Eight compounds were isolated from the fermentation cultures of rice sheath blight pathogen Rhizoctonia solani Kuhn. They were identified as ergosterol （1）, 6β-hydroxysitostenone （2）, sitostenone （3）, m-hydroxy...Eight compounds were isolated from the fermentation cultures of rice sheath blight pathogen Rhizoctonia solani Kuhn. They were identified as ergosterol （1）, 6β-hydroxysitostenone （2）, sitostenone （3）, m-hydroxyphenylacetic acid （4）, methyl m-hydroxyphenylacetate （5）, m-hydroxymethylphenyl pentanoate （6）, （Z）-3-methylpent-2-en-1,5-dioic acid （7） and 3-methoxyfuran-2-carboxylic acid （8） by means of physicochemical and spectroscopic analysis. Among them, 2, 3, 5-8 were isolated from R. solani for the first time. All the compounds were evaluated for their biological activities. 4-6 and 8 showed their inhibitory activities on the radical and germ elongation of rice seeds. 1,4 and 7 showed moderate antibacterial activity to some bacteria. 4, 7 and 8 exhibited weak inhibitory activities on spore germination of Magnaporthe oryzae. 8 showed moderate antioxidant activity with the 1,1-diphenyl-2-picryhydrazyl （DPPH） andβ-carotene-linoleic acid assays. This is the first time to reveal compounds 5, 6 and 8 from rice sheath blight pathogen R. solani to have in vitro phytotoxic activity.展开更多
To establish a standard system for geneticstudies on sheath blight resistance, a field testwas conducted at the experimental farm ofYangzhou University to compare several pro-cedures for inoculating rice plants with ... To establish a standard system for geneticstudies on sheath blight resistance, a field testwas conducted at the experimental farm ofYangzhou University to compare several pro-cedures for inoculating rice plants with R.solani Kuhn (RH9). The varieties used wereJasmine 85, Teqing (resistant or moderatelyresistant), and Lemont (susceptible). They展开更多
A strain of Streptomyces corchorusii （NY0919） antagonistic to Rhizoctonia solani Ktthn was isolated, which produced alkyl glycoside with high antifungal activity. To investigate the antifungal activity of Streptomyc...A strain of Streptomyces corchorusii （NY0919） antagonistic to Rhizoctonia solani Ktthn was isolated, which produced alkyl glycoside with high antifungal activity. To investigate the antifungal activity of Streptomyces corchorusii strain NF0919, the treatments amended with the non-sterilized supernatant resulted in the highest growth inhibition rate of about 100% at a concentration of 20%. The antifungal compound, which inhibited the growth of Rhizoctonia solani Kuhn, was extracted by EtOAC and purified by silica gel, Sephadex LH-20 column, and HPLC, where an active fraction was confirmed by LC-MS and NMR techniques. These results suggested that NF0919 had a high potential in the biocontrol of Rhizoctonia solani Kuhn, which was mainly due to surface active agent APG.展开更多
基金co-financed by the grants from the National Basic Resea rch Program of China (2013GB127800)the National Natural Science Foundation of China (31271996)
文摘Eight compounds were isolated from the fermentation cultures of rice sheath blight pathogen Rhizoctonia solani Kuhn. They were identified as ergosterol （1）, 6β-hydroxysitostenone （2）, sitostenone （3）, m-hydroxyphenylacetic acid （4）, methyl m-hydroxyphenylacetate （5）, m-hydroxymethylphenyl pentanoate （6）, （Z）-3-methylpent-2-en-1,5-dioic acid （7） and 3-methoxyfuran-2-carboxylic acid （8） by means of physicochemical and spectroscopic analysis. Among them, 2, 3, 5-8 were isolated from R. solani for the first time. All the compounds were evaluated for their biological activities. 4-6 and 8 showed their inhibitory activities on the radical and germ elongation of rice seeds. 1,4 and 7 showed moderate antibacterial activity to some bacteria. 4, 7 and 8 exhibited weak inhibitory activities on spore germination of Magnaporthe oryzae. 8 showed moderate antioxidant activity with the 1,1-diphenyl-2-picryhydrazyl （DPPH） andβ-carotene-linoleic acid assays. This is the first time to reveal compounds 5, 6 and 8 from rice sheath blight pathogen R. solani to have in vitro phytotoxic activity.
文摘 To establish a standard system for geneticstudies on sheath blight resistance, a field testwas conducted at the experimental farm ofYangzhou University to compare several pro-cedures for inoculating rice plants with R.solani Kuhn (RH9). The varieties used wereJasmine 85, Teqing (resistant or moderatelyresistant), and Lemont (susceptible). They
基金Supported by Agricultural Science and Technology Independent Innovation Fund of Jiangsu Province[CX(12)5067]
文摘A strain of Streptomyces corchorusii （NY0919） antagonistic to Rhizoctonia solani Ktthn was isolated, which produced alkyl glycoside with high antifungal activity. To investigate the antifungal activity of Streptomyces corchorusii strain NF0919, the treatments amended with the non-sterilized supernatant resulted in the highest growth inhibition rate of about 100% at a concentration of 20%. The antifungal compound, which inhibited the growth of Rhizoctonia solani Kuhn, was extracted by EtOAC and purified by silica gel, Sephadex LH-20 column, and HPLC, where an active fraction was confirmed by LC-MS and NMR techniques. These results suggested that NF0919 had a high potential in the biocontrol of Rhizoctonia solani Kuhn, which was mainly due to surface active agent APG.
