To explore the mechanism of the inhibition of HIV-1 by Mycoplasma fermentans, culture supernatants and thallodic proteins from M.fermentans PG18 were prepared and the protein components of the supernatants were purifi...To explore the mechanism of the inhibition of HIV-1 by Mycoplasma fermentans, culture supernatants and thallodic proteins from M.fermentans PG18 were prepared and the protein components of the supernatants were purified with high performance liquid chromatography (HPLC).The inhibitory activities to reverse transcriptase (RT) and the nuclease activities were detected; the influence of M.fermentans on IL-10 secretion by both normal and HIV-1 infected human PBMC were determined, and the inhibitory effect of rhIL-10 on HIV-1 replication was detected with ELISA method. The results showed that the purified proteins with a molecular weight of 67-100 kDa or 10-25 kDa showed a 36% or 34% in hibitory activity to RT and partial nuclease activity. The thallodic protein could induce both normal and HIV-1 infected PBMC to secret IL-10 remarkably, and to the latter, this effect was more apparent. While rhIL-10 could inhibit replication of HIV-1 in PBMC in vitro in a dose-dependant manner. It concludes that the inhibitory effect of the M.fermentans PG18 culture supernatants on RT and the promoting effect of PG18 thallodic protein on IL-10 secretion in PBMC explain the mechanisms of inhibition to HIV-1 by M.fermentans PG18.展开更多
This study compares the kinetics of adherence of both the prototype strain PG-18 and the recently clinically isolated strain P-140 of Mycoplasma fermentans to HEp-2 cells. M. fermentans may induce respiratory and geni...This study compares the kinetics of adherence of both the prototype strain PG-18 and the recently clinically isolated strain P-140 of Mycoplasma fermentans to HEp-2 cells. M. fermentans may induce respiratory and genitourinary tract infections. We investigated the presence of putative adhesins from strain P-140. A surface protein of 47 kDa, labeled SP47, isolated from M. fermentans P-140, seems to be involved in acterial adherence on HEp-2 cells;this study could allow helping understand the interaction between mycoplasmas and their hosts. Anti-SP47 antibodies inhibited the formation of bacterial clusters and adherence to cultured cells, quantified by ELISA. M. fermentans P-140 was more efficient at adhering to cultured HEp-2 cells than PG-18.展开更多
目的:探究非酿酒酵母与酿酒酵母混合发酵过程中酵母的生长状况及酯酶活性的变化规律。方法:通过测定川南白酒窖池中分离的非酿酒酵母在液体发酵培养基中的酯酶活性,从中优选1株高产酯酶的酵母,经26S r DNA D1/D2区域序列分析鉴定种属。...目的:探究非酿酒酵母与酿酒酵母混合发酵过程中酵母的生长状况及酯酶活性的变化规律。方法:通过测定川南白酒窖池中分离的非酿酒酵母在液体发酵培养基中的酯酶活性,从中优选1株高产酯酶的酵母,经26S r DNA D1/D2区域序列分析鉴定种属。随后利用模拟葡萄汁进行优选酵母和酿酒酵母的混合发酵,设立同时接种、提前48 h接种和提前96 h接种优选酵母3个实验处理,以两株酵母的纯发酵为对照,研究发酵过程中的酵母生长动力学及不同碳链长度底物(C_2~C_8)对应的酯酶活性变化。结果:优选的非酿酒酵母为发酵毕赤酵母。酵母生长动力学研究发现,单一酿酒酵母的发酵速率最快,但混合发酵中的酵母数量更多,尤其是提前96 h接种。模拟发酵处理中,同时接种处理的酯酶活性累积量最大(574 m U/mL),酯酶活性在C_2~C_8有底物特异性,其中C8酯酶活性累积量最高(178~227 m U/mL),C_2酯酶活性累积量最低(46~66 m U/mL)。结合发酵动力学与发酵中酯酶活性变化,发现酯酶活性与酵母的生长状况有关,当酵母生长达到稳定期,酯酶活性达到最大值。结论:优选酵母与酿酒酵母的同时接种发酵具有较高的C4~C8酯酶活性累积量,有增香酿造的应用潜力。展开更多
