Monosomic lines of Nicotiana tabacum are helpful to confirm the location of genes on specific chromosomes. In the cross N. nudicaulis and N. tabacum, hybrid seedlings express lethal symptoms, which are controlled by t...Monosomic lines of Nicotiana tabacum are helpful to confirm the location of genes on specific chromosomes. In the cross N. nudicaulis and N. tabacum, hybrid seedlings express lethal symptoms, which are controlled by the S subgenome of N. tabacum. To identify the responsible chromosome, we needed to produce chromosome lacking lines (CLLs) of N. tabacum L. “Red Russian” and use them to cross with N. nudicaulis. From a cross of (N. tabacum × N. tomentosiformis) × N. tabacum, 380 BC1 individuals were obtained. Using a Haplo-Q line (a monosomic line lacking the single linkage group 11) and N. tabacum, we found that qPCR is a simple and reliable screening method for CLLs of N. tabacum. The marker PT30342 is located on linkage group 11, and the -Ct value (Ct Actin - Ct PT30342) was 2.0 for a disomic line and was 1.097 for a Haplo-Q line. By the use of flow cytometry, qPCR and chromosome counting together as a screening method, we identified 6 CLLs lacking 2 to 6 chromosomes. Compared with conventional methods, our method is a rapid technique for making and screening CLLs ofthe S or S/T subgenome of N. tabacum. Further, these CLLs will be useful to identify the location of two or more factors on chromosomes controlling a variety of genetic problems affecting breeding. Here, we only made CLLs of the S or S/T subgenome of N. tabacum. We will use the method established in this study to produce CLLs of the T subgenome of N. tabacum, and gather a full set of CLLs of N. tabacum. qPCR could also be applied to the identification of chromosome aberrations in other plants.展开更多
Schreinemakers bundle describes the relative arrangement of the univariant lines around an invariant point, but in practice choice cannot be often determined between a given sequence of the univariant lines and its mi...Schreinemakers bundle describes the relative arrangement of the univariant lines around an invariant point, but in practice choice cannot be often determined between a given sequence of the univariant lines and its mirror image due to lacking of entropy data. Some properties of PT bundle will be dealt with in this paper when entropy of one of the phases involved is unknown.展开更多
Mo-deficiency exercises a conspicuous effect on the NR activity of plant tissues, and little detectable NR activity develops in plant tissues lacking Mo. Many evidences indicate that an addition of Mo to Mo-deficient ...Mo-deficiency exercises a conspicuous effect on the NR activity of plant tissues, and little detectable NR activity develops in plant tissues lacking Mo. Many evidences indicate that an addition of Mo to Mo-deficient nutrient solution can induce the restoration of NR activity in such plant tissues.展开更多
[Objective] Long-term （over 18 years） fertilization experiments were con- ducted to study the responses of crop yields and soil fertility to long-term nutrient lacking at Zhengzhou in China. [Method] The present stu...[Objective] Long-term （over 18 years） fertilization experiments were con- ducted to study the responses of crop yields and soil fertility to long-term nutrient lacking at Zhengzhou in China. [Method] The present study consisted of five treat- ments： 1 CK （no fertilizer or manure）, （2） NP （nitrogen and phosphorus fertilizer applied）, 31 NK （nitrogen and potassium fertilizer applied）, 4 PK （phosphorus and potassium fertilizer applied） and ：5 NPK （nitrogen, phosphorus and potassium fertil- izer applied）. [Result] Lacking of nitrogen or phosphorus led to a low yield; however, there was no significant difference in grain yields between the NP and NPK treat- ments which maintained a higher yield. Receiving no phosphorus, soil available phosphorus content declined to about 2.5 mg/kg. The concentration of soil ex- changeable potassium remained constant at a level of 60 mg/kg under the treat- ments without potassium fertilizer addition. Soil potassium spontaneous supply ca- pacity fluctuated around 100%.[Conclusion] In fluvo-aquic soil, nitrogen and phos- phorus were two key limiting factors to grain yields, biomass and yield component factors of wheat and maize, while potassium was not. However, potassium defi- ciency may occur in the future if there was still no potassium fertilizer applied.展开更多
