The length,width,thickness,length/width ratio and 100-seed weight of Magnolia officinalis seeds from 15 natural distribution regions in China were measured and their germination rates were tested.The results showed th...The length,width,thickness,length/width ratio and 100-seed weight of Magnolia officinalis seeds from 15 natural distribution regions in China were measured and their germination rates were tested.The results showed that there were significant differences among seeds from different region in seed characters and 100-seed weight.Correlation analysis between seed characters and geographical climatic factors showed that the main variation was of geographical locations.The annual mean temperature,annual precipitation and frost-free period were the leading ecological factors restricting the seed variation.The results of test showed that there were significant differences in germination rates among different seed sources.The seeds from Ningqiang had the highest germination rate,while that from Kaixian the minimum.The seeds from Jingning,Wuyishan and Longsheng had high germination potential.According to cluster analysis on 5 trait-location mean values including seed size and quality and geographical climatic factors,the Magnolia officinalis from 15 natural distribution regions could be divided into 2 provenance regions,i.e.south-east M.officinalis provenance region and north-west provenance region.展开更多
Southern corn rust is one of destructive diseases in maize caused by Puccinia polysora Undrew. A mapping population of tropical sweet corn recombinant inbred lines (RILs) derived from a cross between hA9104 and hA9035...Southern corn rust is one of destructive diseases in maize caused by Puccinia polysora Undrew. A mapping population of tropical sweet corn recombinant inbred lines (RILs) derived from a cross between hA9104 and hA9035 inbred lines were set up to detect quantitative trait loci (QTLs) involved in partial resistance to southern corn rust. Eighty nine RILs were used to evaluate resistance levels using nine-point relative scale (1-9) at Sweet Seeds, Suwan Farm, Thailand include combined analysis. A genetic linkage map was constructed with 157 SSR markers, with a total length of 2123.1 cM, covering 10 chromosomes. Broad-sense heritability of individual location ranged from 0.76 and 0.82 and combined across locations was 0.87. Multiple QTL mapping (MQM) was applied for the identification of the QTLs. Fifteen QTLs were detected on chromosome 1, 2, 5, 6, 9 and 10 in both locations and combined across locations. QTLs on chromosome 1, 5 and 6 were contributed by alleles of resistant parent hA9104 while others were contributed by alleles from the susceptible parent, hA9035. Phenotypic variance of each QTL explained ranged from 6.1% to 41.8% with a total of 69.8% - 81.9%. QTL on chromosome 1, 6 and 10 were stable QTLs detected in both locations.展开更多
A gas production potential method for optimization of gas wellsite locations selection is proposed in terms of the coalbed gas resources volume and the recoverability. The method uses the actual data about reservoirs ...A gas production potential method for optimization of gas wellsite locations selection is proposed in terms of the coalbed gas resources volume and the recoverability. The method uses the actual data about reservoirs in a coalbed gas field in central China to optimize wellsite locations in the studied area in combination with the dynamic data about actual production in the coalbed gas field, selects a favorable subarea for gas wells deployment. The method is established based on the basic properties of coal reservoirs, in combination with the coalbed thickness and the gas content to make an analysis of the gas storage potential of a coal reservoir, as well as resources volume and the permeability of a coal reservoir. This method can be popularized for optimization of wellsite locations in other methane gas development areas or blocks.展开更多
A neck scar contracture can severely and negatively affect the function of mastication, phonic, or breathing and result in neck pain and issues with esthetics. The best way is of course to avoid such contracture by me...A neck scar contracture can severely and negatively affect the function of mastication, phonic, or breathing and result in neck pain and issues with esthetics. The best way is of course to avoid such contracture by means of non-surgical treatment such as use of a growth factor. The basic fibroblastic growth factor is clinically well proven in decreasing scar formation and improving healing. There are numerous reconstructive methods for neck contracture, especially when the lesions are relatively limited in part of the neck. However, a very severe and full circumferential scar contracture requires extensive reconstruction. The thin groin flap is one of the answers and well matches with the tissue texture and maintains the flexibility. Even with extensive burns and delayed reconstructions due to resuscitation first, the groin area is well preserved and can be safely harvested by dual vasculature systems of the superficial circumflex iliac artery and superficial epigastric artery, which warrant more reliability compared to the perforator flaps in this area. More demanding and stringent forms of the neck burn scar contracture are the sequelae of radiation. A radiation burn or radiation injury can be progressing and hard to heal. Adipose-derived stem cells can reverse the scar contracture as the surrounding tissue is softened and can accelerate wound healing. In this review, different types of neck burn scar contracture and reconstructive methods are summarized, including innovative use of bFGF and ADSCs in the management of difficult wound healing and scar contracture.展开更多
