摘Objective： To observe the effects of water extract of Zuojin Pill （左金丸, ZJP） on inhibiting the growth of human gastric cancer cell line SGC-7901 and its potential mechanism. Methods： Effects of ZJP on SGC-7901 cells growth were determined by 3-（4,5-dimethyl-2-thiazolyl）-2,5-diphenyl-2-H-tetrazolium bromide （MTT） assay, cell apoptosis and cell cycle were determined by flow cytornetry, and apoptosis induction was detected by means of DNA gel electrophoresis. The cellular mechanism of drug-induced cell death was unraveled by assaying oxidative injury level of SGC-7901 cell, mitochondrial membrane potentials, expression of apoptosis-related genes, such as B cell lymphoma/lewkmia-2 （Bcl-2）, Bcl-2 associated X protein （Bax） and cleaved caspase-3 and caspase-9. Results： ZJP exerted evident inhibitory effect on SGC-7901 cells by activating production of reactive oxygen species and elevating Bax/Bcl-2 ratio in SGC-7901 cells, leading to attenuation of mitochondrial membrane potential and DNA fragmentation. Conclusions： ZJP inhibits the cancer cell growth via activating mitochondria-dependent apoptosis pathway. ZJP can potentially serve as an antitumor agent.