文摘Monosomic lines of Nicotiana tabacum are helpful to confirm the location of genes on specific chromosomes. In the cross N. nudicaulis and N. tabacum, hybrid seedlings express lethal symptoms, which are controlled by the S subgenome of N. tabacum. To identify the responsible chromosome, we needed to produce chromosome lacking lines (CLLs) of N. tabacum L. “Red Russian” and use them to cross with N. nudicaulis. From a cross of (N. tabacum × N. tomentosiformis) × N. tabacum, 380 BC1 individuals were obtained. Using a Haplo-Q line (a monosomic line lacking the single linkage group 11) and N. tabacum, we found that qPCR is a simple and reliable screening method for CLLs of N. tabacum. The marker PT30342 is located on linkage group 11, and the -Ct value (Ct Actin - Ct PT30342) was 2.0 for a disomic line and was 1.097 for a Haplo-Q line. By the use of flow cytometry, qPCR and chromosome counting together as a screening method, we identified 6 CLLs lacking 2 to 6 chromosomes. Compared with conventional methods, our method is a rapid technique for making and screening CLLs ofthe S or S/T subgenome of N. tabacum. Further, these CLLs will be useful to identify the location of two or more factors on chromosomes controlling a variety of genetic problems affecting breeding. Here, we only made CLLs of the S or S/T subgenome of N. tabacum. We will use the method established in this study to produce CLLs of the T subgenome of N. tabacum, and gather a full set of CLLs of N. tabacum. qPCR could also be applied to the identification of chromosome aberrations in other plants.
文摘Schreinemakers bundle describes the relative arrangement of the univariant lines around an invariant point, but in practice choice cannot be often determined between a given sequence of the univariant lines and its mirror image due to lacking of entropy data. Some properties of PT bundle will be dealt with in this paper when entropy of one of the phases involved is unknown.
文摘Mo-deficiency exercises a conspicuous effect on the NR activity of plant tissues, and little detectable NR activity develops in plant tissues lacking Mo. Many evidences indicate that an addition of Mo to Mo-deficient nutrient solution can induce the restoration of NR activity in such plant tissues.
基金Support by the Special Fund for Agro-scientific Research in the Public Interest of China(201203030-5)National Natural Science Foundation of China(41201288,41201255,31301284)+2 种基金Key Programs for Science and Technology Development of Henan Province(132102110068)Excellent Youth Science and Technology Foundation of Henan Academy of Agricultural Sciences(2013YQ15)JIRCAS-IARRP collaborative research:Estimation of the Present States of Fertilizer Use and Livestock Production and Their Environmental Load~~
文摘[Objective] Long-term （over 18 years） fertilization experiments were con- ducted to study the responses of crop yields and soil fertility to long-term nutrient lacking at Zhengzhou in China. [Method] The present study consisted of five treat- ments： 1 CK （no fertilizer or manure）, （2） NP （nitrogen and phosphorus fertilizer applied）, 31 NK （nitrogen and potassium fertilizer applied）, 4 PK （phosphorus and potassium fertilizer applied） and ：5 NPK （nitrogen, phosphorus and potassium fertil- izer applied）. [Result] Lacking of nitrogen or phosphorus led to a low yield; however, there was no significant difference in grain yields between the NP and NPK treat- ments which maintained a higher yield. Receiving no phosphorus, soil available phosphorus content declined to about 2.5 mg/kg. The concentration of soil ex- changeable potassium remained constant at a level of 60 mg/kg under the treat- ments without potassium fertilizer addition. Soil potassium spontaneous supply ca- pacity fluctuated around 100%.[Conclusion] In fluvo-aquic soil, nitrogen and phos- phorus were two key limiting factors to grain yields, biomass and yield component factors of wheat and maize, while potassium was not. However, potassium defi- ciency may occur in the future if there was still no potassium fertilizer applied.
