AIM: To investigate the mechanism of resveratrol underlying the microcirculation disorder and lung injury following severe acute pancreatitis (SAP). METHODS: Twenty-four rats were divided into 3 groups (SAP, sham and ...AIM: To investigate the mechanism of resveratrol underlying the microcirculation disorder and lung injury following severe acute pancreatitis (SAP). METHODS: Twenty-four rats were divided into 3 groups (SAP, sham and resveratrol groups) randomly. SAP model was established by injecting 4% sodium taurocholate l mL/kg through puncturing pancreatic ducts. Sham (control) group (8 rats) was established by turning over the duodenum. Resveratrol was given at 0.1 mg/kg b.m. intraperitoneally. Rats were sacrificed 9 h after SAP was induced. Blood samples were obtained for hemorrheological examination. Lung tissues were used for pathological observation, and examination of microvascular permeability, dry/wet ratio and myeloperoxidase (MPO) activity. Gene expression of intercellular adhesion molecule-1 (ICAM-1) was detected by RT-PCR. RESULTS: Compared with SAP group, resveratrol relieved the edema and infiltration of leukocytes in the lungs. Resveratrol improved markers of hemorrheology: high VTB (5.77±1.18 mPas vs9.49±1.34 mPas), low VTB (16.12±3.20 mPas vs30.91±7.28 mPas), PV (4.69±1.68 mPas vs 8.00±1.34 mPas), BSR (1.25±0.42 mm/h vs50.03±0.03 mm/h), VPC (54.67±3.08% vs 62.17±3.39%), fibrinogen (203.2?7.8 g/ L vs 51.3±19.1 g/L), original hemolysis (0.45±0.02 vs 0.49±0.02), and complete hemolysis (0.41±0.02 vs 0.43±0.02) (P<0.05). Resveratrol decreased the OD ratio of ICAM-1 gene (0.800±0.03 vs 1.188±0.10), dry/wet ratio (0.74±0.02 vs 0.77±0.03), microvascular permeability (0.079±0.006 vs 0.112±0.004) and MPO activity (4.42±0.32 vs 5.03±0.51) significantly (P<0.05). CONCLUSION: Resveratrol can improve the microcirculation disorder of the lung by decreasing leukocyte-endothelial interaction, reducing blood viscosity, improving the decrease of blood flow, and stabilizing erythrocytes in SAP rats. It may be a potential candidate to treat SAP and its severe complications (ALI).展开更多
目的探讨心肺再灌注早期循环血白细胞过滤对体外循环(Extracorporeal c ircu lation,ECC)心瓣膜置换术患者的肺保护作用。方法52例心瓣膜置换术患者,随机分成实验组(EG)和对照组(CG)。实验组在动脉旁路上并行安装白细胞滤器(Pall LG-6)...目的探讨心肺再灌注早期循环血白细胞过滤对体外循环(Extracorporeal c ircu lation,ECC)心瓣膜置换术患者的肺保护作用。方法52例心瓣膜置换术患者,随机分成实验组(EG)和对照组(CG)。实验组在动脉旁路上并行安装白细胞滤器(Pall LG-6),白细胞滤器开放时间在心肺再灌注前5 m in^10 m in至ECC结束;对照组仅用ECC常规动脉滤器。观察术中及术后循环血白细胞计数、再灌注肺内白细胞隔离、ECC前后肺动脉压改变、机械辅助通气和动脉血气分析。结果①在心肺再灌注前,白细胞过滤可显著降低循环血中的白细胞计数;②再灌注15 m in时白细胞过滤对肺内白细胞隔离的影响并不明显;③白细胞过滤可缓和ECC后肺动脉压力的上升;④白细胞过滤可显著降低术后呼吸指数(R I)和提高机械通气氧合指数(O I)。结论再灌注前及再灌注早期循环血白细胞过滤可明显改善心瓣膜置换术患者术后肺功能。展开更多
Objective To evaluate the effects of aerosolized prostaglandin E1（PGE1） inhaled to ventilated lung on hemodynamics and oxygenation during one-lung ventilation（OLV）.Methods Eighteen pigs,weighing 1417 kg,were preme...Objective To evaluate the effects of aerosolized prostaglandin E1（PGE1） inhaled to ventilated lung on hemodynamics and oxygenation during one-lung ventilation（OLV）.Methods Eighteen pigs,weighing 1417 kg,were premedicated with diazepam,ketamine and atropine and intubated and mechanically ventilated.A catheter was placed in the right femoral artery for arterial pressure monitoring and blood sampling.Swan-Ganz catheter was inserted just into the main right pulmonary artery via the left femoral vein.Anesthesia was maintained by infusion of