目的建立噁唑酮诱导的小鼠结肠炎模型并评价其在溃疡性结肠炎(UC)研究中的价值。方法BALB/c小鼠皮肤涂擦0.2 ml 3%噁唑酮致敏2次,5天后用0.15 ml 1%噁唑酮灌肠。监测小鼠疾病活动指数(DAI)、大体形态学评分、组织学损伤评分,并检测病变...目的建立噁唑酮诱导的小鼠结肠炎模型并评价其在溃疡性结肠炎(UC)研究中的价值。方法BALB/c小鼠皮肤涂擦0.2 ml 3%噁唑酮致敏2次,5天后用0.15 ml 1%噁唑酮灌肠。监测小鼠疾病活动指数(DAI)、大体形态学评分、组织学损伤评分,并检测病变结肠组织的髓过氧化物酶(MPO)活性、干扰素(IFN)-γ、白细胞介素(IL)-4含量。结果结肠炎模型小鼠的DAI、大体形态学评分、组织学损伤评分和MPO活性均较对照组明显增高,病变组织IL-4含量明显高于对照组,IFN-γ含量显著低于对照组。结论噁唑酮诱导的结肠炎属于2型辅助性T细胞(Th2)型炎症反应,类似于人类UC。噁唑酮诱导的小鼠结肠炎模型可作为UC发病机制研究和筛选有治疗潜力药物的重要工具。展开更多
AIM: To investigate the anti-inflammatory role of vasoactive intestinal peptide(VIP) in Aspergillus fumigatus(A. fumigatus) ketatitis.METHODS: Expression of VIP was tested by polymerase chain reaction(PCR) in ...AIM: To investigate the anti-inflammatory role of vasoactive intestinal peptide(VIP) in Aspergillus fumigatus(A. fumigatus) ketatitis.METHODS: Expression of VIP was tested by polymerase chain reaction(PCR) in C57BL/6 and BALB/c normal and A. fumigatus infected corneas. C57BL/6 mice were pretreated with recombinant(r) VIP, while BALB/c mice were pretreated with VIP antagonist, and then infected with A. fumigatus. Clinical score was recorded. Expression of pro-and anti-inflammatory cytokines, toll-like receptor 4(TLR4), lectin-like oxidized low-density lipoprotein receptor 1(LOX-1), and neutrophil infiltration were tested by PCR, enzyme-linked immunosorbent assay(ELISA), and myeloperoxidase(MPO) assay.RESULTS: VIP mR NA expression in BALB/c cornea was higher than C57BL/6 cornea at 1 and 3 d post infection(p.i.). rV IP treatment of C57BL/6 mice showed alleviated disease and down-regulated expression of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α), while IL-10 expression was up-regulated. Neutrophil infiltration and TLR4, IL-17 expression were decreased after rVIP treatment, while LOX-1 expression was up-regulated in C57BL/6. VIP antagonist pretreatment showed increased disease and higher IL-1β, TNF-α, TLR4, IL-17 and MPO levels, while IL-10 and LOX-1 levels were down-regulated in BALB/c mice.CONCLUSION: rVIP alleviate disease response of C57BL/6 mice. VIP antagonist resulted in worsened disease of BALB/c mice. VIP proposed anti-inflammatory role in A. fumigatus keratitis.展开更多
文摘目的建立噁唑酮诱导的小鼠结肠炎模型并评价其在溃疡性结肠炎(UC)研究中的价值。方法BALB/c小鼠皮肤涂擦0.2 ml 3%噁唑酮致敏2次,5天后用0.15 ml 1%噁唑酮灌肠。监测小鼠疾病活动指数(DAI)、大体形态学评分、组织学损伤评分,并检测病变结肠组织的髓过氧化物酶(MPO)活性、干扰素(IFN)-γ、白细胞介素(IL)-4含量。结果结肠炎模型小鼠的DAI、大体形态学评分、组织学损伤评分和MPO活性均较对照组明显增高,病变组织IL-4含量明显高于对照组,IFN-γ含量显著低于对照组。结论噁唑酮诱导的结肠炎属于2型辅助性T细胞(Th2)型炎症反应,类似于人类UC。噁唑酮诱导的小鼠结肠炎模型可作为UC发病机制研究和筛选有治疗潜力药物的重要工具。
基金Supported by the National Natural Science Foundation of China(No.81470609No.81500695)+1 种基金the Natural Science Foundation of Shandong Province(No.ZR2013HQ007No.ZR2017BH025)
文摘AIM: To investigate the anti-inflammatory role of vasoactive intestinal peptide(VIP) in Aspergillus fumigatus(A. fumigatus) ketatitis.METHODS: Expression of VIP was tested by polymerase chain reaction(PCR) in C57BL/6 and BALB/c normal and A. fumigatus infected corneas. C57BL/6 mice were pretreated with recombinant(r) VIP, while BALB/c mice were pretreated with VIP antagonist, and then infected with A. fumigatus. Clinical score was recorded. Expression of pro-and anti-inflammatory cytokines, toll-like receptor 4(TLR4), lectin-like oxidized low-density lipoprotein receptor 1(LOX-1), and neutrophil infiltration were tested by PCR, enzyme-linked immunosorbent assay(ELISA), and myeloperoxidase(MPO) assay.RESULTS: VIP mR NA expression in BALB/c cornea was higher than C57BL/6 cornea at 1 and 3 d post infection(p.i.). rV IP treatment of C57BL/6 mice showed alleviated disease and down-regulated expression of interleukin-1β(IL-1β) and tumor necrosis factor-α(TNF-α), while IL-10 expression was up-regulated. Neutrophil infiltration and TLR4, IL-17 expression were decreased after rVIP treatment, while LOX-1 expression was up-regulated in C57BL/6. VIP antagonist pretreatment showed increased disease and higher IL-1β, TNF-α, TLR4, IL-17 and MPO levels, while IL-10 and LOX-1 levels were down-regulated in BALB/c mice.CONCLUSION: rVIP alleviate disease response of C57BL/6 mice. VIP antagonist resulted in worsened disease of BALB/c mice. VIP proposed anti-inflammatory role in A. fumigatus keratitis.