White rot fungus Pleurotus djamor H1 was stilly cultured at 28 ℃ under 4 different kinds of LNAS culture solution,the extracellular enzyme solutions were sampled at different interval,O.D.values of the solutions,repr...White rot fungus Pleurotus djamor H1 was stilly cultured at 28 ℃ under 4 different kinds of LNAS culture solution,the extracellular enzyme solutions were sampled at different interval,O.D.values of the solutions,representing manganese peroxidase(MnP),laccase and lignin peroxidase(LiP)activities,were measured spectrophotometrically by monitoring the oxidation of 2,6-DMP at 470 nm,ABTS at 420 nm and veratryl alcohol(VA)at 310 nm,the lignin-degrading enzymes of the fungus and the relationships between enzyme activities and medium composition and substrates were gained.Results indicated that Pleurotus djamor could produce MnP and laccase simultaneously,but no Lip.Substrates wood sawdust and 2,6-DMP could significantly enhanced MnP and laccase activities,the biggest Mnp and laccase activity were 5 U·L-1.and 38 U·L-1 when no substrates were added,and 69 U·L-1 and 205 U·L-1 when substrates added.The paper offered a basic ligninolytic enzymology of Pleurotus djamor for future utilization of the enzymes and further study on MnP and laccase mechanism of the fungus.展开更多
A 120-day experiment was conducted to compare the removal of polycyclic aromatic hydrocarbons(PAHs) from agricultural soil after natural attenuation(NA), phytoremediation(P), mycoremediation(M), and plant-assisted myc...A 120-day experiment was conducted to compare the removal of polycyclic aromatic hydrocarbons(PAHs) from agricultural soil after natural attenuation(NA), phytoremediation(P), mycoremediation(M), and plant-assisted mycoremediation(PAM) approaches in relation to the extracellular enzyme activities in soil. The NA treatment removed the total soil PAH content negligibly. The P treatment using maize(Zea mays) enhanced only the removal of low and medium molecular PAHs. The Pleurotus ostreatus cultivated on 30–50 mm wood chip substrate used in M treatment was the most successful in the removal of majority PAHs. Therefore,significantly(p < 0.05) highest total PAH removal by 541.4 μg/kg dw(dry weight)(36%) from all tested M treatments was observed. When using the same fungal substrate together with maize in PAM treatment, the total PAH removal was not statistically different from the previous M treatment. However, the maize-assisted mycoremediation treatment significantly boosted fungal biomass, microbial and manganese peroxidase activity in soil which strongly correlated with the removal of total PAHs. The higher PAH removal in that PAM treatment could be reflected in the following post-harvest time. Our suggested M and PAM approaches could be promising in situ bioremediation strategies for PAH-contaminated soils.展开更多
文摘White rot fungus Pleurotus djamor H1 was stilly cultured at 28 ℃ under 4 different kinds of LNAS culture solution,the extracellular enzyme solutions were sampled at different interval,O.D.values of the solutions,representing manganese peroxidase(MnP),laccase and lignin peroxidase(LiP)activities,were measured spectrophotometrically by monitoring the oxidation of 2,6-DMP at 470 nm,ABTS at 420 nm and veratryl alcohol(VA)at 310 nm,the lignin-degrading enzymes of the fungus and the relationships between enzyme activities and medium composition and substrates were gained.Results indicated that Pleurotus djamor could produce MnP and laccase simultaneously,but no Lip.Substrates wood sawdust and 2,6-DMP could significantly enhanced MnP and laccase activities,the biggest Mnp and laccase activity were 5 U·L-1.and 38 U·L-1 when no substrates were added,and 69 U·L-1 and 205 U·L-1 when substrates added.The paper offered a basic ligninolytic enzymology of Pleurotus djamor for future utilization of the enzymes and further study on MnP and laccase mechanism of the fungus.
基金supported by the Ministry of Agriculture of the Czech Republic (QK1710379)the University-wide internal grant agency of CULS Prague – CIGA (20172018)
文摘A 120-day experiment was conducted to compare the removal of polycyclic aromatic hydrocarbons(PAHs) from agricultural soil after natural attenuation(NA), phytoremediation(P), mycoremediation(M), and plant-assisted mycoremediation(PAM) approaches in relation to the extracellular enzyme activities in soil. The NA treatment removed the total soil PAH content negligibly. The P treatment using maize(Zea mays) enhanced only the removal of low and medium molecular PAHs. The Pleurotus ostreatus cultivated on 30–50 mm wood chip substrate used in M treatment was the most successful in the removal of majority PAHs. Therefore,significantly(p < 0.05) highest total PAH removal by 541.4 μg/kg dw(dry weight)(36%) from all tested M treatments was observed. When using the same fungal substrate together with maize in PAM treatment, the total PAH removal was not statistically different from the previous M treatment. However, the maize-assisted mycoremediation treatment significantly boosted fungal biomass, microbial and manganese peroxidase activity in soil which strongly correlated with the removal of total PAHs. The higher PAH removal in that PAM treatment could be reflected in the following post-harvest time. Our suggested M and PAM approaches could be promising in situ bioremediation strategies for PAH-contaminated soils.