ketamine,procaine,and succinylcholine.The lungs were initially ventilated at a rate of 15 bpm.Tidal volume was adjusted between 1215 ml · kg-1 to achieve PaCO2 of 4.9-5.7 kPa.The FiO2 was set to 0.7.After established OLV,the respiratory rate was increased to 20 bpm and minute volume was congruously maintained throughout the experiment.Following the baseline measurement of all parameters,OLV was established by clamping the left bronchia.Eighteen pigs were divided randomly into three groups: the infused PGE1 group（A,n=6）,the inhaled PGE1 group（B,n=6） and the timecourse control group（C,n=6）.After 60 min OLV,PGE1 was infused into the right pulmonary artery in group A via an infusion pump,or inhaled to ventilated lung in group B via an aerosolized inhalant with an initial dose of 0.01 μg·kg-1 ·min-1 which was gradually increased to 0.02 μg·kg-1·min-1,0.04 μg·kg-1·min-1,0.06 μg·kg-1·min-1,0.08 μg·kg-1·min-1,0.1 μg·kg-1·min-1at 20-min intervals.After the PGE1 administration was stopped,all parameters were recorded at 20,40,and 60 min.The group C received the same amount of normal saline infusion into the right pulmonary artery and inhaled into ventilated lung during the whole periods.Hemodynamic variables and respiratory function parameters were measured at each step.Results One-lung ventilation increased MPAP、PVR、SVR、PIP and Qs/Qt,and reduced CO、Cdyn and PaO2 significantly（P<0.05）in all animals.During selective infusion or inhalation of 展开更多
基金Supported by the National Natural Science Foundation of China, No. 30371398
文摘AIM: To investigate the mechanism of resveratrol underlying the microcirculation disorder and lung injury following severe acute pancreatitis (SAP). METHODS: Twenty-four rats were divided into 3 groups (SAP, sham and resveratrol groups) randomly. SAP model was established by injecting 4% sodium taurocholate l mL/kg through puncturing pancreatic ducts. Sham (control) group (8 rats) was established by turning over the duodenum. Resveratrol was given at 0.1 mg/kg b.m. intraperitoneally. Rats were sacrificed 9 h after SAP was induced. Blood samples were obtained for hemorrheological examination. Lung tissues were used for pathological observation, and examination of microvascular permeability, dry/wet ratio and myeloperoxidase (MPO) activity. Gene expression of intercellular adhesion molecule-1 (ICAM-1) was detected by RT-PCR. RESULTS: Compared with SAP group, resveratrol relieved the edema and infiltration of leukocytes in the lungs. Resveratrol improved markers of hemorrheology: high VTB (5.77±1.18 mPas vs9.49±1.34 mPas), low VTB (16.12±3.20 mPas vs30.91±7.28 mPas), PV (4.69±1.68 mPas vs 8.00±1.34 mPas), BSR (1.25±0.42 mm/h vs50.03±0.03 mm/h), VPC (54.67±3.08% vs 62.17±3.39%), fibrinogen (203.2?7.8 g/ L vs 51.3±19.1 g/L), original hemolysis (0.45±0.02 vs 0.49±0.02), and complete hemolysis (0.41±0.02 vs 0.43±0.02) (P<0.05). Resveratrol decreased the OD ratio of ICAM-1 gene (0.800±0.03 vs 1.188±0.10), dry/wet ratio (0.74±0.02 vs 0.77±0.03), microvascular permeability (0.079±0.006 vs 0.112±0.004) and MPO activity (4.42±0.32 vs 5.03±0.51) significantly (P<0.05). CONCLUSION: Resveratrol can improve the microcirculation disorder of the lung by decreasing leukocyte-endothelial interaction, reducing blood viscosity, improving the decrease of blood flow, and stabilizing erythrocytes in SAP rats. It may be a potential candidate to treat SAP and its severe complications (ALI).
