This study investigated the influence of red,green,blue,and white paper bags on the free volatile compound development of Kyoho grape berries from green to harvest.Seven functional groups of volatiles were identified ...This study investigated the influence of red,green,blue,and white paper bags on the free volatile compound development of Kyoho grape berries from green to harvest.Seven functional groups of volatiles were identified during the development of Kyoho grape berries including esters,aldehydes,alcohols,terpenes,ketones,acids,and hydrocarbons.Esters and aldehydes were abundant in Kyoho grape berries,mainly represented by ethyl acetate,ethyl butyrate,and(E)-2-hexenal.They accumulated quickly after veraison and slightly decreased toward maturation.Red,green,blue,and white paper bags promoted the accumulation of esters and inhibited the accumulation of aldehydes,also inhibited the accumulation of alcohols,tepenes,ketones,and acids.Their effect from strong to weak was green,blue,red,and white paper bags.The expression profiles of genes in the lipoxygenase-hydroperoxide lyase(LOX-HPL) pathway were also analyzed and the results indicated that the regulation of red,green,blue,and white paper bags on aldehydes,alcohols,and esters volatile aromas was at transcriptional level.The results expanded our comprehension in grape aroma biosynthesis and berry bagging technique in table grape cultivation.展开更多
Aroma is an important quality trait of grapes and often the focus of consumers,viticulturists and grapevine breeders.Kyoho is a hybrid between Vitis vinifera and Vitis labrusca with a strawberry-like scent,while 87-1 ...Aroma is an important quality trait of grapes and often the focus of consumers,viticulturists and grapevine breeders.Kyoho is a hybrid between Vitis vinifera and Vitis labrusca with a strawberry-like scent,while 87-1 is an early-ripening mutant of Muscat hamburg,belonging to Vitis vinifera,with a rose scent.In this study,we compared their aroma compositions and concentrations during berry development by headspace-SPME combined with gas chromatography-mass spectrometry(GC-MS),and analyzed the expression differences of enzyme-encoding genes in the LOX-HPL,MEP and MVA metabolic pathways by qRT-PCR.Twelve esters were detected in Kyoho during the whole berry development and they were abundant after veraison,but no esters were detected in 87-1 berries.Linalool was the dominant terpene among the 14 terpenes detected in 87-1 berries,while limited amounts of terpenes were detected in Kyoho berries.qRT-PCR analysis indicated that the low expression of VvAAT might explain the low content of ester volatiles in 87-1 berries,and the low expression of coding genes in the MEP pathway,especially VvPNLin Ner1,might be the reason for the low content of volatile terpenes in Kyoho berries.The results from this work will promote our understanding of aroma metabolic mechanisms of grapes,and offer some suggestions for grape aromatic quality improvement.展开更多
本试验以山葡萄、225RU、甜冬葡萄、5C、Vitis ripria Grand glober、5BB、桑叶葡萄、燕山葡萄、沙地葡萄、S04、Saltcreek、美洲葡萄、Dogridge、华东葡萄等14种野生葡萄和巨峰栽培葡萄为试材,进行硬枝扦插,观察其成活率、根系与枝蔓...本试验以山葡萄、225RU、甜冬葡萄、5C、Vitis ripria Grand glober、5BB、桑叶葡萄、燕山葡萄、沙地葡萄、S04、Saltcreek、美洲葡萄、Dogridge、华东葡萄等14种野生葡萄和巨峰栽培葡萄为试材,进行硬枝扦插,观察其成活率、根系与枝蔓生长情况。结果表明,SO4、5C两个类型的成活率、抽生的侧根和须根数以及华东葡萄的枝蔓生长明显优于其他野生葡萄。该项研究为砧木的选择及其快速繁殖提供了依据。展开更多
基金supported by the earmarked fund for China Agriculture Research System (CARS-30)the Introduction of International Advanced Agricultural Science and Technology Program Project,Ministry of Agriculture,China (948 Program,2011-G28)+1 种基金the National Key Technologies R&D Program of China during the 12th Five-Year Plan period (2014BAD16B05)the Agricultural Science and Technology Innovation Program,Chinese Academy of Agricultural Sciences (CAAS-ASTIP-2015-RIP-04)
文摘This study investigated the influence of red,green,blue,and white paper bags on the free volatile compound development of Kyoho grape berries from green to harvest.Seven functional groups of volatiles were identified during the development of Kyoho grape berries including esters,aldehydes,alcohols,terpenes,ketones,acids,and hydrocarbons.Esters and aldehydes were abundant in Kyoho grape berries,mainly represented by ethyl acetate,ethyl butyrate,and(E)-2-hexenal.They accumulated quickly after veraison and slightly decreased toward maturation.Red,green,blue,and white paper bags promoted the accumulation of esters and inhibited the accumulation of aldehydes,also inhibited the accumulation of alcohols,tepenes,ketones,and acids.Their effect from strong to weak was green,blue,red,and white paper bags.The expression profiles of genes in the lipoxygenase-hydroperoxide lyase(LOX-HPL) pathway were also analyzed and the results indicated that the regulation of red,green,blue,and white paper bags on aldehydes,alcohols,and esters volatile aromas was at transcriptional level.The results expanded our comprehension in grape aroma biosynthesis and berry bagging technique in table grape cultivation.
基金by the National Key R&D Program of China(2018YFD1000200)the Fundamental Research Funds for Central Non-profit Scientific Institution+1 种基金the Agricultural Science and Technology Innovation Program,Chinese Academy of Agricultural Sciences(CAAS-ASTIP2015-RIP-04)the earmarked fund for China Agriculture Research System(CARS-29-zp)。
文摘Aroma is an important quality trait of grapes and often the focus of consumers,viticulturists and grapevine breeders.Kyoho is a hybrid between Vitis vinifera and Vitis labrusca with a strawberry-like scent,while 87-1 is an early-ripening mutant of Muscat hamburg,belonging to Vitis vinifera,with a rose scent.In this study,we compared their aroma compositions and concentrations during berry development by headspace-SPME combined with gas chromatography-mass spectrometry(GC-MS),and analyzed the expression differences of enzyme-encoding genes in the LOX-HPL,MEP and MVA metabolic pathways by qRT-PCR.Twelve esters were detected in Kyoho during the whole berry development and they were abundant after veraison,but no esters were detected in 87-1 berries.Linalool was the dominant terpene among the 14 terpenes detected in 87-1 berries,while limited amounts of terpenes were detected in Kyoho berries.qRT-PCR analysis indicated that the low expression of VvAAT might explain the low content of ester volatiles in 87-1 berries,and the low expression of coding genes in the MEP pathway,especially VvPNLin Ner1,might be the reason for the low content of volatile terpenes in Kyoho berries.The results from this work will promote our understanding of aroma metabolic mechanisms of grapes,and offer some suggestions for grape aromatic quality improvement.
文摘本试验以山葡萄、225RU、甜冬葡萄、5C、Vitis ripria Grand glober、5BB、桑叶葡萄、燕山葡萄、沙地葡萄、S04、Saltcreek、美洲葡萄、Dogridge、华东葡萄等14种野生葡萄和巨峰栽培葡萄为试材,进行硬枝扦插,观察其成活率、根系与枝蔓生长情况。结果表明,SO4、5C两个类型的成活率、抽生的侧根和须根数以及华东葡萄的枝蔓生长明显优于其他野生葡萄。该项研究为砧木的选择及其快速繁殖提供了依据。
