为了解青藏高原大骨节病流行区环境、食物及人群硒水平的分布现状,论文通过CNKI、Web of Science和Google Scholar等数据库检索收集了2000—2018年间发表的关于青藏高原大骨节病病区土壤、粮食、饮水及人发硒含量的文献,按拟定标准共筛...为了解青藏高原大骨节病流行区环境、食物及人群硒水平的分布现状,论文通过CNKI、Web of Science和Google Scholar等数据库检索收集了2000—2018年间发表的关于青藏高原大骨节病病区土壤、粮食、饮水及人发硒含量的文献,按拟定标准共筛选出33篇文献并提取有效数据进行统计分析。结果显示,青藏高原大骨节病病区自然环境总体仍处于低硒循环状态,其中耕作土壤、饮水平均硒含量分别为0.147 mg·kg-1和0.54μg·L-1,高原自产青稞、小麦、糌粑平均含硒量分别为9.27、19.08、11.07μg·kg-1;而病区儿童整体硒营养水平较1990年以前有明显升高趋势,发硒平均含量为0.234 mg·kg-1,已基本脱离硒缺乏状态(<0.20 mg·kg-1);外源性大米的硒含量为43.29μg·kg-1;病区儿童发硒水平与土壤总硒含量无明显相关关系(r=0.125,P>0.05,N=23)。青藏高原大骨节病流行区儿童发硒水平与自然环境硒水平不一致的变化趋势提示,随着社会人文因素的干预,外源性硒的输入有所增加,并在一定程度上降低了病区人群对当地低硒环境的依赖性,这可能是青藏高原大骨节病病情稳定下降并得到有效控制的主要原因。展开更多
Objective：To identify the in vitro effects of sodium hyaluronate（HA） on the proliferation and the apoptosis of chondrocytes from patients with Kashin-Beck disease（KBD） and osteoarthritis（OA）. Methods：Samples o...Objective：To identify the in vitro effects of sodium hyaluronate（HA） on the proliferation and the apoptosis of chondrocytes from patients with Kashin-Beck disease（KBD） and osteoarthritis（OA）. Methods：Samples of articular cartilages from KBD and OA patients, as well as healthy volunteers（6 subjects in each of the 3 groups） were dissected, digested with collagenase and the cells cultured in monolayers. Chondrocytes from each sample were assigned to an untreated group and two HA-treated groups： H0（no HA）, H100（HA, 0.1 g/L） and H500（HA, 0.5 g/L）. The first passage chondrocytes were used to observe proliferation using the MTT assay, and apoptosis by flow cytometry through Annexin V/PI staining. Results：HA promoted proliferation of chondrocytes in all the three groups, and.in KBD and OA groups, for cells cultured for 4 and 6 days, H500 significantly promoted the cell proliferation. The apoptotic rates of both KBD and OA group chondrocytes were in the order H500 〈 HA100 〈 H0. Conclusion：Sodium hyaluronate administration has a dosedependent in vitro effect to promote proliferation and inhibit apoptosis of chondrocytes from patients with KBD and OA.展开更多
目的描述陕西地区大骨节病(Kashin-Beck disease,KBD)患者膝、踝关节活动度(range of motion,ROM)和肌肉萎缩相关指标的分布特征,探究膝、踝ROM和肌肉萎缩指标的相关性,为KBD患者临床特征分析提供可参考依据。方法对来自陕西省KBD病区在...目的描述陕西地区大骨节病(Kashin-Beck disease,KBD)患者膝、踝关节活动度(range of motion,ROM)和肌肉萎缩相关指标的分布特征,探究膝、踝ROM和肌肉萎缩指标的相关性,为KBD患者临床特征分析提供可参考依据。方法对来自陕西省KBD病区在册KBD患者进行抽样调查。采集KBD患者一般人口学资料,测量患者膝关节(屈曲、伸展),踝关节(背屈、跖屈)活动度以及患者上臂围、大腿围、小腿围和握力等肌肉萎缩相关指标。按照人群特征[性别、年龄、体质量指数(BMI)]和KBD分度分组,报告KBD患者ROM和肌肉萎缩指标的中位数和四分位数,分析不同组间各指标的差异。控制性别、年龄和BMI等变量后,采用偏相关分析探索各指标间的相关性。结果本研究共调查KBD患者480例,其中男性249(51.9%)例,女性231(48.1%)例,平均年龄(63.10±7.32)岁,平均BMI(23.49±8.90)kg/m^(2)。结果显示,KBD患者膝关节屈曲活动度[105.0(95.0,120.0)]°,膝关节伸展活动度[0.0(-15.0,0.0)]°,踝关节背屈活动度[5.0(0.0,15.0)]°,踝关节跖屈活动度[20.0(15.0,30.0)]°,左侧大腿围[43.0(40.0,47.0)]cm,右侧大腿围[43.0(39.0,47.0)]cm,左侧小腿围[29.0(27.0,32.0)]cm,右侧小腿围[29.5(27.0,32.0)]cm,左侧和右侧上臂围均为[27.0(25.0,30.0)]cm,左手握力[13.4(9.5,18.4)]kg,右手握力[13.9(9.8,18.2)]kg。