基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time of flight mass spectrometr y,M A LDI-TOF MS)是近年来开始应用于临床微生物检验领域的一种新技术。本文将系统综述MALDI-TOF MS技术的检测原理...基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time of flight mass spectrometr y,M A LDI-TOF MS)是近年来开始应用于临床微生物检验领域的一种新技术。本文将系统综述MALDI-TOF MS技术的检测原理及特点,在临床微生物鉴定、细菌分型、同源性分析和细菌耐药检测中的应用。MALDI-TOF MS技术的采用不仅明显缩短了临床微生物检验的TAT时间,更好地服务于感染性疾病的诊断与抗菌药物合理使用,同时也为临床微生物检验的自动化打下了基础。展开更多
An azyl group of ethylene diamine was protected by N-tert-butoxycarbonyl.The remaining azyl group then successively reacted with methyl acrylate, ethylene diamine and methyl acrylate again to afford the compound Boc-N...An azyl group of ethylene diamine was protected by N-tert-butoxycarbonyl.The remaining azyl group then successively reacted with methyl acrylate, ethylene diamine and methyl acrylate again to afford the compound Boc-NH 2-(B-COOCH 3) 4 3 (AB-4).The ester groups and the Boc-end group of 3 were transferred to carboxylate 4 and azyl group 5 via hydrolyzation and deprotection respectively.The fan-shape dendron molecules with 16 exterior groups 6 were obtained by condensation of 4 and 5 in the presence of DCC.The dumbbell-like dendrimer molecules 7 could expediently prepared by coupling the dendrons with two functional groups of hexanedioyl chloride.The paper describes the synthesis procedures,reports the characterization results of FTIR、 1H-NMR、 13C-NMR and MALDI-TOF.Comparing with the PAMAM dendrimers from the divergent method, the synthesized compounds possess accurate structures and few defections.展开更多
OBJECTIVE: To investigate mechanism-based anti-anxiety effects of Shudihuang (Radix Rehmanniae Preparata) polysaccharides (RRPPs) through two-dimensional electrophoresis (2-DE) analysis with mass spectrometry (MS) of ...OBJECTIVE: To investigate mechanism-based anti-anxiety effects of Shudihuang (Radix Rehmanniae Preparata) polysaccharides (RRPPs) through two-dimensional electrophoresis (2-DE) analysis with mass spectrometry (MS) of hippocampus proteins in rats treated with monosodium L-glutamate (MSG).METHODS: MSG (4 g/kg) or normal saline (NS) was injected subcutaneously into infant male rats on days 2, 4, 6, 8, 10 after birth. MSG-treated rats at 8 weeks old were given NS, diazepam, or RRPPs daily for seven consecutive days by intragastric administration, while NS-treated rats given the same volume of NS. Elevated plus maze (EPM) and light/ dark transition (LDT) tests were used to observe anti-anxiety effects of RRPPs at 1 h after the last administration. After EPM and LDT tests, hippocampus tissues were excised on ice rapidly from the brains of rats. Thereafter, 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) were used for detecting differential proteins in hippocampus of rats so as to explore the potential mechanisms. RESULTS: RRPPs (200, 400 mg/kg) could significantly inhibit MSG-induced decrease of time and entries percentages in open zones in EPM test and numbers of light-dark transition in LDT test. Further analysis of 2-DE and MALDI-TOF/MS indicated that β-synuclein, protein DJ-1, peroxiredoxin-2, peroxiredoxin-6, dimethylarginine dimethylaminohydrolase 1 (DDAH-1) and iron-sulfur proteins were all found to be down-regulated significantly in MSG-treated rats, while such down-regulation was significantly inhibited after treatment with RRPPs. CONCLUSION: RRPPs showed anti-anxiety effects and potential mechanisms might be related to inhibiting MSG-induced down-regulation of β-synuclein, DJ-1, peroxiredoxin-2, peroxiredoxin-6, DDAH-1 and iron-sulfur proteins in hippocampus of rats.展开更多
