本试验旨在研究低钙日粮中添加依普黄酮对笼养蛋鸡骨质疏松症病鸡骨组织显微结构和形态计量学的影响。200只24周龄健康海兰褐蛋鸡随机分为4组:对照组(基础日粮中含钙3.55%)、低钙组(日粮中含钙1.27%)、试验Ⅰ组(8mg.kg-1依普黄酮+低钙日...本试验旨在研究低钙日粮中添加依普黄酮对笼养蛋鸡骨质疏松症病鸡骨组织显微结构和形态计量学的影响。200只24周龄健康海兰褐蛋鸡随机分为4组:对照组(基础日粮中含钙3.55%)、低钙组(日粮中含钙1.27%)、试验Ⅰ组(8mg.kg-1依普黄酮+低钙日粮)和试验Ⅱ组(20mg.kg-1依普黄酮+低钙日粮),试验期60d。试验结束时,每组剖杀10只鸡,取左胫骨中段脱钙骨切片在OLYMPUS BX41型金相显微镜下观察骨组织形态结构的变化,并以Image-Pro Plus 6.0图像分析软件测定骨组织形态计量学的相关静态参数。结果表明:骨显微结构观察,与对照组相比,低钙组蛋鸡胫骨皮质骨出现许多吸收腔,在吸收腔内有大量破骨细胞,髓质骨骨小梁表面破骨细胞增多,骨吸收大于骨形成,导致骨质疏松;试验Ⅰ组蛋鸡胫骨皮质骨有吸收腔,吸收腔内和小梁表面可见较多破骨细胞,骨吸收未受抑制;试验Ⅱ组蛋鸡胫骨皮质骨外表面可见大量骨原细胞和成骨细胞,骨形成增强。形态计量学静态参数测定,与低钙组相比,试验Ⅰ、Ⅱ组的骨小梁面积、骨小梁面积百分比均极显著增加(P<0.01);试验Ⅰ、Ⅱ组的骨小梁周长、骨小梁数量高于低钙组,但差异不显著(P>0.05);试验Ⅱ组的骨小梁分离度显著降低(P<0.05)及骨小梁厚度显著增加(P<0.05)。上述结果说明,在低钙日粮中添加20mg.kg-1依普黄酮可使骨重建活动加强,改善笼养蛋鸡的骨代谢及缓解骨质疏松症的发生。展开更多
Dorsal root ganglion (DRG) neurons may be divided into rapidly conducting type A and slowly conducting type C neurons in terms of electrophysiological studies. No report regarding type C neurons and their synaptic act...Dorsal root ganglion (DRG) neurons may be divided into rapidly conducting type A and slowly conducting type C neurons in terms of electrophysiological studies. No report regarding type C neurons and their synaptic activity can be found till now because of difficulties in penetrating and recording type C neurons due to their small-sized cell body. What we and others have reported was mainly large-sized type A neurons and their activity. On the other hand, synaptic or junctional activities of peripheral and central neurons have been successfully studied by blocking action of high magnesium and low calcium perfusion on synaptic transmission and calcium channels. The present study, therefore, aims at finding and recording the type C neurons and their responses to展开更多
目的研究低钙对血清补体C3的影响及其可能的机制。方法雄性Wistar大鼠30只,按体重随机分为低钙组（喂饲0.15%Ca）和正常钙对照组（喂饲0.50%Ca）,每组15只。喂养12w后,麻醉处死大鼠,取组织和血清。采用双向电泳技术分离大鼠血清,联合470...目的研究低钙对血清补体C3的影响及其可能的机制。方法雄性Wistar大鼠30只,按体重随机分为低钙组（喂饲0.15%Ca）和正常钙对照组（喂饲0.50%Ca）,每组15只。喂养12w后,麻醉处死大鼠,取组织和血清。采用双向电泳技术分离大鼠血清,联合4700串联飞行时间质谱仪和蛋白质数据库分析鉴定差异表达蛋白质,并检测各组大鼠血清补体C3等生化指标。为通过人群进一步验证,在性别、年龄匹配的基础上,调查（1~3岁）佝偻病儿童及健康对照儿童,检测血清补体C3等生化指标。用real time PCR技术检测体外低钙培养的HepG2细胞及各组大鼠肝脏补体C3、furin酶mRNA表达情况。结果通过血清蛋白质组学技术筛选出低钙动物模型血清补体C3显著降低。与正常钙对照组（0.30±0.02）g/L比,低钙组大鼠血清补体C3（0.24±0.04）g/L显著降低（P〈0.05）。共调查123名（1~3岁）佝偻病儿童和114名健康对照儿童,与健康对照儿童（1.52±0.10）g/L相比,佝偻病儿童血清补体C3（1.27±0.10）g/L显著降低（P〈0.05）。低钙时HepG2细胞和大鼠肝脏补体C3、furin酶mRNA表达下降。结论低钙可能通过降低furin酶mRNA的表达,导致佝偻病儿童血清补体C3水平降低,机体免疫力下降,增加上呼吸道感染的危险。[营养学报,2012,34（6）：586-590]展开更多
Effects of cobalt ions (Co2+) on horizontal cells in low extracellular calcium were examined in isolated, superfused carp retinas. While 0.1mmol/L Co2+ completely suppressed both rod- and cone-driven horizontal cells ...Effects of cobalt ions (Co2+) on horizontal cells in low extracellular calcium were examined in isolated, superfused carp retinas. While 0.1mmol/L Co2+ completely suppressed both rod- and cone-driven horizontal cells in normal Ringer’s solution, it enhanced light responses of cone horizontal cells in low (0.1mmol/L) calcium. The enhancement of the cone horizontal cell response by Co2+ was not caused by changes in light responsiveness of cone photoreceptors. Moreover, application of 50μmol/L IBMX, an inhibitor of phosphodiester enzyme, reduced the suppressive effect of 0.1 mmol/L Co2+ in normal Ringer’s solution. In consequence, the above-described enhancement of the cone horizontal cell light responsiveness may be due to a depolarization of cones caused by low calcium, which increases the activity of voltage-dependent calcium channels at cone terminals.展开更多