文摘目的探讨心肺再灌注早期循环血白细胞过滤对体外循环(Extracorporeal c ircu lation,ECC)心瓣膜置换术患者的肺保护作用。方法52例心瓣膜置换术患者,随机分成实验组(EG)和对照组(CG)。实验组在动脉旁路上并行安装白细胞滤器(Pall LG-6),白细胞滤器开放时间在心肺再灌注前5 m in^10 m in至ECC结束;对照组仅用ECC常规动脉滤器。观察术中及术后循环血白细胞计数、再灌注肺内白细胞隔离、ECC前后肺动脉压改变、机械辅助通气和动脉血气分析。结果①在心肺再灌注前,白细胞过滤可显著降低循环血中的白细胞计数;②再灌注15 m in时白细胞过滤对肺内白细胞隔离的影响并不明显;③白细胞过滤可缓和ECC后肺动脉压力的上升;④白细胞过滤可显著降低术后呼吸指数(R I)和提高机械通气氧合指数(O I)。结论再灌注前及再灌注早期循环血白细胞过滤可明显改善心瓣膜置换术患者术后肺功能。
文摘Objective To evaluate the effects of aerosolized prostaglandin E1（PGE1） inhaled to ventilated lung on hemodynamics and oxygenation during one-lung ventilation（OLV）.Methods Eighteen pigs,weighing 1417 kg,were premedicated with diazepam,ketamine and atropine and intubated and mechanically ventilated.A catheter was placed in the right femoral artery for arterial pressure monitoring and blood sampling.Swan-Ganz catheter was inserted just into the main right pulmonary artery via the left femoral vein.Anesthesia was maintained by infusion of ketamine,procaine,and succinylcholine.The lungs were initially ventilated at a rate of 15 bpm.Tidal volume was adjusted between 1215 ml · kg-1 to achieve PaCO2 of 4.9-5.7 kPa.The FiO2 was set to 0.7.After established OLV,the respiratory rate was increased to 20 bpm and minute volume was congruously maintained throughout the experiment.Following the baseline measurement of all parameters,OLV was established by clamping the left bronchia.Eighteen pigs were divided randomly into three groups: the infused PGE1 group（A,n=6）,the inhaled PGE1 group（B,n=6） and the timecourse control group（C,n=6）.After 60 min OLV,PGE1 was infused into the right pulmonary artery in group A via an infusion pump,or inhaled to ventilated lung in group B via an aerosolized inhalant with an initial dose of 0.01 μg·kg-1 ·min-1 which was gradually increased to 0.02 μg·kg-1·min-1,0.04 μg·kg-1·min-1,0.06 μg·kg-1·min-1,0.08 μg·kg-1·min-1,0.1 μg·kg-1·min-1at 20-min intervals.After the PGE1 administration was stopped,all parameters were recorded at 20,40,and 60 min.The group C received the same amount of normal saline infusion into the right pulmonary artery and inhaled into ventilated lung during the whole periods.Hemodynamic variables and respiratory function parameters were measured at each step.Results One-lung ventilation increased MPAP、PVR、SVR、PIP and Qs/Qt,and reduced CO、Cdyn and PaO2 significantly（P<0.05）in all animals.During selective infusion or inhalation of
