BACKGROUND：Leptin regulates neuroendocrine function of the hypothalamus-pituitary-ovary axis in humans. OBJECTIVE： To verify effects of intracerebroventricular leptin injection on neuroendocrine function of the hypo...BACKGROUND：Leptin regulates neuroendocrine function of the hypothalamus-pituitary-ovary axis in humans. OBJECTIVE： To verify effects of intracerebroventricular leptin injection on neuroendocrine function of the hypothalamus-pituitary-ovary axis in ovariectomized rats. DESIGN, TIME AND SETTING： A randomized, controlled experiment was performed at the Basic Medical Institute, Chengde Medical College between June and October 2007. MATERIALS： Thirty healthy, female, Wistar rats were included in this study. The following compounds were used： leptin; gonadotropin-releasing hormone （GnRH） and luteinizing hormone （LH） enzyme-linked immunosorbent assay kits. METHODS： Rats were randomly divided into 3 groups at 1, 2, and 4 hours after injection. Each group was subdivided into control and experimental groups （n = 5 animals per group and time point）. All rats were subjected to bilateral ovariectomy and, beginning on day 7 after surgery, animals received daily subcutaneous injections of estradiol benzoate （2 μg） for 7 consecutive days. The experimental groups were injected with 5 μL leptin （1 g/L） into the lateral cerebral ventricle, and control groups received an equal volume of physiological saline. MAIN OUTCOME MEASURES： GnRH and LH secretion were examined 1, 2, and 4 hours after injection using GnRH and LH ELISA kits, respectively. RESULTS： In the experimental groups, GnRH secretion significantly increased （P 〈 0.01）, followed by LH secretion （P 〈 0.01）, compared with the control groups. GnRH secretion significantly increased 1 hour after leptin injection （P 〈 0.01）. The LH increase was less pronounced, but still significant （P 〈 0.01）; however, the most prominent LH increase occurred between 1 and 2 hours. Both GnRH and LH secretion reached peak levels at 2 hours after leptin injection. Thereafter, both GnRH and LH secretion decreased, but still maintained very high levels, compared with the control group （P 〈 0.01）. CONCLUSION： Intracerebroventricular展开更多
在大白鼠侧脑室(LV)注射碘化丙啶(PI)后,动物均表现明显异常,有躯体震颤、共济失调及眼球震颤等。脑组织内 PI 荧光的强度,是以注射部位及脑室为中心向脑表面的方向逐渐减弱,在前脑可达同侧尾、壳核的最外侧部。并选择性地标记隔区的隔...在大白鼠侧脑室(LV)注射碘化丙啶(PI)后,动物均表现明显异常,有躯体震颤、共济失调及眼球震颤等。脑组织内 PI 荧光的强度,是以注射部位及脑室为中心向脑表面的方向逐渐减弱,在前脑可达同侧尾、壳核的最外侧部。并选择性地标记隔区的隔海马核、中脑多巴胺(DA)细胞 A_(8-9-10)群、中缝背核、中缝正中核、中脑尾份内侧丘系内及其背侧的神经细胞,以及小脑的 Purkinje 细胞,均为双侧标记。其他部位则未见明显标记。结合 Faglu 荧光组织化学显示,几乎全部中脑 DA 细胞均显 PI 荧光。A_(8-9-10)内未见非 DA 细胞标记。在 LV 注射 PI 前切断一侧前脑内侧束,可见 PI 堆积于近切断处远端的儿茶酚胺纤维内,而近端无堆积。LV 注射伊凡思蓝(EB)、溴乙啶或 DAPI,动物均未出现明显异常。EB 和 DAPI 在脑内远隔部位虽也可见神经元标记,但其分布与 PI 的标记明显不同。上述结果除进一步证实了 LV 注射 PI,可引起动物明显的异常表现及选择性被 Purkinje 细胞摄取外,还表明,1.PI 能从脑脊液内进入脑实质,并在脑内广泛扩散;2.选择性地标记脑内一些特定部位的神经细胞,其中在 A_(8-9-10)内的标记细胞均系 DA神经元;3.这些 DA 细胞是通过轴突摄取 PI 及逆行轴浆运输而标记的。 ‘展开更多
目的:探讨川芎嗪对大鼠心脏血流动力学作用及机制。方法:以左心室收缩压(LVSP)、左心室终末舒张压(LVEDP)、左心室最大变化速度(±dp/dt max)为指标,在氨基甲酸乙酯麻醉的 SD 大鼠上观察川芎嗪对上述指标的影响。结果:侧脑室注射川...目的:探讨川芎嗪对大鼠心脏血流动力学作用及机制。方法:以左心室收缩压(LVSP)、左心室终末舒张压(LVEDP)、左心室最大变化速度(±dp/dt max)为指标,在氨基甲酸乙酯麻醉的 SD 大鼠上观察川芎嗪对上述指标的影响。结果:侧脑室注射川芎嗪后大鼠LVSP、LVDEP、±dp/dt max 明显增加,与生理盐水组和给药前比较差异均有显著性(P<0.01)。侧脑预先注射β受体阻断剂心得安可部分阻断川芎嗪增强 LVSP、LVEDP、±dp/dt max 的作用。结论:川芎嗪有明显增强 LVSP、LVEDP、±dp/dt max 的作用。川芎嗪增强心血管系统的作用可能部分由脑内β受体介导。展开更多
