In this study, we aimed to clarify the source of the reactive oxygen species （ROS） generation induced by vanadium compounds. We used vanadyl acetylacetonate （VO（acac）2）, a highly effective agent in controlling h...In this study, we aimed to clarify the source of the reactive oxygen species （ROS） generation induced by vanadium compounds. We used vanadyl acetylacetonate （VO（acac）2）, a highly effective agent in controlling hyperglycemia, to determine the source of ROS generation in two renal cell lines LLC-PK1 and MDCK. Four commonly fluorescent dyes were used to assess VO（acac）2-induced H202 and ＂02 production and their location. It demonstrated that VO（acac）2 can induce significant ROS generation in both LLC-PKI and MDCK cells, which were primarily derived from mitochondria. The results obtained in this study raised the possibility to reduce ROS level induced by vanadium compounds locally and thus avoid affecting its activity.展开更多
基金National Natural Science Foundation of China(Grant No. 20871008 and 21171011)
文摘In this study, we aimed to clarify the source of the reactive oxygen species （ROS） generation induced by vanadium compounds. We used vanadyl acetylacetonate （VO（acac）2）, a highly effective agent in controlling hyperglycemia, to determine the source of ROS generation in two renal cell lines LLC-PK1 and MDCK. Four commonly fluorescent dyes were used to assess VO（acac）2-induced H202 and ＂02 production and their location. It demonstrated that VO（acac）2 can induce significant ROS generation in both LLC-PKI and MDCK cells, which were primarily derived from mitochondria. The results obtained in this study raised the possibility to reduce ROS level induced by vanadium compounds locally and thus avoid affecting its activity.
