Small heat shock proteins (sHSPs) exist ubiquitously among all organisms, with a variety of functions. All small heat shock proteins assemble into a native large oligomeric state containing 9–40 monomers. The sHSPs s...Small heat shock proteins (sHSPs) exist ubiquitously among all organisms, with a variety of functions. All small heat shock proteins assemble into a native large oligomeric state containing 9–40 monomers. The sHSPs show chaperone-like activity to prevent the aggregation of nonnative proteins under stressful cellular conditions such as non-optimal temperatures, pH changes, osmotic pressure, and exposure to toxic chemicals. It was found that a common dimeric subunit of sHSPs might be the major active species, but whether the native large oligomeric state is only a storage state or a state crucial to its molecular chaperone activity is still under debate. The native large oligomeric state of the small heat shock protein from a hyperthermophilic methanarchaeon, Methanococcus jannaschii (Mj HSP 16.5), is a stable icositetramer, which is a symmetric hollow sphere that is very stable even at 85°C, and no small active subunit has been detected till now. Our results show that Mj sHSP 16.5 changes into small and active oligomeric state at pH 3, likely as octamers (average result) at 25°C, and dimers at 65°C. The dimer of Mj HSP 16.5 at pH 3.0 and 65°C is very active and efficient, even 7-fold more efficient than the high-temperature-activated icositetramer at neutral pH. Monomer exchange can be observed between dimers of Mj HSP 16.5 at pH 3.0 and 65°C. These results not only demonstrate that the icositetramer structure of Mj sHSP16.5 is not necessary for its molecular chaperone activity, but also suggest that Mj sHSP16.5 is a very efficient chaperone acting at high temperature and under the acidic condition. Even though it is not clear whether the native environment of Methanococcus jannaschii is acidic or not, given its ability to excrete acidic compounds, it is likely that Methanococcus jannaschii will encounter acidic internal or external environments at high temperature. Our results demonstrate that Mj HSP 16.5 may help Methanococcus jannaschii to survive better under those extreme environmental conditions.展开更多
A high pulse repetition frequency（PRF）, high energy Ho：YAG laser directly pumped by a Tm-doped fiber laser and its application to a mid-infrared ZnGeP_2（ZGP） optical parametric oscillator（OPO） is demonstrated.T...A high pulse repetition frequency（PRF）, high energy Ho：YAG laser directly pumped by a Tm-doped fiber laser and its application to a mid-infrared ZnGeP_2（ZGP） optical parametric oscillator（OPO） is demonstrated.The maximum polarized 2.09 μm laser pulse energy is 13.46 mJ at a PRF of 1 k Hz. The corresponding peak power reaches 504 kW. In a double-resonant ZGP-OPO, a maximum mid-infrared laser pulse energy of 1.25 m J,corresponding to a peak power of 79 kW, is accomplished at a PRF of 3 kHz. The nonlinear conversion efficiency reaches 41.7%. The nonlinear slope efficiency reaches 53.3%.展开更多
This paper presents a performance study of silicon-based multi-junction (MJ) solar cell which is produced by Spectrolab is the most efficient solar cell in the world, with a record efficiency of over 40%. The I-V char...This paper presents a performance study of silicon-based multi-junction (MJ) solar cell which is produced by Spectrolab is the most efficient solar cell in the world, with a record efficiency of over 40%. The I-V characteristics of solar cells were investigated for different environmental conditions (both at indoor and outdoor). Photovoltaic (PV) parameters like short circuit current (ISC), open circuit voltage (VOC), fill factor (FF) and efficiency (?) were evaluated for the fabricated cell. The fabricated cell provides I-V characteristics curve which shows that the maximum efficiency of the cell is obtained when the cell temperature is lower. It was also observed that, efficiencies of the cells are about 17% to 24% at indoor and 7% to 20% at outdoor and fill factors are 0.40 to 0.71. From the result, it can also be found that fill factor is directly affected by the values of the cells series and shunt resistance.展开更多
