Fluorescence microscopy is a powerful tool for scientists to observe the microscopic world,and the fluorescence excitation light source is one of the most critical components.To compensate for the short operation life...Fluorescence microscopy is a powerful tool for scientists to observe the microscopic world,and the fluorescence excitation light source is one of the most critical components.To compensate for the short operation lifetime,integrated light sources,and low excitation efficiency of conventional light sources such as mercury,halogen,and xenon lamps,we designed an LED-integrated excitation cube(LEC)with a decentralized structure and high optical power density.Using a Fresnel lens,the light from the light-emitting diode(LED)was effectively focused within a 15 mm mounting distance to achieve high-efficiency illumination.LEC can be easily designed in the shape of fluorescence filter cubes for installation in commercial fluorescence microscopes.LECs’optical efficiency is 1–2 orders of magnitude higher than that of mercury lamps;therefore,high-quality fluorescence imaging with spectral coverage from UV to red can be achieved.By replacing conventional fluorescence filter cubes,LEC can be easily installed on any commercial fluorescence microscope.A built-in LEC driver can identify the types of LEDs in different spectral bands to adopt the optimal operating current and frequency of pulses.Moreover,high-contrast images can be achieved in pulse mode by time-gated imaging of long-lifetime luminescence.展开更多
A series of phosphor samples of 2(Sr_(1- x )M_ x O)·(1- y )P_2O_5· y B_2O_3∶Eu (2+) (M=Mg,0≤ x ≤1,0< y <0.5) were prepared and the emission spectra,excitation spectra and X-ray diffraction spectra w...A series of phosphor samples of 2(Sr_(1- x )M_ x O)·(1- y )P_2O_5· y B_2O_3∶Eu (2+) (M=Mg,0≤ x ≤1,0< y <0.5) were prepared and the emission spectra,excitation spectra and X-ray diffraction spectra were studied. It is found that the position of the emission peak located near 478 nm is not changeable as y is varied. Otherwise the relative intensity of the peak is different distinctly and the relative intensity reaches maximum when y is equal to 0.16. The introduction of Mg into 2SrO·0.84P_2O_5·0.16B_2O_3∶Eu (2+) gives rise to a shifting of the position of the emission peaks under UV excitation. The emission peak wavelength decreases from 478 to 420 nm with the increase of x ,whereas the brightness decreases simultaneously. Furthermore the intensity of the excitation spectra decreases observably and the excitation band shifts to shorter wavelength identically.展开更多
基金the Shenzhen Science and Technology Program(KQTD20170810110913065 and 20200925174735005).
文摘Fluorescence microscopy is a powerful tool for scientists to observe the microscopic world,and the fluorescence excitation light source is one of the most critical components.To compensate for the short operation lifetime,integrated light sources,and low excitation efficiency of conventional light sources such as mercury,halogen,and xenon lamps,we designed an LED-integrated excitation cube(LEC)with a decentralized structure and high optical power density.Using a Fresnel lens,the light from the light-emitting diode(LED)was effectively focused within a 15 mm mounting distance to achieve high-efficiency illumination.LEC can be easily designed in the shape of fluorescence filter cubes for installation in commercial fluorescence microscopes.LECs’optical efficiency is 1–2 orders of magnitude higher than that of mercury lamps;therefore,high-quality fluorescence imaging with spectral coverage from UV to red can be achieved.By replacing conventional fluorescence filter cubes,LEC can be easily installed on any commercial fluorescence microscope.A built-in LEC driver can identify the types of LEDs in different spectral bands to adopt the optimal operating current and frequency of pulses.Moreover,high-contrast images can be achieved in pulse mode by time-gated imaging of long-lifetime luminescence.
基金The Joint Fund of Chinese Academy of Sciences(6141A01080302)National Key Research and Development Program of China(2016YFB0402101)+2 种基金University Natural Science Research Project of Anhui Province of China(KJ2019ZD06)National Natural Science Foundation of China(51702322,51802307)Fund of Key Laboratory of Optoelectronic Materials Chemistry and Physics,Chinese Academy of Sciences(2008DP173016)。
文摘A series of phosphor samples of 2(Sr_(1- x )M_ x O)·(1- y )P_2O_5· y B_2O_3∶Eu (2+) (M=Mg,0≤ x ≤1,0< y <0.5) were prepared and the emission spectra,excitation spectra and X-ray diffraction spectra were studied. It is found that the position of the emission peak located near 478 nm is not changeable as y is varied. Otherwise the relative intensity of the peak is different distinctly and the relative intensity reaches maximum when y is equal to 0.16. The introduction of Mg into 2SrO·0.84P_2O_5·0.16B_2O_3∶Eu (2+) gives rise to a shifting of the position of the emission peaks under UV excitation. The emission peak wavelength decreases from 478 to 420 nm with the increase of x ,whereas the brightness decreases simultaneously. Furthermore the intensity of the excitation spectra decreases observably and the excitation band shifts to shorter wavelength identically.
