【目的】检测非小细胞肺癌(NSCLC)患者血清及肺癌组织中巨噬细胞移动抑制因子(MIF)的表达水平,探讨MIF在NSCLC患者中的表达及其临床意义。【方法】NSCLC组患者66例,肺部良性病变组患者22例,健康体检者30例,用酶联免疫吸附法(ELISA)检测...【目的】检测非小细胞肺癌(NSCLC)患者血清及肺癌组织中巨噬细胞移动抑制因子(MIF)的表达水平,探讨MIF在NSCLC患者中的表达及其临床意义。【方法】NSCLC组患者66例,肺部良性病变组患者22例,健康体检者30例,用酶联免疫吸附法(ELISA)检测各组血清中MIF的浓度水平;用免疫组织化学法(IHC)检测两组患者肺组织中MIF的表达水平。应用SPSS17.0统计学软件进行分析。【结果】(1)NSCLC患者血清MIF浓度显著高于健康人(14.79 ng/mL vs 10.69 ng/mL,P=0.001),略高于肺良性病变组但无统计学差异(14.79 ng/mL vs 13.68 ng/mL,P=0.580)。NSCLC患者血清中MIF的水平与肿瘤的分化程度、临床分期及患者的性别、吸烟状态有关。(2)免疫组化结果显示,肺癌组织中MIF的高表达率显著高于肺良性病变组织(30.3%vs 4.5%,P=0.014),且MIF的表达程度与肿瘤的病理类型、临床分期相关。(3)相关性分析显示,NSCLC患者血清MIF水平与肺癌组织的MIF高表达率呈正相关(P<0.05)。【结论】MIF在NSCLC患者中的表达与肺癌的临床病理特征有一定的关系,对NSCLC的诊断及临床评估有一定的价值。展开更多
Mammalian macrophage migration inhibitory factor （MIF） plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homo...Mammalian macrophage migration inhibitory factor （MIF） plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homologue in fish septicemia. The authors have cloned the MIF homologue in large yellow croaker Pseudosciaena crocea （LycMIF） using RACE approach. The full-length cDNA of LycMIF was 634 bases and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. As demonstrated by RT-PCR and QRT-PCR assay, MIF mRNAs were constitutively expressed in 11 selected tissues and were abundant in brain and liver. Moreover, the LycMIF transcripts in the liver and head kidney were responsive to bacteria infection and could be significantly up-regulated. Our results provide the first direct evidence that fish MIF was implicated in pathogenesis of fish vibrosis and play an important role in response to bacteria infection.展开更多
文摘该研究旨在了解小檗碱对急性缺血性脑卒中(acute cerebral ischemic stroke,AIS)患者颈动脉粥样硬化斑块及血清炎症因子的作用。将120例AIS患者按照随机数字表分为小檗碱组(n=60)和常规组(n=60)。常规组按照最新AIS诊治指南给予常规治疗,小檗碱组在常规组治疗基础上加用盐酸小檗碱片(300 mg/次,每日3次)。测定所有患者治疗前后炎症因子浓度、神经功能缺损评分及颈动脉粥样硬化斑块指标[包括颈动脉粥样硬化斑块总面积(total plaque area,TPA)、内膜中层厚度(intima-media thickness,IMT)及不稳定性斑块数目]的变化并进行比较。研究结果发现,第1天,2组患者炎症因子浓度,NIHSS(national institute of health stroke scale)评分及颈动脉粥样硬化斑块指标的比较无明显统计学差异且炎症因子水平与NIHSS评分呈正相关(P<0.05)。第14天较第1天,2组患者炎症因子浓度及NIHSS评分显著下降(P<0.05)。第14天,小檗碱组较常规组炎症因子浓度及NIHSS评分下降明显(P<0.05)。第90天较第1天,2组患者TPA及不稳定性斑块数目显著减低,差异有统计学意义(P<0.05),而IMT有下降趋势,差异无统计学意义。第90天,小檗碱组较常规组TPA及不稳定性斑块数目减少明显(P<0.05),mRS(modified rankin scale)评分显著偏低且短期预后良好率显著偏高(P<0.05)。2组不良反应发生率无明显差异。该研究结果表明在常规治疗基础上加用小檗碱可显著降低AIS患者急性期血清炎症因子水平,并在一定程度上减轻AIS患者的颈动脉粥样硬化程度,促进神经功能的恢复,改善缺血性卒中病人的短期预后。
文摘【目的】检测非小细胞肺癌(NSCLC)患者血清及肺癌组织中巨噬细胞移动抑制因子(MIF)的表达水平,探讨MIF在NSCLC患者中的表达及其临床意义。【方法】NSCLC组患者66例,肺部良性病变组患者22例,健康体检者30例,用酶联免疫吸附法(ELISA)检测各组血清中MIF的浓度水平;用免疫组织化学法(IHC)检测两组患者肺组织中MIF的表达水平。应用SPSS17.0统计学软件进行分析。【结果】(1)NSCLC患者血清MIF浓度显著高于健康人(14.79 ng/mL vs 10.69 ng/mL,P=0.001),略高于肺良性病变组但无统计学差异(14.79 ng/mL vs 13.68 ng/mL,P=0.580)。NSCLC患者血清中MIF的水平与肿瘤的分化程度、临床分期及患者的性别、吸烟状态有关。(2)免疫组化结果显示,肺癌组织中MIF的高表达率显著高于肺良性病变组织(30.3%vs 4.5%,P=0.014),且MIF的表达程度与肿瘤的病理类型、临床分期相关。(3)相关性分析显示,NSCLC患者血清MIF水平与肺癌组织的MIF高表达率呈正相关(P<0.05)。【结论】MIF在NSCLC患者中的表达与肺癌的临床病理特征有一定的关系,对NSCLC的诊断及临床评估有一定的价值。
基金The National Natural Science Foundation of China under contract No. 40976096the National Department Public Benefit Research Foundation of China under contract No. 200903029the National High Technology Research and Development Program of China (863 Program) under contract No. 2006AA10A405
文摘Mammalian macrophage migration inhibitory factor （MIF） plays an important role as an indispensable mediator in the pathogenesis of inflammatory disease like septicemia, but little is known about the role of MIF homologue in fish septicemia. The authors have cloned the MIF homologue in large yellow croaker Pseudosciaena crocea （LycMIF） using RACE approach. The full-length cDNA of LycMIF was 634 bases and contained an ORF of 345 bases encoding a protein of 115 amino acid residues. As demonstrated by RT-PCR and QRT-PCR assay, MIF mRNAs were constitutively expressed in 11 selected tissues and were abundant in brain and liver. Moreover, the LycMIF transcripts in the liver and head kidney were responsive to bacteria infection and could be significantly up-regulated. Our results provide the first direct evidence that fish MIF was implicated in pathogenesis of fish vibrosis and play an important role in response to bacteria infection.