We demonstrate eftlcient generation of continuous spectrum centered at 40Ohm from solid thin plates. By frequency doubling of 0.8m J, 3Ors Ti：sapphire laser pulses with a BBO crystal, 0.2m J, 33fs laser pulses at 400...We demonstrate eftlcient generation of continuous spectrum centered at 40Ohm from solid thin plates. By frequency doubling of 0.8m J, 3Ors Ti：sapphire laser pulses with a BBO crystal, 0.2m J, 33fs laser pulses at 400nm are generated. Focusing the 400-nm pulses into 7 thin fused silica plates, we obtain 0.15mJ continuous spectrum covering 350-450 nm. After compressing by 3 pairs of chirped mirrors, 0. 12 m J, 8.6 fs pulses are achieved. To the best of our knowledge, this is the first time that sub-10-fs pulses centered at 400nm are generated by solid thin plates, which shows that spectral broadening in solid-state materials works not only at 800nm but also at different wavelengths.展开更多
A hybrid-pumped Nd：YAG pulse laser with a double-pass two-rod configuration is presented. The focal length of offset lens is particularly studied to compensate for the thermal lens effect and depolarization. For inpu...A hybrid-pumped Nd：YAG pulse laser with a double-pass two-rod configuration is presented. The focal length of offset lens is particularly studied to compensate for the thermal lens effect and depolarization. For input pulse energy of 141 μJ with pulse duration of 754ps, the pulse laser system delivers 526mJ pulse energy and 728ps pulse width output at lO Hz with pulse profile shape preservation. The energy stability of the laser pulse is less than 3%, and the beam quality factor M2 is less than 2.26.展开更多
The question was on the mind of every NBA fan last January: Whocould possibly be the next Michael Jordan? Turns out the answer wasn t thenew MJ after all. It was the old Tim Duncan. That s old as in... "old schoo...The question was on the mind of every NBA fan last January: Whocould possibly be the next Michael Jordan? Turns out the answer wasn t thenew MJ after all. It was the old Tim Duncan. That s old as in... "old school." Duncan s game is built on solidfundamentals, hard work and team play. Just as Doctor Naismith intended. Of course, so was Jordan s game. MJ just preferred to play above therim for the first half-dozen years of his career. before he figured out the展开更多
基金Supported by the National Natural Science Foundation of China (Grant Nos. 20203001, 20673003, and 30490245)Ministry of Science and Technology of China (Grant No. 2006AA02Z301)
文摘Small heat shock proteins (sHSPs) exist ubiquitously among all organisms, with a variety of functions. All small heat shock proteins assemble into a native large oligomeric state containing 9–40 monomers. The sHSPs show chaperone-like activity to prevent the aggregation of nonnative proteins under stressful cellular conditions such as non-optimal temperatures, pH changes, osmotic pressure, and exposure to toxic chemicals. It was found that a common dimeric subunit of sHSPs might be the major active species, but whether the native large oligomeric state is only a storage state or a state crucial to its molecular chaperone activity is still under debate. The native large oligomeric state of the small heat shock protein from a hyperthermophilic methanarchaeon, Methanococcus jannaschii (Mj HSP 16.5), is a stable icositetramer, which is a symmetric hollow sphere that is very stable even at 85°C, and no small active subunit has been detected till now. Our results show that Mj sHSP 16.5 changes into small and active oligomeric state at pH 3, likely as octamers (average result) at 25°C, and dimers at 65°C. The dimer of Mj HSP 16.5 at pH 3.0 and 65°C is very active and efficient, even 7-fold more efficient than the high-temperature-activated icositetramer at neutral pH. Monomer exchange can be observed between dimers of Mj HSP 16.5 at pH 3.0 and 65°C. These results not only demonstrate that the icositetramer structure of Mj sHSP16.5 is not necessary for its molecular chaperone activity, but also suggest that Mj sHSP16.5 is a very efficient chaperone acting at high temperature and under the acidic condition. Even though it is not clear whether the native environment of Methanococcus jannaschii is acidic or not, given its ability to excrete acidic compounds, it is likely that Methanococcus jannaschii will encounter acidic internal or external environments at high temperature. Our results demonstrate that Mj HSP 16.5 may help Methanococcus jannaschii to survive better under those extreme environmental conditions.