基金Science Foundation of Hebei Provincial Science & Technology Department, No.08726101D-20Science Foundation of Hebei Provincial Education Department, No. 2008301
文摘BACKGROUND：Leptin regulates neuroendocrine function of the hypothalamus-pituitary-ovary axis in humans. OBJECTIVE： To verify effects of intracerebroventricular leptin injection on neuroendocrine function of the hypothalamus-pituitary-ovary axis in ovariectomized rats. DESIGN, TIME AND SETTING： A randomized, controlled experiment was performed at the Basic Medical Institute, Chengde Medical College between June and October 2007. MATERIALS： Thirty healthy, female, Wistar rats were included in this study. The following compounds were used： leptin; gonadotropin-releasing hormone （GnRH） and luteinizing hormone （LH） enzyme-linked immunosorbent assay kits. METHODS： Rats were randomly divided into 3 groups at 1, 2, and 4 hours after injection. Each group was subdivided into control and experimental groups （n = 5 animals per group and time point）. All rats were subjected to bilateral ovariectomy and, beginning on day 7 after surgery, animals received daily subcutaneous injections of estradiol benzoate （2 μg） for 7 consecutive days. The experimental groups were injected with 5 μL leptin （1 g/L） into the lateral cerebral ventricle, and control groups received an equal volume of physiological saline. MAIN OUTCOME MEASURES： GnRH and LH secretion were examined 1, 2, and 4 hours after injection using GnRH and LH ELISA kits, respectively. RESULTS： In the experimental groups, GnRH secretion significantly increased （P 〈 0.01）, followed by LH secretion （P 〈 0.01）, compared with the control groups. GnRH secretion significantly increased 1 hour after leptin injection （P 〈 0.01）. The LH increase was less pronounced, but still significant （P 〈 0.01）; however, the most prominent LH increase occurred between 1 and 2 hours. Both GnRH and LH secretion reached peak levels at 2 hours after leptin injection. Thereafter, both GnRH and LH secretion decreased, but still maintained very high levels, compared with the control group （P 〈 0.01）. CONCLUSION： Intracerebroventricular
文摘在大白鼠侧脑室(LV)注射碘化丙啶(PI)后,动物均表现明显异常,有躯体震颤、共济失调及眼球震颤等。脑组织内 PI 荧光的强度,是以注射部位及脑室为中心向脑表面的方向逐渐减弱,在前脑可达同侧尾、壳核的最外侧部。并选择性地标记隔区的隔海马核、中脑多巴胺(DA)细胞 A_(8-9-10)群、中缝背核、中缝正中核、中脑尾份内侧丘系内及其背侧的神经细胞,以及小脑的 Purkinje 细胞,均为双侧标记。其他部位则未见明显标记。结合 Faglu 荧光组织化学显示,几乎全部中脑 DA 细胞均显 PI 荧光。A_(8-9-10)内未见非 DA 细胞标记。在 LV 注射 PI 前切断一侧前脑内侧束,可见 PI 堆积于近切断处远端的儿茶酚胺纤维内,而近端无堆积。LV 注射伊凡思蓝(EB)、溴乙啶或 DAPI,动物均未出现明显异常。EB 和 DAPI 在脑内远隔部位虽也可见神经元标记,但其分布与 PI 的标记明显不同。上述结果除进一步证实了 LV 注射 PI,可引起动物明显的异常表现及选择性被 Purkinje 细胞摄取外,还表明,1.PI 能从脑脊液内进入脑实质,并在脑内广泛扩散;2.选择性地标记脑内一些特定部位的神经细胞,其中在 A_(8-9-10)内的标记细胞均系 DA神经元;3.这些 DA 细胞是通过轴突摄取 PI 及逆行轴浆运输而标记的。 ‘
文摘目的:探讨川芎嗪对大鼠心脏血流动力学作用及机制。方法:以左心室收缩压(LVSP)、左心室终末舒张压(LVEDP)、左心室最大变化速度(±dp/dt max)为指标,在氨基甲酸乙酯麻醉的 SD 大鼠上观察川芎嗪对上述指标的影响。结果:侧脑室注射川芎嗪后大鼠LVSP、LVDEP、±dp/dt max 明显增加,与生理盐水组和给药前比较差异均有显著性(P<0.01)。侧脑预先注射β受体阻断剂心得安可部分阻断川芎嗪增强 LVSP、LVEDP、±dp/dt max 的作用。结论:川芎嗪有明显增强 LVSP、LVEDP、±dp/dt max 的作用。川芎嗪增强心血管系统的作用可能部分由脑内β受体介导。