基质辅助激光解吸电离飞行时间质谱(Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry,MALDI-TOF MS)技术在食品、医药、化学等领域得到了广泛的应用.综述了MALDI-TOF MS技术的检测原理及特点,以及其在...基质辅助激光解吸电离飞行时间质谱(Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry,MALDI-TOF MS)技术在食品、医药、化学等领域得到了广泛的应用.综述了MALDI-TOF MS技术的检测原理及特点,以及其在食品微生物鉴定、分型、溯源分析中的应用.MALDI-TOF MS技术在微生物检验检测上具有快速、准确、高效等优点,随着样本处理方式标准化、数据库的完善化、生物信息学工具使用化等问题的解决,这一技术将成为微生物检测鉴定领域的重要辅助工具.展开更多
尿路感染是临床最常见的感染性疾病之一,也是医院获得性感染的主要病因[1].目前国内外诊断尿路感染的“金标准”仍是清洁中段尿培养[2],而此种方法常常需要2～5d才能得出细菌鉴定及药敏结果,不能及时为临床诊断治疗提供病原学依据.基质...尿路感染是临床最常见的感染性疾病之一,也是医院获得性感染的主要病因[1].目前国内外诊断尿路感染的“金标准”仍是清洁中段尿培养[2],而此种方法常常需要2～5d才能得出细菌鉴定及药敏结果,不能及时为临床诊断治疗提供病原学依据.基质辅助激光解析电离飞行时间质谱（matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF MS）是近些年来发展起来的一种新型软电离有机质谱,可以根据细菌的组分获得其肽质量指纹谱检测并鉴定细菌,其方便、快速、准确的优点对微生物的鉴定具有重要意义[3-5].展开更多
Tumor metastasis is the dominant cause of death in cancer patients. However, the molecular and cellular mechanisms underlying tumor metastasis are still elusive. The identi?cation of protein molecules with their expre...Tumor metastasis is the dominant cause of death in cancer patients. However, the molecular and cellular mechanisms underlying tumor metastasis are still elusive. The identi?cation of protein molecules with their expressions correlated to the metastatic process would help to understand the metastatic mechanisms and thus facilitate the development of strategies for the therapeutic interventions and clini- cal management of cancer. Proteomics is a systematic research approach aiming to provide the global characterization of protein expression and function under given conditions. Proteomic technology has been widely used in biomarker discovery and pathogenetic studies including tumor metastasis. This article provides a brief review of the application of proteomics in identifying molecular factors in tumor metastasis process. The combination of proteomics with other experimental ap- proaches in biochemistry, cell biology, molecular genetics and chemistry, together with the development of new technologies and improvements in existing method- ologies will continue to extend its application in studying cancer metastasis.展开更多
文摘基质辅助激光解吸电离飞行时间质谱(matrix-assisted laser desorption/ionization time of flight mass spectrometr y,M A LDI-TOF MS)是近年来开始应用于临床微生物检验领域的一种新技术。本文将系统综述MALDI-TOF MS技术的检测原理及特点,在临床微生物鉴定、细菌分型、同源性分析和细菌耐药检测中的应用。MALDI-TOF MS技术的采用不仅明显缩短了临床微生物检验的TAT时间,更好地服务于感染性疾病的诊断与抗菌药物合理使用,同时也为临床微生物检验的自动化打下了基础。
文摘An azyl group of ethylene diamine was protected by N-tert-butoxycarbonyl.The remaining azyl group then successively reacted with methyl acrylate, ethylene diamine and methyl acrylate again to afford the compound Boc-NH 2-(B-COOCH 3) 4 3 (AB-4).The ester groups and the Boc-end group of 3 were transferred to carboxylate 4 and azyl group 5 via hydrolyzation and deprotection respectively.The fan-shape dendron molecules with 16 exterior groups 6 were obtained by condensation of 4 and 5 in the presence of DCC.The dumbbell-like dendrimer molecules 7 could expediently prepared by coupling the dendrons with two functional groups of hexanedioyl chloride.The paper describes the synthesis procedures,reports the characterization results of FTIR、 1H-NMR、 13C-NMR and MALDI-TOF.Comparing with the PAMAM dendrimers from the divergent method, the synthesized compounds possess accurate structures and few defections.
