目的:验证耳穴磁贴在妇科开腹手术辅助麻醉的镇痛镇静效应和术后对机体功能恢复的作用。方法:将妇科开腹手术患者92例随机分为3组,耳穴组(31例)于手术前夜在双侧神门、皮质下、子宫及盆腔等相关耳穴区贴敷磁珠并按压;安慰组(31例)在所...目的:验证耳穴磁贴在妇科开腹手术辅助麻醉的镇痛镇静效应和术后对机体功能恢复的作用。方法:将妇科开腹手术患者92例随机分为3组,耳穴组(31例)于手术前夜在双侧神门、皮质下、子宫及盆腔等相关耳穴区贴敷磁珠并按压;安慰组(31例)在所选耳穴区贴敷同样外观的无磁珠胶布;空白组(30例)不予任何干预措施。观察术前夜至术后3天的精神、胃肠功能等变化。结果:与安慰组、空白组比较,耳穴组的术后焦虑自评量表(SAS)评分较少(25.5±0.81 vs 28.9±3.19、28.3+2.36,均P<0.01),加用氟芬合剂的剂量较少[(2.5±1.1)mL vs(3.4±1.8)mL、(3.2±1.6)mL,均P<0.05),术后排气时间较早[(34.2±12.1)h vs(46.3±10.9)h、(43.2±14.8)h,均P<0.01],术前及术毕血清β-内啡肽水平较高(均P<0.05)。结论:耳穴磁贴对妇科开腹手术具有一定的稳定情绪、镇痛及调整机体功能的作用。展开更多
Background：We investigated possible biomarkers for endometriosis （EM） using the ClinProt technique and proteomics methods.Methods：We enrolled 50 patients with EM,34 with benign ovarian neoplasms and 40 healthy vol...Background：We investigated possible biomarkers for endometriosis （EM） using the ClinProt technique and proteomics methods.Methods：We enrolled 50 patients with EM,34 with benign ovarian neoplasms and 40 healthy volunteers in this study.Serum proteomic spectra were generated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MS） combined with weak cationic exchange （WCX） magnetic beads.Possible biomarkers were analyzed by a random and repeat pattern model-validation method that we designed,and ClinProtools software,results were refined using online liquid chromatography-tandem MS.Results：We found a cluster of 5 peptides （4210,5264,2660,5635,and 5904 Da）,using 3 peptides （4210,5904,2660 Da） to discriminate EM patients from healthy volunteers,with 96.67％ sensitivity and 100％ specificity.We selected 4210 and 5904 m/z,which differed most between patients with EM and controls,and identified them as fragments of ATP1B4,and the fibrinogen alpha （FGA） isoform 1/2 of the FGA chain precursor,respectively.Conclusions：ClinProt can identify EM biomarkers,which-most notably-distinguish even early-stage or minimal disease.We found 5 stable peaks at 4210,5264,2660,5635,and 5904 Da as potential EM biomarkers,the strongest of which were associated with ATP1B4 （4210 Da） and FGA （5904 Da）; this indicates that ATP1B4 and FGA are associated with EM pathogenesis.展开更多
A microsatellite-enriched library of plateau pika （Ochotona curzoniae） was constructed according to the strong affinity between biotin and streptavidin. Firstly, genomic DNA was fragmented by ultrasonication, which ...A microsatellite-enriched library of plateau pika （Ochotona curzoniae） was constructed according to the strong affinity between biotin and streptavidin. Firstly, genomic DNA was fragmented by ultrasonication, which is a major improvement over traditional methods. Linker-ligated DNA fragments were hybridized with biotinylated microsatellite probes, and then were subjected to streptavidin-coated magnetic beads. PCR amplification was performed to obtain double-stranded DNA fragments containing microsatellites. Ligation and transformation were carried out by using the pGEM-T Vector System I and Escherichia coli DH10B competent cells. Sequencing results showed that 80.2% of clones contained microsatellite repeat motif. Several modifications make this protocol time-efficient and technically easier than the traditional ones; particularly, composition and relative abundance of microsatellite repeats in plateau pika genome were truly represented through the optimized PCR conditions. This method has also been successfully applied to construct microsatellite-enriched genomic libraries of Chinese hamster （Cricetulus griseus） and small abalone [Haliotis diversicolor （Reeve）] with high rates of positive clones, demonstrating its feasibility and stability.展开更多
黄曲霉毒素B_1(aflatoxin B_1,AFB_1)适配体共价偶联于磁珠表面制备适配体亲和磁珠(aptamer affinity magnetic beads,AAMB),将AAMB、孵育液、洗涤液和洗脱液预封装于试剂条中开发了AFB_1单样本前处理试剂盒,借助真菌毒素全自动净化仪...黄曲霉毒素B_1(aflatoxin B_1,AFB_1)适配体共价偶联于磁珠表面制备适配体亲和磁珠(aptamer affinity magnetic beads,AAMB),将AAMB、孵育液、洗涤液和洗脱液预封装于试剂条中开发了AFB_1单样本前处理试剂盒,借助真菌毒素全自动净化仪实现了AFB_1前处理的自动化,使用超高效液相色谱仪对洗脱液中AFB_1的含量进行测定。通过优化磁珠粒径、适配体间接臂长度、适配体亲和磁珠用量、孵育液体积确定了AAMB的制备条件以及AFB_1单样本前处理试剂盒的使用条件,对比了手动和自动两种前处理方式下AAMB回收率的差异。在优化条件下,AFB_1的检出限(limit of detection,LOD)为0.6 ng/mL,玉米和小麦中低、中、高三个水平的加标回收率在92.8%~110.1%之间,日内精密度的相对标准偏差(relative standard deviation,RSD)小于5.1%,日间精密度的RSD为6.7%,具有良好的灵敏度、准确性和重复性;粮油基质(玉米、糙米、花生油和花生酱)中AFB_1成分国家有证标准物质的测定结果在其扩展范围内,具有良好的基质适用性。开发的单样本前处理试剂盒借助真菌毒素全自动净化仪仅需一步操作即可在20 min内自动完成10个样品的前处理,具有时间短、通量高、批次间差异小的优点,在真菌毒素定量检测方面具有良好的应用前景,为开发针对其它靶标的自动化前处理方法奠定了基础。展开更多