Background It is uncertain whether genotypes of Candida albicans （C. albicans） are associated with colonizing body locations or variant conditions of infection. The aim of this study was to investigate whether there...Background It is uncertain whether genotypes of Candida albicans （C. albicans） are associated with colonizing body locations or variant conditions of infection. The aim of this study was to investigate whether there are significant associations between strain genotypes and body sites of infection and to determine the potential pathogenesis of cutaneous candidiasis at multiple locations. Methods A total of 151 strains of C. albicans were isolated from 74 infant patients with cutaneous candidiasis and 61 female patients with vaginal candidiasis. Patients were grouped according to the body sites and underlying conditions of infection. Genotypes were identified by polymerase chain reaction （PCR） of the 25S rDNA and PCR-restriction fragment length polymorphism （RFLP） of ALT repeats digested with EcoRI and Clal. Results Ten genotypes were detected. There were significant differences in genotype frequencies between the two groups. However, we found no clear association between genotypes and the sites of cutaneous infection or the underlying conditions of vaginal candidiasis （VVC）. In addition, strains of C. albicans from multiple cutaneous locations of the same patient had identical genotypes. Conclusions Populations of C. albicans from patients with cutaneous and vaginal candidiasis were genetically different. However, the lack of genetic difference between strains from different body sites with cutaneous infections or from different underlying conditions for VVC suggests no evidence of genotype selection for different skin surfaces or patients with different underlying conditions for VVC.展开更多
Colorectal endoscopic submucosal dissection(ESD) is considered one of the most challenging endoscopic procedures for novice endoscopists. When compared with the stomach, the colon and rectum have a narrower tubular lu...Colorectal endoscopic submucosal dissection(ESD) is considered one of the most challenging endoscopic procedures for novice endoscopists. When compared with the stomach, the colon and rectum have a narrower tubular lumen, greater angulation at the flexures, and a thinner muscle layer. These factors make endoscopic control and maneuverability difficult. ESD of the colorectum was considered more difficult than gastric and esophageal ESD. However, with learning from the experts, practicing, and selecting an appropriate technique,most of colorectal ESD could be performed successfully. Nevertheless, some colorectal locations are extremely specialized either from unique anatomy or given unstable scope position. Accordingly, the objective of this review was to provide endoscopists with an overview of the techniques and outcomes associated with ESD at these special colorectal locations. ESD at the discussed special locations of the ileo-colo-rectum was found to be feasible, and outcomes were comparable to those of ESD performed in non-special locations of the ileocolo-rectum. Practice for skill improvement and awareness of the unique characteristics of each special location is the key to performing successful ESD.展开更多
文摘The length,width,thickness,length/width ratio and 100-seed weight of Magnolia officinalis seeds from 15 natural distribution regions in China were measured and their germination rates were tested.The results showed that there were significant differences among seeds from different region in seed characters and 100-seed weight.Correlation analysis between seed characters and geographical climatic factors showed that the main variation was of geographical locations.The annual mean temperature,annual precipitation and frost-free period were the leading ecological factors restricting the seed variation.The results of test showed that there were significant differences in germination rates among different seed sources.The seeds from Ningqiang had the highest germination rate,while that from Kaixian the minimum.The seeds from Jingning,Wuyishan and Longsheng had high germination potential.According to cluster analysis on 5 trait-location mean values including seed size and quality and geographical climatic factors,the Magnolia officinalis from 15 natural distribution regions could be divided into 2 provenance regions,i.e.south-east M.officinalis provenance region and north-west provenance region.
文摘Southern corn rust is one of destructive diseases in maize caused by Puccinia polysora Undrew. A mapping population of tropical sweet corn recombinant inbred lines (RILs) derived from a cross between hA9104 and hA9035 inbred lines were set up to detect quantitative trait loci (QTLs) involved in partial resistance to southern corn rust. Eighty nine RILs were used to evaluate resistance levels using nine-point relative scale (1-9) at Sweet Seeds, Suwan Farm, Thailand include combined analysis. A genetic linkage map was constructed with 157 SSR markers, with a total length of 2123.1 cM, covering 10 chromosomes. Broad-sense heritability of individual location ranged from 0.76 and 0.82 and combined across locations was 0.87. Multiple QTL mapping (MQM) was applied for the identification of the QTLs. Fifteen QTLs were detected on chromosome 1, 2, 5, 6, 9 and 10 in both locations and combined across locations. QTLs on chromosome 1, 5 and 6 were contributed by alleles of resistant parent hA9104 while others were contributed by alleles from the susceptible parent, hA9035. Phenotypic variance of each QTL explained ranged from 6.1% to 41.8% with a total of 69.8% - 81.9%. QTL on chromosome 1, 6 and 10 were stable QTLs detected in both locations.