随着年龄的增加,KBD患者两侧膝关节伸展活动度呈现降低趋势(H=31.499、31.847;P均<0.001)。BMI正常组两侧膝关节屈曲活动度高于超重或肥胖组,差异均有统计学意义(H=7.753、12.333;P=0.021、0.002)。不同KBD分度下,两侧膝关节屈曲、大腿围和小腿围均呈现降低趋势(H=14.345、17.256、8.000、8.462、8.558、9.633;P均<0.05)。相关性分析显示,KBD患者膝关节屈曲活动度与大腿围、小腿围和握力呈正相关关系(P均<0.05)。KBD患者膝关节伸展活动度与大腿围呈正相关关系(P均<0.01)。结论陕西省KBD患者ROM受损和肌肉萎缩较为严�展开更多
基金supported by the Ministry of Science and Technology(2006 DFA33610)the Natural Scientific Fund of China(30630058)the International Co-operative Fund in Shaanxi(2005KW-13)
文摘Objective：To identify the in vitro effects of sodium hyaluronate（HA） on the proliferation and the apoptosis of chondrocytes from patients with Kashin-Beck disease（KBD） and osteoarthritis（OA）. Methods：Samples of articular cartilages from KBD and OA patients, as well as healthy volunteers（6 subjects in each of the 3 groups） were dissected, digested with collagenase and the cells cultured in monolayers. Chondrocytes from each sample were assigned to an untreated group and two HA-treated groups： H0（no HA）, H100（HA, 0.1 g/L） and H500（HA, 0.5 g/L）. The first passage chondrocytes were used to observe proliferation using the MTT assay, and apoptosis by flow cytometry through Annexin V/PI staining. Results：HA promoted proliferation of chondrocytes in all the three groups, and.in KBD and OA groups, for cells cultured for 4 and 6 days, H500 significantly promoted the cell proliferation. The apoptotic rates of both KBD and OA group chondrocytes were in the order H500 〈 HA100 〈 H0. Conclusion：Sodium hyaluronate administration has a dosedependent in vitro effect to promote proliferation and inhibit apoptosis of chondrocytes from patients with KBD and OA.
文摘目的描述陕西地区大骨节病(Kashin-Beck disease,KBD)患者膝、踝关节活动度(range of motion,ROM)和肌肉萎缩相关指标的分布特征,探究膝、踝ROM和肌肉萎缩指标的相关性,为KBD患者临床特征分析提供可参考依据。方法对来自陕西省KBD病区在册KBD患者进行抽样调查。采集KBD患者一般人口学资料,测量患者膝关节(屈曲、伸展),踝关节(背屈、跖屈)活动度以及患者上臂围、大腿围、小腿围和握力等肌肉萎缩相关指标。按照人群特征[性别、年龄、体质量指数(BMI)]和KBD分度分组,报告KBD患者ROM和肌肉萎缩指标的中位数和四分位数,分析不同组间各指标的差异。控制性别、年龄和BMI等变量后,采用偏相关分析探索各指标间的相关性。结果本研究共调查KBD患者480例,其中男性249(51.9%)例,女性231(48.1%)例,平均年龄(63.10±7.32)岁,平均BMI(23.49±8.90)kg/m^(2)。结果显示,KBD患者膝关节屈曲活动度[105.0(95.0,120.0)]°,膝关节伸展活动度[0.0(-15.0,0.0)]°,踝关节背屈活动度[5.0(0.0,15.0)]°,踝关节跖屈活动度[20.0(15.0,30.0)]°,左侧大腿围[43.0(40.0,47.0)]cm,右侧大腿围[43.0(39.0,47.0)]cm,左侧小腿围[29.0(27.0,32.0)]cm,右侧小腿围[29.5(27.0,32.0)]cm,左侧和右侧上臂围均为[27.0(25.0,30.0)]cm,左手握力[13.4(9.5,18.4)]kg,右手握力[13.9(9.8,18.2)]kg。随着年龄的增加,KBD患者两侧膝关节伸展活动度呈现降低趋势(H=31.499、31.847;P均<0.001)。BMI正常组两侧膝关节屈曲活动度高于超重或肥胖组,差异均有统计学意义(H=7.753、12.333;P=0.021、0.002)。不同KBD分度下,两侧膝关节屈曲、大腿围和小腿围均呈现降低趋势(H=14.345、17.256、8.000、8.462、8.558、9.633;P均<0.05)。相关性分析显示,KBD患者膝关节屈曲活动度与大腿围、小腿围和握力呈正相关关系(P均<0.05)。KBD患者膝关节伸展活动度与大腿围呈正相关关系(P均<0.01)。结论陕西省KBD患者ROM受损和肌肉萎缩较为严�
