Diclofenac(DIC)is often mixed into health products by unscrupulous merchants to claim antirheumatic effects.In this study,A highly sensitive group-selective monoclonal antibody(mAb)was prepared,and an immunochromatogr...Diclofenac(DIC)is often mixed into health products by unscrupulous merchants to claim antirheumatic effects.In this study,A highly sensitive group-selective monoclonal antibody(mAb)was prepared,and an immunochromatographic assay was developed for rapid screening of DIC,diclofenac methyl(DICA)and N-phenylanthranilic acid(PAA)in health teas and health beverages.In our quantitative analysis,the LOQs for the immunochromatographic test strip(ICS)for the detection of DIC(DICA and PAA)in health tea and health beverage were 0.17(1.13 and 1.34)and 0.12(0.25 and 0.53)ng/mL,respectively.This method was compared with LC-MS/MS method to detect the spiked samples,and the results were in good agreement with the LC-MS/MS,to verifying the effectiveness of the new method.Therefore,the ICS we have developed appears to be suitable for monitoring in-field residue levels of DIC,DICA and PAA in real samples.展开更多
Comprehensive Summary Talachalasins A-C(1-3),three new cytochalasans(CYTs),were isolated from deep-sea-derived fungus Talaromyces muroii sp.SCSIO 40439.The structures of 1-3,including absolute configurations,were dete...Comprehensive Summary Talachalasins A-C(1-3),three new cytochalasans(CYTs),were isolated from deep-sea-derived fungus Talaromyces muroii sp.SCSIO 40439.The structures of 1-3,including absolute configurations,were determined based on HRESIMS,NMR spectroscopic data,ECD comparison,and single crystal X-ray diffraction analysis.Talachalasins A-C(1-3)represented the first examples of cytochalasans possessing a unique 16β-methyl group,distinct from>500 reported CYTs.Talachalasin C(3)was an unprecedented 20,21-seco-cytochalasans characterized by a 2-oxabicyclo[3.3.1]nonan-3-one unit.Talachalasin A(1)displayed moderate cytotoxic activity against four tumor cell lines(IC_(50)3.40-10.02μM).Talachalasin B(2)showed comparable anti-RSV and HSV-1 activities(IC5012.5μM or IC5020.0μM).展开更多
基金financially supported by National Key R&D Program(2019YFC1604602)Suzhou Science and Technology Committee Program(SNG2020056).
文摘Diclofenac(DIC)is often mixed into health products by unscrupulous merchants to claim antirheumatic effects.In this study,A highly sensitive group-selective monoclonal antibody(mAb)was prepared,and an immunochromatographic assay was developed for rapid screening of DIC,diclofenac methyl(DICA)and N-phenylanthranilic acid(PAA)in health teas and health beverages.In our quantitative analysis,the LOQs for the immunochromatographic test strip(ICS)for the detection of DIC(DICA and PAA)in health tea and health beverage were 0.17(1.13 and 1.34)and 0.12(0.25 and 0.53)ng/mL,respectively.This method was compared with LC-MS/MS method to detect the spiked samples,and the results were in good agreement with the LC-MS/MS,to verifying the effectiveness of the new method.Therefore,the ICS we have developed appears to be suitable for monitoring in-field residue levels of DIC,DICA and PAA in real samples.
基金supported in part by the National Natural Science Technology Project of Hainan Province(ZDKJ202018)K.C.Wong Education Foundation(GJTD-2020-12)+1 种基金the Guangdong Provincial Special Fund for Marine Economic Development Project(GDNRC[2021]48)Southern Marine Science and Engineering Guangdong Laboratory(Guangzhou)(GML2019ZD0406).
文摘Comprehensive Summary Talachalasins A-C(1-3),three new cytochalasans(CYTs),were isolated from deep-sea-derived fungus Talaromyces muroii sp.SCSIO 40439.The structures of 1-3,including absolute configurations,were determined based on HRESIMS,NMR spectroscopic data,ECD comparison,and single crystal X-ray diffraction analysis.Talachalasins A-C(1-3)represented the first examples of cytochalasans possessing a unique 16β-methyl group,distinct from>500 reported CYTs.Talachalasin C(3)was an unprecedented 20,21-seco-cytochalasans characterized by a 2-oxabicyclo[3.3.1]nonan-3-one unit.Talachalasin A(1)displayed moderate cytotoxic activity against four tumor cell lines(IC_(50)3.40-10.02μM).Talachalasin B(2)showed comparable anti-RSV and HSV-1 activities(IC5012.5μM or IC5020.0μM).