基金supported by the National Natural Science Foundation of China(No.61275146)the National Key Research and Development Program of China(No.2016YFB0402104)
文摘A high pulse repetition frequency（PRF）, high energy Ho：YAG laser directly pumped by a Tm-doped fiber laser and its application to a mid-infrared ZnGeP_2（ZGP） optical parametric oscillator（OPO） is demonstrated.The maximum polarized 2.09 μm laser pulse energy is 13.46 mJ at a PRF of 1 k Hz. The corresponding peak power reaches 504 kW. In a double-resonant ZGP-OPO, a maximum mid-infrared laser pulse energy of 1.25 m J,corresponding to a peak power of 79 kW, is accomplished at a PRF of 3 kHz. The nonlinear conversion efficiency reaches 41.7%. The nonlinear slope efficiency reaches 53.3%.
文摘This paper presents a performance study of silicon-based multi-junction (MJ) solar cell which is produced by Spectrolab is the most efficient solar cell in the world, with a record efficiency of over 40%. The I-V characteristics of solar cells were investigated for different environmental conditions (both at indoor and outdoor). Photovoltaic (PV) parameters like short circuit current (ISC), open circuit voltage (VOC), fill factor (FF) and efficiency (?) were evaluated for the fabricated cell. The fabricated cell provides I-V characteristics curve which shows that the maximum efficiency of the cell is obtained when the cell temperature is lower. It was also observed that, efficiencies of the cells are about 17% to 24% at indoor and 7% to 20% at outdoor and fill factors are 0.40 to 0.71. From the result, it can also be found that fill factor is directly affected by the values of the cells series and shunt resistance.
基金Supported by the National Natural Science Foundation of China under Grant Nos 11434016,11574384 and 11674386the National Key Basic Research Program of China under Grant Nos 2013CB922401 and 2013CB922402
文摘We demonstrate eftlcient generation of continuous spectrum centered at 40Ohm from solid thin plates. By frequency doubling of 0.8m J, 3Ors Ti：sapphire laser pulses with a BBO crystal, 0.2m J, 33fs laser pulses at 400nm are generated. Focusing the 400-nm pulses into 7 thin fused silica plates, we obtain 0.15mJ continuous spectrum covering 350-450 nm. After compressing by 3 pairs of chirped mirrors, 0. 12 m J, 8.6 fs pulses are achieved. To the best of our knowledge, this is the first time that sub-10-fs pulses centered at 400nm are generated by solid thin plates, which shows that spectral broadening in solid-state materials works not only at 800nm but also at different wavelengths.
文摘A hybrid-pumped Nd：YAG pulse laser with a double-pass two-rod configuration is presented. The focal length of offset lens is particularly studied to compensate for the thermal lens effect and depolarization. For input pulse energy of 141 μJ with pulse duration of 754ps, the pulse laser system delivers 526mJ pulse energy and 728ps pulse width output at lO Hz with pulse profile shape preservation. The energy stability of the laser pulse is less than 3%, and the beam quality factor M2 is less than 2.26.
文摘The question was on the mind of every NBA fan last January: Whocould possibly be the next Michael Jordan? Turns out the answer wasn t thenew MJ after all. It was the old Tim Duncan. That s old as in... "old school." Duncan s game is built on solidfundamentals, hard work and team play. Just as Doctor Naismith intended. Of course, so was Jordan s game. MJ just preferred to play above therim for the first half-dozen years of his career. before he figured out the