文摘A gas production potential method for optimization of gas wellsite locations selection is proposed in terms of the coalbed gas resources volume and the recoverability. The method uses the actual data about reservoirs in a coalbed gas field in central China to optimize wellsite locations in the studied area in combination with the dynamic data about actual production in the coalbed gas field, selects a favorable subarea for gas wells deployment. The method is established based on the basic properties of coal reservoirs, in combination with the coalbed thickness and the gas content to make an analysis of the gas storage potential of a coal reservoir, as well as resources volume and the permeability of a coal reservoir. This method can be popularized for optimization of wellsite locations in other methane gas development areas or blocks.
文摘A neck scar contracture can severely and negatively affect the function of mastication, phonic, or breathing and result in neck pain and issues with esthetics. The best way is of course to avoid such contracture by means of non-surgical treatment such as use of a growth factor. The basic fibroblastic growth factor is clinically well proven in decreasing scar formation and improving healing. There are numerous reconstructive methods for neck contracture, especially when the lesions are relatively limited in part of the neck. However, a very severe and full circumferential scar contracture requires extensive reconstruction. The thin groin flap is one of the answers and well matches with the tissue texture and maintains the flexibility. Even with extensive burns and delayed reconstructions due to resuscitation first, the groin area is well preserved and can be safely harvested by dual vasculature systems of the superficial circumflex iliac artery and superficial epigastric artery, which warrant more reliability compared to the perforator flaps in this area. More demanding and stringent forms of the neck burn scar contracture are the sequelae of radiation. A radiation burn or radiation injury can be progressing and hard to heal. Adipose-derived stem cells can reverse the scar contracture as the surrounding tissue is softened and can accelerate wound healing. In this review, different types of neck burn scar contracture and reconstructive methods are summarized, including innovative use of bFGF and ADSCs in the management of difficult wound healing and scar contracture.
基金This work was partly supported by a grant from the National Natural Science Foundation of China （No. 30571676）.Acknowledgements： We are grateful to Prof. XU Jian-ping （Department of Biology, McMaster University, Canada） for his helpful advice and critical appraisal of this manuscript. We also thank LU Gui-xia, LIU Jin-xia and GONG Kuang-long for help in the collection of strains in this study.
文摘Background It is uncertain whether genotypes of Candida albicans （C. albicans） are associated with colonizing body locations or variant conditions of infection. The aim of this study was to investigate whether there are significant associations between strain genotypes and body sites of infection and to determine the potential pathogenesis of cutaneous candidiasis at multiple locations. Methods A total of 151 strains of C. albicans were isolated from 74 infant patients with cutaneous candidiasis and 61 female patients with vaginal candidiasis. Patients were grouped according to the body sites and underlying conditions of infection. Genotypes were identified by polymerase chain reaction （PCR） of the 25S rDNA and PCR-restriction fragment length polymorphism （RFLP） of ALT repeats digested with EcoRI and Clal. Results Ten genotypes were detected. There were significant differences in genotype frequencies between the two groups. However, we found no clear association between genotypes and the sites of cutaneous infection or the underlying conditions of vaginal candidiasis （VVC）. In addition, strains of C. albicans from multiple cutaneous locations of the same patient had identical genotypes. Conclusions Populations of C. albicans from patients with cutaneous and vaginal candidiasis were genetically different. However, the lack of genetic difference between strains from different body sites with cutaneous infections or from different underlying conditions for VVC suggests no evidence of genotype selection for different skin surfaces or patients with different underlying conditions for VVC.
文摘Colorectal endoscopic submucosal dissection(ESD) is considered one of the most challenging endoscopic procedures for novice endoscopists. When compared with the stomach, the colon and rectum have a narrower tubular lumen, greater angulation at the flexures, and a thinner muscle layer. These factors make endoscopic control and maneuverability difficult. ESD of the colorectum was considered more difficult than gastric and esophageal ESD. However, with learning from the experts, practicing, and selecting an appropriate technique,most of colorectal ESD could be performed successfully. Nevertheless, some colorectal locations are extremely specialized either from unique anatomy or given unstable scope position. Accordingly, the objective of this review was to provide endoscopists with an overview of the techniques and outcomes associated with ESD at these special colorectal locations. ESD at the discussed special locations of the ileo-colo-rectum was found to be feasible, and outcomes were comparable to those of ESD performed in non-special locations of the ileocolo-rectum. Practice for skill improvement and awareness of the unique characteristics of each special location is the key to performing successful ESD.