基金Supported by National Natural Science Foundation of China(30772794)National Science Foundation for Post-doctoral Scientists of China(2012M521412)Program for Innovative Research Team(in Science and Technology)in Henan College of Chinese Medicine(2011XCXTD01)
文摘OBJECTIVE: To investigate mechanism-based anti-anxiety effects of Shudihuang (Radix Rehmanniae Preparata) polysaccharides (RRPPs) through two-dimensional electrophoresis (2-DE) analysis with mass spectrometry (MS) of hippocampus proteins in rats treated with monosodium L-glutamate (MSG).METHODS: MSG (4 g/kg) or normal saline (NS) was injected subcutaneously into infant male rats on days 2, 4, 6, 8, 10 after birth. MSG-treated rats at 8 weeks old were given NS, diazepam, or RRPPs daily for seven consecutive days by intragastric administration, while NS-treated rats given the same volume of NS. Elevated plus maze (EPM) and light/ dark transition (LDT) tests were used to observe anti-anxiety effects of RRPPs at 1 h after the last administration. After EPM and LDT tests, hippocampus tissues were excised on ice rapidly from the brains of rats. Thereafter, 2-DE and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF/MS) were used for detecting differential proteins in hippocampus of rats so as to explore the potential mechanisms. RESULTS: RRPPs (200, 400 mg/kg) could significantly inhibit MSG-induced decrease of time and entries percentages in open zones in EPM test and numbers of light-dark transition in LDT test. Further analysis of 2-DE and MALDI-TOF/MS indicated that β-synuclein, protein DJ-1, peroxiredoxin-2, peroxiredoxin-6, dimethylarginine dimethylaminohydrolase 1 (DDAH-1) and iron-sulfur proteins were all found to be down-regulated significantly in MSG-treated rats, while such down-regulation was significantly inhibited after treatment with RRPPs. CONCLUSION: RRPPs showed anti-anxiety effects and potential mechanisms might be related to inhibiting MSG-induced down-regulation of β-synuclein, DJ-1, peroxiredoxin-2, peroxiredoxin-6, DDAH-1 and iron-sulfur proteins in hippocampus of rats.
文摘基质辅助激光解吸电离飞行时间质谱(Matrix-Assisted Laser Desorption Ionization Time of Flight Mass Spectrometry,MALDI-TOF MS)技术在食品、医药、化学等领域得到了广泛的应用.综述了MALDI-TOF MS技术的检测原理及特点,以及其在食品微生物鉴定、分型、溯源分析中的应用.MALDI-TOF MS技术在微生物检验检测上具有快速、准确、高效等优点,随着样本处理方式标准化、数据库的完善化、生物信息学工具使用化等问题的解决,这一技术将成为微生物检测鉴定领域的重要辅助工具.
文摘目前,国内大多数临床微生物实验室仍采用传统鉴定方法,如API或Vitek系统,但耗时、高成本及鉴定能力有限是显而易见的制约瓶颈.16S rDNA测序可直接进行无菌标本微生物鉴定,但方法的标准化、标本前处理以及时效性均未得到解决,因此难以成为常规技术.2006年,Fox[1]在Journal of Clinical Microbiology上发表了第一篇有关质谱技术在临床微生物应用的文章,引起同行关注,他认为该技术鉴定细菌还有许多技术难题要克服,不易成为常规方法,但是2009年后微生物质谱技术迅速风靡欧洲、北美和亚洲的一些国家.
文摘尿路感染是临床最常见的感染性疾病之一,也是医院获得性感染的主要病因[1].目前国内外诊断尿路感染的“金标准”仍是清洁中段尿培养[2],而此种方法常常需要2～5d才能得出细菌鉴定及药敏结果,不能及时为临床诊断治疗提供病原学依据.基质辅助激光解析电离飞行时间质谱（matrix-assisted laser desorption/ionization time of flight mass spectrometry,MALDI-TOF MS）是近些年来发展起来的一种新型软电离有机质谱,可以根据细菌的组分获得其肽质量指纹谱检测并鉴定细菌,其方便、快速、准确的优点对微生物的鉴定具有重要意义[3-5].
基金supported by Hong Kong Research Grants Council Grants HKU 7227/02M(to Q.Y.H.),HKU 7218/02M and HKU 7395/03M(toJ.F.C.)the Department of Chemistry,and the Areasof Excellence scheme of Hong Kong University GrantsCommittee
文摘Tumor metastasis is the dominant cause of death in cancer patients. However, the molecular and cellular mechanisms underlying tumor metastasis are still elusive. The identi?cation of protein molecules with their expressions correlated to the metastatic process would help to understand the metastatic mechanisms and thus facilitate the development of strategies for the therapeutic interventions and clini- cal management of cancer. Proteomics is a systematic research approach aiming to provide the global characterization of protein expression and function under given conditions. Proteomic technology has been widely used in biomarker discovery and pathogenetic studies including tumor metastasis. This article provides a brief review of the application of proteomics in identifying molecular factors in tumor metastasis process. The combination of proteomics with other experimental ap- proaches in biochemistry, cell biology, molecular genetics and chemistry, together with the development of new technologies and improvements in existing method- ologies will continue to extend its application in studying cancer metastasis.