文摘目的:验证耳穴磁贴在妇科开腹手术辅助麻醉的镇痛镇静效应和术后对机体功能恢复的作用。方法:将妇科开腹手术患者92例随机分为3组,耳穴组(31例)于手术前夜在双侧神门、皮质下、子宫及盆腔等相关耳穴区贴敷磁珠并按压;安慰组(31例)在所选耳穴区贴敷同样外观的无磁珠胶布;空白组(30例)不予任何干预措施。观察术前夜至术后3天的精神、胃肠功能等变化。结果:与安慰组、空白组比较,耳穴组的术后焦虑自评量表(SAS)评分较少(25.5±0.81 vs 28.9±3.19、28.3+2.36,均P<0.01),加用氟芬合剂的剂量较少[(2.5±1.1)mL vs(3.4±1.8)mL、(3.2±1.6)mL,均P<0.05),术后排气时间较早[(34.2±12.1)h vs(46.3±10.9)h、(43.2±14.8)h,均P<0.01],术前及术毕血清β-内啡肽水平较高(均P<0.05)。结论:耳穴磁贴对妇科开腹手术具有一定的稳定情绪、镇痛及调整机体功能的作用。
基金grants from the National Natural Science Foundation of China，supported by Peking University People＇s Hospital Research and Development Funds
文摘Background：We investigated possible biomarkers for endometriosis （EM） using the ClinProt technique and proteomics methods.Methods：We enrolled 50 patients with EM,34 with benign ovarian neoplasms and 40 healthy volunteers in this study.Serum proteomic spectra were generated by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry （MS） combined with weak cationic exchange （WCX） magnetic beads.Possible biomarkers were analyzed by a random and repeat pattern model-validation method that we designed,and ClinProtools software,results were refined using online liquid chromatography-tandem MS.Results：We found a cluster of 5 peptides （4210,5264,2660,5635,and 5904 Da）,using 3 peptides （4210,5904,2660 Da） to discriminate EM patients from healthy volunteers,with 96.67％ sensitivity and 100％ specificity.We selected 4210 and 5904 m/z,which differed most between patients with EM and controls,and identified them as fragments of ATP1B4,and the fibrinogen alpha （FGA） isoform 1/2 of the FGA chain precursor,respectively.Conclusions：ClinProt can identify EM biomarkers,which-most notably-distinguish even early-stage or minimal disease.We found 5 stable peaks at 4210,5264,2660,5635,and 5904 Da as potential EM biomarkers,the strongest of which were associated with ATP1B4 （4210 Da） and FGA （5904 Da）; this indicates that ATP1B4 and FGA are associated with EM pathogenesis.
文摘A microsatellite-enriched library of plateau pika （Ochotona curzoniae） was constructed according to the strong affinity between biotin and streptavidin. Firstly, genomic DNA was fragmented by ultrasonication, which is a major improvement over traditional methods. Linker-ligated DNA fragments were hybridized with biotinylated microsatellite probes, and then were subjected to streptavidin-coated magnetic beads. PCR amplification was performed to obtain double-stranded DNA fragments containing microsatellites. Ligation and transformation were carried out by using the pGEM-T Vector System I and Escherichia coli DH10B competent cells. Sequencing results showed that 80.2% of clones contained microsatellite repeat motif. Several modifications make this protocol time-efficient and technically easier than the traditional ones; particularly, composition and relative abundance of microsatellite repeats in plateau pika genome were truly represented through the optimized PCR conditions. This method has also been successfully applied to construct microsatellite-enriched genomic libraries of Chinese hamster （Cricetulus griseus） and small abalone [Haliotis diversicolor （Reeve）] with high rates of positive clones, demonstrating its feasibility and stability.
文摘黄曲霉毒素B_1(aflatoxin B_1,AFB_1)适配体共价偶联于磁珠表面制备适配体亲和磁珠(aptamer affinity magnetic beads,AAMB),将AAMB、孵育液、洗涤液和洗脱液预封装于试剂条中开发了AFB_1单样本前处理试剂盒,借助真菌毒素全自动净化仪实现了AFB_1前处理的自动化,使用超高效液相色谱仪对洗脱液中AFB_1的含量进行测定。通过优化磁珠粒径、适配体间接臂长度、适配体亲和磁珠用量、孵育液体积确定了AAMB的制备条件以及AFB_1单样本前处理试剂盒的使用条件,对比了手动和自动两种前处理方式下AAMB回收率的差异。在优化条件下,AFB_1的检出限(limit of detection,LOD)为0.6 ng/mL,玉米和小麦中低、中、高三个水平的加标回收率在92.8%~110.1%之间,日内精密度的相对标准偏差(relative standard deviation,RSD)小于5.1%,日间精密度的RSD为6.7%,具有良好的灵敏度、准确性和重复性;粮油基质(玉米、糙米、花生油和花生酱)中AFB_1成分国家有证标准物质的测定结果在其扩展范围内,具有良好的基质适用性。开发的单样本前处理试剂盒借助真菌毒素全自动净化仪仅需一步操作即可在20 min内自动完成10个样品的前处理,具有时间短、通量高、批次间差异小的优点,在真菌毒素定量检测方面具有良好的应用前景,为开发针对其它靶标的自动化前处理方法奠定了基础。
