The reactions of carboplatin ([Pt(NH3)2(CBDCA)], in which CBDCA is cyclobutane 1,1, dicarboxylate) with L selenomethionine (L Se MetH) and L methionine (L MetH) were investigated using electrospray mass spectroscopy (...The reactions of carboplatin ([Pt(NH3)2(CBDCA)], in which CBDCA is cyclobutane 1,1, dicarboxylate) with L selenomethionine (L Se MetH) and L methionine (L MetH) were investigated using electrospray mass spectroscopy (ESMS) and 2D HSQC NMR spectroscopy. The dominant products for both reactions are bis chelated complexes [Pt(L Se Met)2] and [Pt(L Met)2]. These results are different from those observed for the reactions of cisplatin ([PtCl2(NH3)]) with L Se MetH or L MetH, in which the main products are mono chelated complexes [PtCl(NH3)(L Se Met)] or [PtCl(NH3)(L Met)]. This work provides insights into the differences between cisplatin and carboplatin in their reactivity and mechanism of reaction.展开更多
以L-硒代蛋氨酸、月桂酰氯和油酰氯为主要原料通过肖顿-鲍曼缩合和酯化反应得到了N-酰基-L-硒代蛋氨酸衍生物,产物运用核磁共振(NMR)技术(1H NMR,13 C NMR)表征确认。采用噻唑蓝(MTT)比色法研究了目标化合物对人体正常肝细胞(HL-7702)...以L-硒代蛋氨酸、月桂酰氯和油酰氯为主要原料通过肖顿-鲍曼缩合和酯化反应得到了N-酰基-L-硒代蛋氨酸衍生物,产物运用核磁共振(NMR)技术(1H NMR,13 C NMR)表征确认。采用噻唑蓝(MTT)比色法研究了目标化合物对人体正常肝细胞(HL-7702)和大鼠肾小管上皮细胞(NRK-52E)的细胞毒性。结果表明,合成的目标化合物对人体正常肝细胞(HL-7702)和大鼠肾小管上皮细胞(NRK-52E)细胞毒性较低,当2种目标化合物的浓度达1920μmol/L时,细胞存活率均大于75%,证明合成的硒代蛋氨酸衍生物是一种低毒的硒代化合物,对于扩大硒代蛋氨酸在油脂类食品和农产品加工领域的应用具有一定潜力。展开更多
文摘The reactions of carboplatin ([Pt(NH3)2(CBDCA)], in which CBDCA is cyclobutane 1,1, dicarboxylate) with L selenomethionine (L Se MetH) and L methionine (L MetH) were investigated using electrospray mass spectroscopy (ESMS) and 2D HSQC NMR spectroscopy. The dominant products for both reactions are bis chelated complexes [Pt(L Se Met)2] and [Pt(L Met)2]. These results are different from those observed for the reactions of cisplatin ([PtCl2(NH3)]) with L Se MetH or L MetH, in which the main products are mono chelated complexes [PtCl(NH3)(L Se Met)] or [PtCl(NH3)(L Met)]. This work provides insights into the differences between cisplatin and carboplatin in their reactivity and mechanism of reaction.
文摘以L-硒代蛋氨酸、月桂酰氯和油酰氯为主要原料通过肖顿-鲍曼缩合和酯化反应得到了N-酰基-L-硒代蛋氨酸衍生物,产物运用核磁共振(NMR)技术(1H NMR,13 C NMR)表征确认。采用噻唑蓝(MTT)比色法研究了目标化合物对人体正常肝细胞(HL-7702)和大鼠肾小管上皮细胞(NRK-52E)的细胞毒性。结果表明,合成的目标化合物对人体正常肝细胞(HL-7702)和大鼠肾小管上皮细胞(NRK-52E)细胞毒性较低,当2种目标化合物的浓度达1920μmol/L时,细胞存活率均大于75%,证明合成的硒代蛋氨酸衍生物是一种低毒的硒代化合物,对于扩大硒代蛋氨酸在油脂类食品和农产品加工领域的应用具有一定潜力。