文摘本试验旨在研究低钙日粮中添加依普黄酮对笼养蛋鸡骨质疏松症病鸡骨组织显微结构和形态计量学的影响。200只24周龄健康海兰褐蛋鸡随机分为4组:对照组(基础日粮中含钙3.55%)、低钙组(日粮中含钙1.27%)、试验Ⅰ组(8mg.kg-1依普黄酮+低钙日粮)和试验Ⅱ组(20mg.kg-1依普黄酮+低钙日粮),试验期60d。试验结束时,每组剖杀10只鸡,取左胫骨中段脱钙骨切片在OLYMPUS BX41型金相显微镜下观察骨组织形态结构的变化,并以Image-Pro Plus 6.0图像分析软件测定骨组织形态计量学的相关静态参数。结果表明:骨显微结构观察,与对照组相比,低钙组蛋鸡胫骨皮质骨出现许多吸收腔,在吸收腔内有大量破骨细胞,髓质骨骨小梁表面破骨细胞增多,骨吸收大于骨形成,导致骨质疏松;试验Ⅰ组蛋鸡胫骨皮质骨有吸收腔,吸收腔内和小梁表面可见较多破骨细胞,骨吸收未受抑制;试验Ⅱ组蛋鸡胫骨皮质骨外表面可见大量骨原细胞和成骨细胞,骨形成增强。形态计量学静态参数测定,与低钙组相比,试验Ⅰ、Ⅱ组的骨小梁面积、骨小梁面积百分比均极显著增加(P<0.01);试验Ⅰ、Ⅱ组的骨小梁周长、骨小梁数量高于低钙组,但差异不显著(P>0.05);试验Ⅱ组的骨小梁分离度显著降低(P<0.05)及骨小梁厚度显著增加(P<0.05)。上述结果说明,在低钙日粮中添加20mg.kg-1依普黄酮可使骨重建活动加强,改善笼养蛋鸡的骨代谢及缓解骨质疏松症的发生。
文摘Dorsal root ganglion (DRG) neurons may be divided into rapidly conducting type A and slowly conducting type C neurons in terms of electrophysiological studies. No report regarding type C neurons and their synaptic activity can be found till now because of difficulties in penetrating and recording type C neurons due to their small-sized cell body. What we and others have reported was mainly large-sized type A neurons and their activity. On the other hand, synaptic or junctional activities of peripheral and central neurons have been successfully studied by blocking action of high magnesium and low calcium perfusion on synaptic transmission and calcium channels. The present study, therefore, aims at finding and recording the type C neurons and their responses to
文摘目的研究低钙对血清补体C3的影响及其可能的机制。方法雄性Wistar大鼠30只,按体重随机分为低钙组（喂饲0.15%Ca）和正常钙对照组（喂饲0.50%Ca）,每组15只。喂养12w后,麻醉处死大鼠,取组织和血清。采用双向电泳技术分离大鼠血清,联合4700串联飞行时间质谱仪和蛋白质数据库分析鉴定差异表达蛋白质,并检测各组大鼠血清补体C3等生化指标。为通过人群进一步验证,在性别、年龄匹配的基础上,调查（1~3岁）佝偻病儿童及健康对照儿童,检测血清补体C3等生化指标。用real time PCR技术检测体外低钙培养的HepG2细胞及各组大鼠肝脏补体C3、furin酶mRNA表达情况。结果通过血清蛋白质组学技术筛选出低钙动物模型血清补体C3显著降低。与正常钙对照组（0.30±0.02）g/L比,低钙组大鼠血清补体C3（0.24±0.04）g/L显著降低（P〈0.05）。共调查123名（1~3岁）佝偻病儿童和114名健康对照儿童,与健康对照儿童（1.52±0.10）g/L相比,佝偻病儿童血清补体C3（1.27±0.10）g/L显著降低（P〈0.05）。低钙时HepG2细胞和大鼠肝脏补体C3、furin酶mRNA表达下降。结论低钙可能通过降低furin酶mRNA的表达,导致佝偻病儿童血清补体C3水平降低,机体免疫力下降,增加上呼吸道感染的危险。[营养学报,2012,34（6）：586-590]
基金Project supported by the State Commission of Science and Technology of China, the National Natural Science Foundation of China,the National Eye Institute(USA) and the Human Frontier Science Program.
文摘Effects of cobalt ions (Co2+) on horizontal cells in low extracellular calcium were examined in isolated, superfused carp retinas. While 0.1mmol/L Co2+ completely suppressed both rod- and cone-driven horizontal cells in normal Ringer’s solution, it enhanced light responses of cone horizontal cells in low (0.1mmol/L) calcium. The enhancement of the cone horizontal cell response by Co2+ was not caused by changes in light responsiveness of cone photoreceptors. Moreover, application of 50μmol/L IBMX, an inhibitor of phosphodiester enzyme, reduced the suppressive effect of 0.1 mmol/L Co2+ in normal Ringer’s solution. In consequence, the above-described enhancement of the cone horizontal cell light responsiveness may be due to a depolarization of cones caused by low calcium, which increases the activity of voltage-dependent calcium channels at cone terminals.