基金This study was supported by a grant from the National Natural Science Foundation of China (No. 39770678)
文摘To explore the mechanism of the inhibition of HIV-1 by Mycoplasma fermentans, culture supernatants and thallodic proteins from M.fermentans PG18 were prepared and the protein components of the supernatants were purified with high performance liquid chromatography (HPLC).The inhibitory activities to reverse transcriptase (RT) and the nuclease activities were detected; the influence of M.fermentans on IL-10 secretion by both normal and HIV-1 infected human PBMC were determined, and the inhibitory effect of rhIL-10 on HIV-1 replication was detected with ELISA method. The results showed that the purified proteins with a molecular weight of 67-100 kDa or 10-25 kDa showed a 36% or 34% in hibitory activity to RT and partial nuclease activity. The thallodic protein could induce both normal and HIV-1 infected PBMC to secret IL-10 remarkably, and to the latter, this effect was more apparent. While rhIL-10 could inhibit replication of HIV-1 in PBMC in vitro in a dose-dependant manner. It concludes that the inhibitory effect of the M.fermentans PG18 culture supernatants on RT and the promoting effect of PG18 thallodic protein on IL-10 secretion in PBMC explain the mechanisms of inhibition to HIV-1 by M.fermentans PG18.
基金M.Rosales-Pérez was supported by a scholarship No.91996 from Consejo Nacional de Ciencia y Tecnología(CONACYT)México.
文摘This study compares the kinetics of adherence of both the prototype strain PG-18 and the recently clinically isolated strain P-140 of Mycoplasma fermentans to HEp-2 cells. M. fermentans may induce respiratory and genitourinary tract infections. We investigated the presence of putative adhesins from strain P-140. A surface protein of 47 kDa, labeled SP47, isolated from M. fermentans P-140, seems to be involved in acterial adherence on HEp-2 cells;this study could allow helping understand the interaction between mycoplasmas and their hosts. Anti-SP47 antibodies inhibited the formation of bacterial clusters and adherence to cultured cells, quantified by ELISA. M. fermentans P-140 was more efficient at adhering to cultured HEp-2 cells than PG-18.
文摘目的:探究非酿酒酵母与酿酒酵母混合发酵过程中酵母的生长状况及酯酶活性的变化规律。方法:通过测定川南白酒窖池中分离的非酿酒酵母在液体发酵培养基中的酯酶活性,从中优选1株高产酯酶的酵母,经26S r DNA D1/D2区域序列分析鉴定种属。随后利用模拟葡萄汁进行优选酵母和酿酒酵母的混合发酵,设立同时接种、提前48 h接种和提前96 h接种优选酵母3个实验处理,以两株酵母的纯发酵为对照,研究发酵过程中的酵母生长动力学及不同碳链长度底物(C_2~C_8)对应的酯酶活性变化。结果:优选的非酿酒酵母为发酵毕赤酵母。酵母生长动力学研究发现,单一酿酒酵母的发酵速率最快,但混合发酵中的酵母数量更多,尤其是提前96 h接种。模拟发酵处理中,同时接种处理的酯酶活性累积量最大(574 m U/mL),酯酶活性在C_2~C_8有底物特异性,其中C8酯酶活性累积量最高(178~227 m U/mL),C_2酯酶活性累积量最低(46~66 m U/mL)。结合发酵动力学与发酵中酯酶活性变化,发现酯酶活性与酵母的生长状况有关,当酵母生长达到稳定期,酯酶活性达到最大值。结论:优选酵母与酿酒酵母的同时接种发酵具有较高的C4~C8酯酶活性累积量,有增香酿造的应用潜力。
