In the last fewyears,3D printing has emerged as a promising alternative for the fabrication ofmicrofluidic devices,overcoming some of the limitations associated with conventional soft-lithography.Stereolithography(SLA...In the last fewyears,3D printing has emerged as a promising alternative for the fabrication ofmicrofluidic devices,overcoming some of the limitations associated with conventional soft-lithography.Stereolithography(SLA),extrusion-based technology,and inkjet 3D printing are three of the widely used 3D printing technologies owing to their accessibility and affordability.Microfluidic devices can be 3D printed by employing a manufacturing approach from four fundamental manufacturing approaches classified as(1)direct printing approach,(2)mold-based approach,(3)modular approach,and(4)hybrid approach.To evaluate the feasibility of 3D printing technologies for fabricating microfluidic devices,a review focused on 3D printing fundamental manufacturing approaches has been presented.Using a broad spectrum of additive manufacturing materials,3D printed microfluidic devices have been implemented in various fields,including biological,chemical,and material synthesis.However,some crucial challenges are associated with the same,including low resolution,low optical transparency,cytotoxicity,high surface roughness,autofluorescence,non-compatibility with conventional sterilization methods,and low gas permeability.The recent research progress in materials related to additive manufacturing has aided in overcoming some of these challenges.Lastly,we outline possible implications of 3D printed microfluidics on the various fields of healthcare such as in vitro disease modeling and organ modeling,novel drug development,personalized treatment for cancer,and cancer drug screening by discussing the current state and future outlook of 3D printed‘organs-on-chips,’and 3D printed‘tumor-on-chips.’We conclude the review by highlighting future research directions in this field.展开更多
Lab-on-a-Chip(LoC)devices are extremely promising in that they enable diagnostic functions at the point-of-care.Within this scope,an important goal is to design imaging schemes that can be used out of the laboratory.I...Lab-on-a-Chip(LoC)devices are extremely promising in that they enable diagnostic functions at the point-of-care.Within this scope,an important goal is to design imaging schemes that can be used out of the laboratory.In this paper,we introduce and test a pocket holographic slide that allows digital holography microscopy to be performed without an interferometer setup.Instead,a commercial off-the-shelf plastic chip is engineered and functionalized with this aim.The microfluidic chip is endowed with micro-optics,that is,a diffraction grating and polymeric lenses,to build an interferometer directly on the chip,avoiding the need for a reference arm and external bulky optical components.Thanks to the single-beam scheme,the system is completely integrated and robust against vibrations,sharing the useful features of any common path interferometer.Hence,it becomes possible to bring holographic functionalities out of the lab,moving complexity from the external optical apparatus to the chip itself.Label-free imaging and quantitative phase contrast mapping of live samples are demonstrated,along with flexible refocusing capabilities.Thus,a liquid volume can be analyzed in one single shot with no need for mechanical scanning systems.展开更多
基金This work was supported by the Prime Minister’s Research Fellowship(PMRF)provided by the Ministry of Human Resource Development(MHRD,Govt.of India).
文摘In the last fewyears,3D printing has emerged as a promising alternative for the fabrication ofmicrofluidic devices,overcoming some of the limitations associated with conventional soft-lithography.Stereolithography(SLA),extrusion-based technology,and inkjet 3D printing are three of the widely used 3D printing technologies owing to their accessibility and affordability.Microfluidic devices can be 3D printed by employing a manufacturing approach from four fundamental manufacturing approaches classified as(1)direct printing approach,(2)mold-based approach,(3)modular approach,and(4)hybrid approach.To evaluate the feasibility of 3D printing technologies for fabricating microfluidic devices,a review focused on 3D printing fundamental manufacturing approaches has been presented.Using a broad spectrum of additive manufacturing materials,3D printed microfluidic devices have been implemented in various fields,including biological,chemical,and material synthesis.However,some crucial challenges are associated with the same,including low resolution,low optical transparency,cytotoxicity,high surface roughness,autofluorescence,non-compatibility with conventional sterilization methods,and low gas permeability.The recent research progress in materials related to additive manufacturing has aided in overcoming some of these challenges.Lastly,we outline possible implications of 3D printed microfluidics on the various fields of healthcare such as in vitro disease modeling and organ modeling,novel drug development,personalized treatment for cancer,and cancer drug screening by discussing the current state and future outlook of 3D printed‘organs-on-chips,’and 3D printed‘tumor-on-chips.’We conclude the review by highlighting future research directions in this field.
基金supported by IC+project:Imaging Citometry in PLastic Ultramobile Systems in the framework of Progetto Bandiera‘La Fabbrica del Futuro Piattaforma Manifatturiera Nazionale.’。
文摘Lab-on-a-Chip(LoC)devices are extremely promising in that they enable diagnostic functions at the point-of-care.Within this scope,an important goal is to design imaging schemes that can be used out of the laboratory.In this paper,we introduce and test a pocket holographic slide that allows digital holography microscopy to be performed without an interferometer setup.Instead,a commercial off-the-shelf plastic chip is engineered and functionalized with this aim.The microfluidic chip is endowed with micro-optics,that is,a diffraction grating and polymeric lenses,to build an interferometer directly on the chip,avoiding the need for a reference arm and external bulky optical components.Thanks to the single-beam scheme,the system is completely integrated and robust against vibrations,sharing the useful features of any common path interferometer.Hence,it becomes possible to bring holographic functionalities out of the lab,moving complexity from the external optical apparatus to the chip itself.Label-free imaging and quantitative phase contrast mapping of live samples are demonstrated,along with flexible refocusing capabilities.Thus,a liquid volume can be analyzed in one single shot with no need for mechanical scanning systems.
