Rice tillering is one of the most important agronomic traits that determine grain yields.Our previous study has demonstrated that the MONOCULM1 （MOC1） gene is a key component that controls the formation of rice till...Rice tillering is one of the most important agronomic traits that determine grain yields.Our previous study has demonstrated that the MONOCULM1 （MOC1） gene is a key component that controls the formation of rice tiller buds.To further elucidate the molecular mechanism of MOC1 involved in the regulation of rice tillering,we performed a yeast-two-hybrid screening to identify MOC1 interacting proteins （MIPs）.Here we reported that MIP1 interacted with MOC1 both in vitro and in vivo.The overexpression of MIP1 resulted in enhanced tillering and reduced plant height.In-depth characterization of the context of MIP1 and MOC1 would further our understanding of molecular regulatory mechanisms of rice tillering.展开更多
目的探讨调节正常T细胞表达和分泌活性因子(Regulated on activation normal T cell expressed and secre-ted,RANTES),巨噬细胞炎性蛋白(Macrophage inflammatory protein-1,MIP-1α)及趋化因子受体CCR5在特应性皮炎(AD)发病中的作用...目的探讨调节正常T细胞表达和分泌活性因子(Regulated on activation normal T cell expressed and secre-ted,RANTES),巨噬细胞炎性蛋白(Macrophage inflammatory protein-1,MIP-1α)及趋化因子受体CCR5在特应性皮炎(AD)发病中的作用。方法采集30例AD患者及10例健康对照者血液,分离血清和外周血单个核细胞(PBMC),双抗体夹心ELISA法检测PBMC产生的RANTES、MIP-1α含量,荧光定量PCR检测外周血PBMC表达的CCR5 mRNA水平。结果特应性皮炎患者与健康对照者血清RANTES含量分别为(55.7±3.4)μg/L和(35.6±1.8)μg/L,差异有显著性(t=3.036,P<0.01),且RANTES水平与SCORAD呈正相关(r=0.889,P<0.05);MIP-1α含量分别为(51.8±3.6)μg/L和(44.7±4.3)μg/L,差异有显著性(t=2.465,P<0.05)。CCR5 AD患者为1.284±0.088,健康对照为1.133±0.075,差异有显著性(t=2.752,P<0.05)。结论RANTES和MIP-1α及特异性受体CCR5在特应性皮炎患者中均显著增高,差异有显著性,在AD的发病中可能起重要作用。展开更多
基金supported by the grant from the Ministry of Science and Technology of China (No. 2005CB1208)
文摘Rice tillering is one of the most important agronomic traits that determine grain yields.Our previous study has demonstrated that the MONOCULM1 （MOC1） gene is a key component that controls the formation of rice tiller buds.To further elucidate the molecular mechanism of MOC1 involved in the regulation of rice tillering,we performed a yeast-two-hybrid screening to identify MOC1 interacting proteins （MIPs）.Here we reported that MIP1 interacted with MOC1 both in vitro and in vivo.The overexpression of MIP1 resulted in enhanced tillering and reduced plant height.In-depth characterization of the context of MIP1 and MOC1 would further our understanding of molecular regulatory mechanisms of rice tillering.
文摘目的探讨调节正常T细胞表达和分泌活性因子(Regulated on activation normal T cell expressed and secre-ted,RANTES),巨噬细胞炎性蛋白(Macrophage inflammatory protein-1,MIP-1α)及趋化因子受体CCR5在特应性皮炎(AD)发病中的作用。方法采集30例AD患者及10例健康对照者血液,分离血清和外周血单个核细胞(PBMC),双抗体夹心ELISA法检测PBMC产生的RANTES、MIP-1α含量,荧光定量PCR检测外周血PBMC表达的CCR5 mRNA水平。结果特应性皮炎患者与健康对照者血清RANTES含量分别为(55.7±3.4)μg/L和(35.6±1.8)μg/L,差异有显著性(t=3.036,P<0.01),且RANTES水平与SCORAD呈正相关(r=0.889,P<0.05);MIP-1α含量分别为(51.8±3.6)μg/L和(44.7±4.3)μg/L,差异有显著性(t=2.465,P<0.05)。CCR5 AD患者为1.284±0.088,健康对照为1.133±0.075,差异有显著性(t=2.752,P<0.05)。结论RANTES和MIP-1α及特异性受体CCR5在特应性皮炎患者中均显著增高,差异有显著性,在AD的发病中可能起重要作用。
